Application of cisplatin combined with hTERT-HRP/IAA in medicament for treating cervical cancer cell

Abstract

The invention discloses an application of cisplatin combined with phTERTp-HRP / IAA in drugs for the treatment of cervical cancer cells, in particular to the strengthening role of the cisplatin on the activity of a hTERT gene promoter. The cisplatin and the phTERTp-HRP / IAA are commonly used in the synergy for killing the human cervical cancer cells, and the action mechanism thereof is further explored. The application adopts Hela cells for carrying out the study and evaluates the impacts of the cisplatin on the activity of the hTERT promoter through the expression of downstream luciferase reporter gene regulated by the hTERT promoter. The synergic killing role and the mechanism of the cisplatin and the hTERT promoter during the combined use for regulating the gene therapy of an HRP / IAA system are evaluated through the detection of cell proliferation rate, cell apoptosis rate and cell cycle. The results confirm that the cisplatin can strengthen the activity of the hTERT gene promoter; and when in combined use with the phTERTp-HRP / IAA, the killing role of the human cervical cancer cells is significant.

Description

Technical field The present invention relates to the technical field of medicine, and more specifically to the application of cisplatin in drugs that enhance the gene therapy mediated by the telomerase reverse transcriptase gene (hTERT) promoter. Cisplatin enhances the activity of the hTERT promoter and enhances downstream gene expression In the role. At the same time, it also relates to the application of cisplatin and hTERT promoter to regulate horseradish peroxidase (HRP) / indole acetic acid (IAA) system to produce a synergistic effect to destroy cervical cancer cells and inhibit the growth of cervical cancer cells. Background technique Looking at the pattern of disease spectrum changes at home and abroad, malignant tumors have become a major disease that seriously threatens human health. With the advancement of molecular biology, gene therapy has brought hope to overcome tumors. Many countries have carried out a large number of studies on gene therapy of malignant tumors and h...

AI Technical Summary

Problems solved by technology

It was found that the combined use of cisplatin or carmustine at the half inhibitory rate (IC50) of them can reduce the activity of telomerase in the cells, resulting in a decrease in the combined killing effect

Images