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Method for preparing mannanase by using glycerol as carbon source and fedbatch of substrate for induction

A technology of mannanase and substrate induction, applied in the direction of microorganism-based methods, biochemical equipment and methods, enzymes, etc., can solve the problems of unfavorable yeast growth, increase of production process and cost, low yield, etc., to achieve Effects of reducing metabolic repressors, reducing processing costs, and reducing production costs

Inactive Publication Date: 2009-04-22
LIAONING HUAXING BIOTECHOLOGY
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Problems solved by technology

Its disadvantages are: first, the caramelization reaction produces harmful substances, which is not conducive to the growth of yeast; second, the post-processing of the dark fermented liquid requires decolorization, which increases the production process and cost
Third, without adding the substrate mannan for induction, the yield is low

Method used

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  • Method for preparing mannanase by using glycerol as carbon source and fedbatch of substrate for induction
  • Method for preparing mannanase by using glycerol as carbon source and fedbatch of substrate for induction

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Embodiment Construction

[0012] A method for inducing mannanase by using glycerol as a carbon source and adding substrate mannan, using Pichia pastoris as a carrier for the production of mannanase by fermentation of genetically engineered bacteria, characterized in that glycerol is used as Carbon source and feed substrate mannan, the specific process is as follows:

[0013] 1. Culture medium

[0014] 1. Seed medium: 3% by weight of glucose, 2% of peptone, 2% of yeast powder, and the balance is water;

[0015] 2. Basal medium: 2%~10% by weight of glycerol, 1%~5% of potassium dihydrogen phosphate, 1%~5% of potassium sulfate, 1%~5% of phosphoric acid with a purity of 85%, and sulfuric acid heptahydrate Magnesium 1%~5%, the balance is water;

[0016] 2. Fermentation process

[0017] 1. Culture of bacteria

[0018] a. Take an appropriate amount of mannanase-producing strain culture slant for production, and insert them into 3 to 4 1000ml triangular flasks with 500ml seed culture medium inside;

[0019...

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Abstract

The invention relates to a preparation method for mannanase by induction of a substrate batch fed into a carbon source of glycerol, in particular to a fermentation production process for genetically engineered strain of the mannanase with Pichia stipitis as a carrier. The invention is characterized in that a seed culture solution is used for culture in a first-stage seed culture fermentor; a basic culture solution is used for culture in a second-stage seed fermentor; part of the basic culture solution is poured into a production fermentor for culture at initial time, and the glycerol, methanol and mannan substrate are respectively fed into a fermentation solution to finish the fermentation at a certain proportion and speed in the fermentation process. The yield of the mannanase can be obviously increased by 15 to 24 percent.

Description

technical field [0001] The invention relates to a fermentation technology of microbial products, a method for inducing and preparing mannanase by using glycerol as a carbon source and feeding substrates. Background technique [0002] The traditional method of producing mannanase uses glucose as a carbon source. Glucose, as a carbon source, produces caramelization during the high-temperature sterilization process, making the fermentation medium solution brown or soy sauce-colored. Its disadvantages are: first, the caramelization reaction produces harmful substances, which is not conducive to the growth of yeast; second, the post-processing of the dark fermented liquid requires decolorization, which increases the production process and costs. Third, the induction is performed without adding the substrate mannan, and the yield is low. Contents of the invention [0003] The purpose of the present invention is to provide a method for inducing and preparing mannanase by using ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/42C12R1/84
Inventor 韩国宝王武良
Owner LIAONING HUAXING BIOTECHOLOGY
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