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Mass spectrum model used for detecting liver cancer characteristic protein and preparation method thereof

A characteristic protein and mass spectrometry model technology, which is applied in the field of malignant tumor liver cancer detection, achieves high sensitivity and specificity, reasonable and feasible design, and improves survival rate and quality of life

Inactive Publication Date: 2009-04-08
BIOYONG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the accuracy of MALDI-TOF is as high as 0.1%-0.01%, which is much higher than that of SDS electrophoresis and high-performance gel chromatography currently used routinely, there are still some defects in the application of tumor markers, especially liver cancer markers. Therefore, So far, there is no report on the detection of liver cancer markers or liver cancer serum characteristic proteins using MALDI-TOF-MS technology in China

Method used

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  • Mass spectrum model used for detecting liver cancer characteristic protein and preparation method thereof
  • Mass spectrum model used for detecting liver cancer characteristic protein and preparation method thereof
  • Mass spectrum model used for detecting liver cancer characteristic protein and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Establishment of Liver Cancer Mass Spectrometry Model

[0037] 1. Samples and instruments:

[0038] A total of 109 serum samples were collected in two batches. The first batch of samples was 79 cases, of which 49 cases were from liver cancer patients and the other 30 cases were from healthy people. This batch of 79 samples was used as the training group. The second batch of samples was 30 cases, including 20 cases of liver cancer patients and 10 cases from healthy people. This batch of 30 samples was used as the test group. All 69 patients with liver cancer were confirmed by postoperative pathology report. All serum samples were drawn in the morning on an empty stomach, and the serum was separated and stored in a -80 low-temperature refrigerator.

[0039] The matrix-assisted laser desorption time-of-flight mass spectrometer Autoflex II TOF / TOF and the WCX magnetic bead kit used in the experiment were developed by Bruker Company of the United States. The d...

Embodiment 2

[0057] Embodiment 2 detection of liver cancer

Embodiment 3

[0069] Comparison of Example 3 and other liver cancer detection methods

[0070] For the diagnosis of liver cancer, it is analyzed according to its biological characteristics, age, gender, disease location and clinical process. On the basis of medical history and signs, the diagnosis and treatment of liver cancer using AFP and ultrasound have made significant progress in general. However, there seems to be no breakthrough in the treatment of liver cancer, and most patients die soon after diagnosis. An earlier diagnosis method is urgently needed. However, the principle of the method of the present invention is different from the above detection methods, and the results of the method of the present invention show that the detection of liver cancer by using serum polypeptide is also a very effective diagnostic method, which is of great benefit to the diagnosis of liver cancer at an earlier stage.

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Abstract

The invention provides a mass spectrometry model used for detecting hepatocellular carcinoma serum characteristic protein, and a preparation method used for the mass spectrometry model. The method uses a plurality of characteristic protein combinations which are different in hepatocellular carcinoma patients and normal people to detects the hepatocellular carcinoma serum, adopts a method combining the traditional statistics with the modern biological informatics to process data, measures the protein combination spectrum spectrogram of serum samples of hepatocellular carcinoma patients and normal people by utilizing a mass spectrograph, and screens out the tumor markers of five corresponding hepatocellular carcinoma characteristic proteins, thus preparing the mass spectrometry model used for detecting the hepatocellular carcinoma serum characteristic proteins and provides foundation and resource for finding newer and more ideal tumor markers.

Description

technical field [0001] The invention relates to the field of malignant tumor liver cancer detection, and is a brand-new non-invasive detection method for early discovery and detection of liver cancer in vitro, and the sensitivity and specificity both reach 100%. Background technique [0002] Primary liver cancer (hereinafter referred to as liver cancer) is one of the high incidence cancers in my country. Not only the incidence rate is very high in the southeast coastal areas, but also the incidence rate in the northwest area is not low. The reason is roughly related to the widespread prevalence of hepatitis B in my country. Now hepatitis B vaccination has been gradually promoted in our country. According to preliminary reports, the incidence of liver cancer has been significantly reduced in adolescents who have received hepatitis B vaccination. However, the high incidence of liver cancer is in the middle-aged and elderly people, so it will take time for the incidence of l...

Claims

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Application Information

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IPC IPC(8): G01N33/48G01N27/64
Inventor 王岚殷汝雷马庆伟于中连胡晓慧吕萍萍
Owner BIOYONG TECH
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