Light sound acidity-basicity imaging method and apparatus based on reagent colour development difference

A technology of pH and reagents, applied in the field of detection, can solve problems such as low accuracy, inability to directly measure solid objects, and difficulty in color resolution, and achieve the effect of expanding the scope of application

Inactive Publication Date: 2009-05-27
SOUTH CHINA NORMAL UNIVERSITY
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  • Abstract
  • Description
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Problems solved by technology

[0002] At present, most of the measurement methods for the pH of the solution are manual methods, using pH test paper or indicators for measurement, but the color change range of test paper and indicators is judged by human eyes, resulting in low accuracy of this measurement method , especially for the change of the pH value below 0.5, it is quite difficult to judge the color by human eyes
And some pH value measuring instruments with electrodes as sensitive parts, although the measurement of the pH value of the solution is more accurate, and the volume is small, easy to carry, but these measuring instruments cannot directly measure solid objects, such as isolated meat, fruits, soil, etc.

Method used

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  • Light sound acidity-basicity imaging method and apparatus based on reagent colour development difference
  • Light sound acidity-basicity imaging method and apparatus based on reagent colour development difference
  • Light sound acidity-basicity imaging method and apparatus based on reagent colour development difference

Examples

Experimental program
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Effect test

Embodiment 1

[0034] A photoacoustic pH imaging device based on reagent color difference:

[0035] exist figure 1 In the above, the laser 1-1 emits pulsed laser light, which is reflected by the coating mirror 1-2 to the focusing lens 1-3 and then irradiates the sample to be tested to generate a photoacoustic signal. The laser 1-1 is a TII laser produced by the Belarusian LOTIS company that can emit continuously adjustable wavelengths of 350-500nm, 690nm-1000nm, and pulsed lasers of 532nm and 1064nm. The wavelength resolutions used by the corresponding photoacoustic pH detection reagent are 460nm and 532nm.

[0036]Acoustic signal collection is that the ultrasonic detector 3-2 amplifies the photoacoustic signal received by the detection signal amplifier 4-1 and then sends it to the digital oscilloscope 4-2 and is transmitted and collected by the digital acquisition card 4-3, and then stored in the computer 5 middle. The digital oscilloscope 4-2 is connected to the laser 1-1 at the same t...

Embodiment 2

[0039] Mix and dissolve 50 mg each of neutral red and methylene blue in 100 mL of ethanol solution, incorporate into multiple sets of pH standard solutions (PH: 4.0-10.0), and prepare 14 groups of photoacoustic acids with the same molar concentration and different pH values Alkali detection solution, the laser emits two wavelengths of laser light successively, and irradiates different groups of photoacoustic acid-base detection solutions; the solution absorbs light energy to generate photoacoustic signals, and uses ultrasonic detectors to receive and record different groups of photoacoustic signals (as shown in table 1); then calculate the ratio of the photoacoustic signal amplitude of two wavelengths under the same pH value, and count its error, depict the photoacoustic pH standard curve as image 3 shown.

[0040] Table 1 Photoacoustic signals of acid-base detection solutions with different pH values ​​under laser irradiation with a wavelength of 532nm

[0041]

[0042] ...

Embodiment 3

[0044] Use industrial wastewater as the sample to be tested, take a small amount of wastewater sample with a beaker, and cover the transparent film at the front end of the photoacoustic pH measuring probe in the sample. The laser outputs pulsed lasers with wavelengths of 532nm and 460nm respectively, and the laser output energy is determined by the pulse power meter. (Ophir Nova II) measurement, and ensure that the output energy of the two wavelengths is the same, and then record the photoacoustic signal before and after adding the photoacoustic acid-base indicator to the tested sample. The tested sample is also tested with the PH test pen (PHTestr 30) to monitor. Photoacoustic measurement results such as Figure 4 and Figure 5 as shown, Figure 4 It is the photoacoustic signal of industrial wastewater excited by a laser with a wavelength of 532nm, where the dotted line is the photoacoustic signal of pure industrial wastewater, and the solid line is the photoacoustic signal...

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Abstract

The invention provides an acousto-optical alkalinity-acidity imaging method and device based on development difference of reagents. The method comprises the steps of dissolving acid-base indicator in the straight alcohol solution to serve as acousto-optical alkalinity-acidity detection reagent; recording the intensity of the acousto-optical signals of the alkalinity-acidity detection reagent at different PH values by using the acousto-optical detection system, and drawing the acousto-optical alkalinity-acidity detection standard curve; dyeing the object to be detected by the alkalinity-acidity detection reagent, measuring the intensity of the acousto-optical signals of the object to be detected before and after dyeing, processing the acousto-optical image by calculating, determining the alkalinity-acidity of the object to be detected compared with the standard curve, and reconstructing the acousto-optical image expressing the alkalinity-acidity distribution by using a back projection algorism. The device implementing the method is composed of a laser emitting assembly, an ultrasonic detection assembly, a data acquisition and transmission assembly, a computer, and an image processing software. The method and the device have high sensitivity, and can be used for measuring not only the alkalinity-acidity of liquid substances, but also the alkalinity-acidity of semisolid and solid substances.

Description

technical field [0001] The invention relates to a pH measurement technology, in particular to a photoacoustic pH imaging method and device based on reagent color difference, and belongs to the field of detection technology. Background technique [0002] At present, most of the measurement methods for the pH of the solution are manual methods, using pH test paper or indicators for measurement, but the color change range of test paper and indicators is judged by human eyes, resulting in low accuracy of this measurement method , especially for the change of the pH value below 0.5, it is quite difficult to judge the color by human eyes. And some pH value measuring instruments with electrodes as sensitive parts, although the measurement of the pH value of the solution is more accurate, and the volume is small, easy to carry, but these measuring instruments cannot directly measure solid objects, such as isolated meat, fruits, soil etc. The measurement of pH value is of great sig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/80G01N21/63
Inventor 杨思华邢达
Owner SOUTH CHINA NORMAL UNIVERSITY
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