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Detecting method for evaluating tumor growth safety promotion of cell growth factor

A technology of growth factors and detection methods, which is applied in the field of detection for evaluating the safety of cell growth factors in promoting tumor growth, can solve problems such as inability to reflect biological characteristics, and achieve reliable experimental results

Inactive Publication Date: 2009-04-29
JINAN UNIVERSITY
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Problems solved by technology

However, due to the special biological characteristics and mechanism of action of genetically engineered drugs, traditional evaluation methods for this type of special biological products obviously cannot reflect their true biological characteristics. Special index system for new drug safety evaluation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0013] If the cell division and growth rate of the cell growth factor group were significantly higher than that of the control group; if the phosphorylation level of CREB protein, CREB, CBP, P300, ATF1-3, Elk1, GTF2b, Polr2a-c, Tbp of cells in the cell growth factor group The expression levels of genes or proteins such as ect. were significantly higher than those of the control group, while the expression levels of CREM and ICER were significantly lower than those of the control group. It can be preliminarily judged that the growth factor may excessively promote cell division and growth. On the contrary, it means that the drug does not promote cell division and growth. If the number of clones and tumor volume in the cell growth factor group are significantly greater than those in the control group, it can be preliminarily judged that the growth factor has the possibility of inducing tumorigenesis and growth.

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Abstract

The invention discloses a detection method for evaluating the safety of cell growth factor to promote tumor growth, which comprises the following steps: firstly, using a CFSE marker flow cytometry and a [<3>H] incorporation method to measure the speeds of cell division and cell growth respectively; secondly, measuring CREB passageway related genes and protein expression level, and measuring the phosphorylation level of a CREB protein, and the combination of CREB, CREM, ICER, CBP, P300 and a growth regulatory gene promoter; and finally, evaluating the clone forming number and the tumor volume. The method applies the molecular biology and the cell biological technology combining with in vitro and in vivo test results to evaluate the safety of the cell growth factor to promote the tumor growth. The method can also be used for evaluating the safety of other genetic engineering drugs.

Description

technical field [0001] The invention designs a detection method for evaluating the safety of cell growth factors promoting tumor growth. Background technique [0002] The development history of biopharmaceuticals, especially genetic engineering pharmaceuticals is relatively short. Judging from the development of the current technical level, the upstream and downstream technologies of genetic engineering pharmaceuticals, including plasmid components, fermentation processes, protein purification, and a series of related technologies have been basically solved and are relatively mature. At present, people are most concerned about the safety of these genetically engineered drugs on the human body, that is, the pharmacology and toxicology. Cell growth factors are a kind of regulatory factors that can promote cell growth and are closely related to the growth and development of tumors. Their safety is an important issue that people are very concerned about. At present, the evalua...

Claims

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Application Information

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IPC IPC(8): G01N33/48C12Q1/02
Inventor 姚成灿查振刚吴昊屠美
Owner JINAN UNIVERSITY
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