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Virus-free in situ and alcohol fermentation method of composite bacteria for lignocellulose hydrolysis product

A lignocellulose and ethanol fermentation technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of ethanol fermentation process complexity and cost increase, and achieve low cost, simple equipment requirements, and long fermentation time The effect of shortening

Inactive Publication Date: 2010-11-10
CAPITAL NORMAL UNIVERSITY
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Problems solved by technology

The hydrolyzate is usually detoxified before fermentation, which requires the addition of treatment equipment, and the detoxification equipment must be well integrated with the fermentation process, thus increasing the complexity of the entire ethanol fermentation process and increasing the production cost

Method used

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  • Virus-free in situ and alcohol fermentation method of composite bacteria for lignocellulose hydrolysis product
  • Virus-free in situ and alcohol fermentation method of composite bacteria for lignocellulose hydrolysis product
  • Virus-free in situ and alcohol fermentation method of composite bacteria for lignocellulose hydrolysis product

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Embodiment 1

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Abstract

The present invention relates to an in-situ detoxification ethanol fermentation method of lignocellulose hydrolysate, which respectively inoculates Y5 and CBS6054 into fermentation culture medium containing dilute acid lignocellulose hydrolysate to carry out in-situ detoxification alcoholic fermentation. The method can efficiently metabolize glucose and xylose in the dilute acid lignocellulose hydrolysate to produce ethanol, and rapidly metabolize furfural and 5-hydroxymethylfurfural. The yield of ethanol is 0.44g / g, reaching 87.5 percent of the theoretical value. Moreover, any detoxificationprocessing is not needed, the requirement on equipment is low, the operation is convenient, and the cost is low.

Description

In situ detoxification ethanol fermentation method of lignocellulose hydrolyzate by complex strains technical field The invention relates to an in-situ detoxification ethanol fermentation method of lignocellulose hydrolyzate, in particular, a method that can efficiently metabolize glucose and xylose in dilute acid hydrolyzate of lignocellulose to produce ethanol and rapidly metabolize inhibitory factor The complex bacteria of furfural and 5-hydroxymethylfurfural were inoculated into the fermentation medium containing lignocellulose dilute acid hydrolyzate for in-situ detoxification ethanol fermentation. Background technique In the field of research and development of ethanol production using lignocellulose as raw material, dilute acid hydrolysis of lignocellulose is considered to be the most easily commercialized production process, and a lot of in-depth research has been carried out in this field at home and abroad. But so far, there are still some problems in dilute acid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12P7/10C12R1/865C12R1/84
CPCY02E50/16Y02E50/10
Inventor 杨秀山
Owner CAPITAL NORMAL UNIVERSITY
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