Optical fiber bio probe for directionally fixing antibody and method for making same

A bioprobe and directional immobilization technology, applied in the field of optical fiber bioprobe and its preparation, can solve the problems of easy inactivation of antibody molecules, large steric hindrance of antibody molecules, unfavorable antibody-antigen binding, etc., to reduce non-specific adsorption , The effect of improving the analytical sensitivity

Inactive Publication Date: 2009-03-18
CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to overcome the above-mentioned steric hindrance of the antibody molecule directly immobilized on the solid phase surface, the functional domain of the antibody molecule tends to go down or to the side on the solid phase surface, and the antibody molecule binds to the antigen. The functional domain is adsorbed on the solid phase surface, which is not conducive to the antibody-antigen combination, and the shortcomings of antibody molecules are easy to inactivate. In order to improve the binding ability of the antibody and antigen on the optical fiber biological probe, a fiber optic biological probe with fixed antibody is provided. Needle and its preparation method

Method used

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  • Optical fiber bio probe for directionally fixing antibody and method for making same
  • Optical fiber bio probe for directionally fixing antibody and method for making same
  • Optical fiber bio probe for directionally fixing antibody and method for making same

Examples

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Effect test

Embodiment 1

[0038] Prepare the optical fiber bioprobe that directionally immobilizes anti-BSA on the surface of the optical fiber, and its structure includes: such as image 3 As shown, the optical fiber 1, the hydrophilic polarization layer 2 forming a monomolecular layer on the surface of the optical fiber, the protein G monomolecular layer 3 formed on the hydrophilic polarization layer 2, and the protein G molecular layer 3 formed on the Capture layer 4 of anti-BSA antibody molecules.

[0039] The preparation of the optical fiber bioprobe is carried out in the following steps:

[0040] 1. Cleaning treatment of optical fiber surface

[0041] Surface clean it with conventional cleaning methods.

[0042] 2. Formation of hydrophilic polarized layer

[0043] Clean the optical fiber core cleaned in the above step 1 in the cleaning solution 1 for 30 minutes, then in the cleaning solution 2 for 30 minutes, and then boil it in the cleaning solution 3 for 10 minutes to prepare the fiber core ...

Embodiment 2

[0054] Prepare a fiber-optic bioprobe on the surface of the fiber-optic core that is oriented and fixed against HbsAg. The protein G monomolecular layer 3 formed on the polarized layer 2, and the anti-HbsAg capture antibody molecular layer 4 formed on the protein G molecular layer 3.

[0055] Adopt the step in embodiment 1 to obtain protein G molecular layer, capture the preparation of molecular layer,

[0056] Immerse the protein G molecular layer formed in the above step 3 into the anti-HbsAg solution with a concentration of 1 mg / mL, soak for 30 minutes, take it out and wash it with deionized water, and obtain the capture antibody molecule anti-HbsAg on the protein G molecular layer Molecular layer, so as to make a fiber-optic bioprobe directional immobilized anti-HbsAg on the surface of the fiber-optic core, its structure is as follows image 3 shown.

[0057] Directional fixed anti-HbsAg fiber optic bioprobe for detection of BSA:

[0058] Immerse all the fiber optic bio...

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Abstract

The invention relates to an optical fiber biological probe for the evanescent wave optical fiber biological sensor in immunity test, in particular to an optical fiber biological probe which surface is directionally fixed with antibodies and a preparation method thereof. The biological probe is composed of an optical fiber and a capture antibody molecule layer fixed on the surface of the optical fiber, which is characterized in that a protein G molecule layer is arranged between the optical fiber and the capture antibody molecule layer. The preparation method comprises: immersing an clean optical fiber into a protein G solution, to obtain the saturated adsorption layer of the protein G, immersing into the capture antibody molecule solution, to combine the capture antibody molecules via the proteins G until saturation, to obtain the directional fixed antibody optical fiber biological probe. The optical fiber biological probe overcomes the defect such as reduced biological activity of the antibody caused by directly fixing antibodies on the surface of the fiber core, to reduce the spatial resistance of the antibody molecules, effectively reduces the non-specific adsorption of the surface, and improves the analysis sensitivity of the detection antigens.

Description

technical field [0001] The invention relates to an optical fiber bioprobe on an evanescent wave optical fiber biosensor used for immunological detection, in particular to an optical fiber bioprobe for directionally immobilizing antibodies on the surface of an optical fiber and a preparation method thereof. Background technique [0002] At present, the widely used biological detection system based on the optical fiber evanescent wave biosensor is to use the evanescent wave generated during the transmission of light waves in the optical fiber in the form of total reflection to excite and bind to the biomolecules on the surface of the optical fiber probe through a bioaffinity reaction. labeled fluorescent dyes. A fiber optic probe immobilized on the surface to capture biomolecules is placed in the sample to be detected. If there is the same biological substance in the sample as the biomolecules immobilized on the surface of the fiber optic probe, the two will undergo an affinit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N21/64
Inventor 孔祥贵张友林曾庆辉孙雅娟刘晓敏
Owner CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI
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