Method of preparing micro-ecological complex bacterial agent
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A compound bacterial agent, micro-ecological technology, applied in the direction of medical preparations, bacteria, fungi, etc. containing active ingredients, to achieve the effect of promoting recovery, inhibiting growth and reproduction, and enhancing physical fitness
Inactive Publication Date: 2011-04-06
INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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Problems solved by technology
[0008] The purpose of the present invention is to solve the problem that the existing probiotics can only temporarily increase and supplement the number of probiotics in the human intestinal tract in a short period of time. Probiotics cannot survive "effectively" in the human intestinal tract, making it difficult for probiotics to form a scale, poor competitiveness, and poor viability. Often, the probiotics will disappear after a period of time. Method for the preparation of ecological preparations
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specific Embodiment approach 1
[0017] Specific embodiment 1: The micro-ecological compound bacterial agent in this embodiment is mainly composed of Bacillus natto, Kluyveromyces lactis, Lactobacillus acidophilus and soybean oligosaccharides; , Kluyveromyces lactis and Lactobacillus acidophilus total number of live bacteria is 1.0×10 10 ~9.9×10 10 The colony ratio of Bacillus natto, Kluyveromyces lactis and Lactobacillus acidophilus is 1~3:1~3:1~3; 10% to 30% of the total mass of the composite bacterial agent.
[0018] According to the acute toxicity test, it is proved that the microecological compound microbial agent of this embodiment has no toxic and side effects. The median lethal dose of the micro-ecological compound bacterial agent in this embodiment is above the maximum tolerated dose of 13 g / (kg·bw).
[0019] The micro-ecological compound bacterial agent of this embodiment can adjust the intestinal flora imbalance of mice caused by antibiotic-associated diarrhea, and has obvious antidiarrheal effe...
specific Embodiment approach 2
[0022] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the colony ratio of Bacillus natto, Kluyveromyces lactis and Lactobacillus acidophilus is 1:1:1. Others are the same as the first embodiment.
specific Embodiment approach 3
[0023] Specific embodiment three: the difference between this embodiment and specific embodiment one is: the micro-ecological compound bacterial agent also includes skimmed milk powder accounting for 1-30% of the total mass of the micro-ecological compound bacterial agent, 1-3% trehalose , 1-3% glycerin, 1-3% sucrose and 0.1-0.3% sodium glutamate. Others are the same as the first embodiment.
[0024] In this embodiment, the microecological compound bacterial agent can cure more than 98% of the diarrhea caused by the intestinal flora imbalance caused by the use of antibiotics. In this embodiment, skimmed milk powder, trehalose, glycerin, sucrose and sodium glutamate are dissolved in distilled water to make a protective agent, so that the mass concentration of skimmed milk powder in the protective agent is 10%, and the mass concentration of trehalose is 1 %, the mass concentration of glycerol is 1%, the mass concentration of sucrose is 1%, and the mass concentration of sodium g...
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Abstract
The invention relates to a method for preparing the micro-ecological composite fungus preparation, in particular to a method for preparing the micro-ecological preparation. The method solves the problems that the prior micro-ecological preparation can only temporarily increase and supplement the amount of probiotics in intestinal canal of a human body in a short period, and has poor competitiveness and viability so that the probiotics are used up after a period of time of stopping taking the micro-ecological preparation. The micro-ecological composite fungus preparation mainly comprises bacillus natto, lactic acid kluyveromyces, lactobacillus acidophilus and soybean oligosaccharides. The method comprises the following steps: culturing micro-ecological funguses; intermediately culturing the micro-ecological funguses; culturing and centrifuging the micro-ecological funguses after the micro-ecological funguses are transferred into a high concentration culture fluid; and adding auxiliary materials to the micro-ecological funguses, pre-freezing and vacuum freeze-drying the mixture, and adding the soybean oligosaccharides to the mixture to obtain the micro-ecological composite fungus preparation. The total number of viable beneficial bacteria in the micro-ecological composite fungus preparation is between 1.0 x 10<10> and 9.9 x 10<10> per gram, the micro-ecological composite fungus preparation not only has huge unit number, but also has high survival rate of the viable bacteria of more than 98 percent two years later.
Description
technical field [0001] The invention relates to a preparation method of probiotics. Background technique [0002] With the acceleration of the pace of life, the increase of food types and styles, as well as environmental pollution, indiscriminate use of antibiotics and other factors, the micro-ecological environment in the human intestinal tract is disturbed, resulting in the accumulation of toxins in the human body, causing constipation, diarrhea, hepatitis and other digestive tract diseases. It can even be life threatening. In addition to paying attention to dietary hygiene, balanced diet, and strengthening physical exercise, it is an ideal method to use microecological preparations to rebuild a healthy intestinal microecological environment. [0003] The number of live bacteria of probiotics in the current probiotics is small (all less than 300 million / gram), and the number of live bacteria of probiotics decreases with time after preparation (the number of live bacteria...
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