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Quick-speed generation method of anaerobic microorganism culture environment

An anaerobic microorganism and fast technology, applied in the field of bioengineering, can solve the problems of equipment difficult to meet the requirements of anaerobic bacteria cultivation, difficult to popularize application, expensive price, etc., and achieve the effect of low price, lower cost and faster response speed

Inactive Publication Date: 2009-04-01
HANGZHOU BASEBIO BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] To sum up, the mature and reliable anaerobic microorganism cultivation equipment at home and abroad are expensive and costly, making it difficult to achieve universal application
However, it is difficult to meet the requirements of anaerobic culture with cheap and convenient equipment. Therefore, there is an urgent need for an anaerobic microbial culture method that can meet the conditions for anaerobic culture and can greatly reduce equipment investment costs.

Method used

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  • Quick-speed generation method of anaerobic microorganism culture environment
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  • Quick-speed generation method of anaerobic microorganism culture environment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Clostridium putrefaciens CVCC96 was inoculated into bacterial thioglycollate medium, and Azotobacter sphaeroides CICC 20025 was inoculated into potato dextrose agar medium. The two inoculated culture media were put into a well-sealed and transparent box with a volume of 5.6 L, and a resazurin test paper was built in the box as an indicator of the redox state. Quickly add oxygen scavenger components, carbon dioxide generator components, and hydrogen generator components to the three preset containers in the box, and seal the box body. The entire process time is controlled within 2 minutes.

[0037] The oxygen scavenger components used are: 100 ml of 2.3 mol / L pyrogallic acid solution, 100 ml of 6.7 mol / L sodium hydroxide solution. The components of the carbon dioxide generating agent are: 100 ml of 0.25 mol / L sodium bicarbonate solution, and 100 ml of 0.091 mol / L citric acid solution. The hydrogen generator components are: 0.0275mol sodium borohydride and 100ml water. ...

Embodiment 2

[0041]Example 1 was repeated in the same manner as described, but the oxygen scavenger auxiliary was simultaneously added to the vessel containing the oxygen scavenger component. The oxygen scavenger components used are: 100ml of 2.1mol / L pyrogallic acid solution, 100ml of 13.5mol / L sodium hydroxide solution, and the oxygen scavenger auxiliary agents are: reduced iron powder 0.0714mol, sodium chloride 0.025mol, activated carbon 0.1667 mol, carbon dioxide generating agent components are: 0.125mol / L sodium bicarbonate solution 100ml, 0.0455mol / L citric acid solution 100ml, hydrogen generating agent components are: 0.04mol sodium borohydride and 100ml water.

[0042] Through the determination of the oxygen concentration and carbon dioxide concentration in the box, the relationship between carbon dioxide concentration, residual oxygen concentration and time is established, see the attached figure 2 .

[0043] After 24 hours of constant temperature cultivation at 37°C, the result...

Embodiment 3

[0045] Example 1 was repeated in the same manner as described, but the oxygen scavenger auxiliary was simultaneously added to the vessel containing the oxygen scavenger component. The oxygen scavenger components used are: 100ml of 2.8mol / L pyrogallic acid solution, 100ml of 2.1mol / L sodium hydroxide solution, and the oxygen scavenger auxiliary agents are: reduced iron powder 0.0728mol, activated carbon 0.14mol, carbon dioxide generator components It is: 100ml of 0.2mol / L sodium bicarbonate solution, 100ml of 0.0728mol / L citric acid solution, and the hydrogen generator components are: 0.028mol sodium borohydride and 100ml water.

[0046] Through the determination of the oxygen concentration and carbon dioxide concentration in the box, the relationship between carbon dioxide concentration, residual oxygen concentration and time is established, see the attached image 3 .

[0047] After 24 hours of constant temperature cultivation at 37°C, the results of bacterial cell culture w...

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Abstract

The invention provides a fast generation method of anaerobe culture environment with convenience, fastness, high efficiency and low cost, which adds a deoxidant, a carbon dioxide propellant and a hydrogen propellant in a sealed case so as to remove the oxygen in the case, generate carbon dioxide and hydrogen with a proper proportion, and achieve the purpose of maintaining the pressure balance in the case through the cooperative interaction of the deoxidant, the carbon dioxide propellant and the hydrogen propellant; the whole phenomenon is observed through the color change of an indicator. Theinvention also provides a combined reagent for realizing the fast generation method of anaerobe culture environment, which consists of the deoxidant, the carbon dioxide propellant and the hydrogen propellant.

Description

technical field [0001] The invention relates to a method for quickly generating an anaerobic microorganism culture environment, which belongs to the technical field of bioengineering. Background technique [0002] Anaerobic bacteria (anaerobic bacteria) is a class of bacteria that can only grow under the condition of low oxygen partial pressure, but cannot grow on the surface of solid medium in air (21% oxygen) and higher than 10% carbon dioxide concentration. According to their tolerance to oxygen, they can be divided into obligate anaerobes, microaerophilic anaerobes and facultative anaerobes. This type of bacteria lacks a complete metabolic enzyme system, and its energy metabolism is carried out by anaerobic fermentation. [0003] Anaerobic microorganisms are very harmful, and they can cause infections in different parts of the human body; in food microbiology, people's awareness of the hazards of anaerobic microorganisms to food pollution is also gradually increasing, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
Inventor 姚毓奇
Owner HANGZHOU BASEBIO BIO TECH
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