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Extraction method of polysaccharides from morinda officinalis how and application thereof in cigarette

A technology of Morinda officinalis and polysaccharides, which is applied in the extraction method and the application field in cigarettes, can solve the problems that the extraction and purification of Morinda officinalis polysaccharides has not been reported and has not been considered, and can improve the quality of smoking, improve the taste, improve the The effect of smoking quality

Inactive Publication Date: 2010-12-08
LONGYAN CIGARETTE FACTORY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the influence of different process conditions, such as temperature, solid-liquid ratio, extraction time and concentration ratio on the yield of polysaccharides, has not been considered.
Hydrogen peroxide, as an oxidative decolorizing agent, has been reported for the extraction and purification of plant polysaccharides, but there is no relevant report for the extraction and purification of Morinda officinalis polysaccharides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] a. Dry the Morinda officinalis officinalis after washing, removing impurities, removing the wood core in the middle of the particles, drying or sun-drying at 60°C, and then crush it to 40 mesh;

[0021] B, get the Morinda officinalis powder obtained in step a, according to water:material (m / m)=20:1, carry out leaching extraction under the condition of 70 ℃, leaching time is 2h;

[0022] c, filter and concentrate the filtrate to 1 / 6 of the volume of the original filtrate;

[0023] d. Add the concentrated solution into absolute ethanol, keep the volume fraction of ethanol at 80%, set aside for 48 hours after mixing, and obtain the crude polysaccharide of Morinda officinalis officinalis after filtering, with a yield of 13.8%

[0024] e, the obtained Morinda officinalis crude polysaccharide of d step is put into water and is redissolved, is mixed with the solution that mass concentration is 5%, then adds the sevag reagent [chloroform: n-butanol (V:V] that volume is 0.8 time...

Embodiment 2

[0029] a. Dry the Morinda officinalis officinalis after washing, removing impurities, removing the wood core in the middle of the particles, drying or sun-drying at 60°C, and then crush it to 40 mesh;

[0030] B, get the Morinda officinalis powder obtained in step a, according to water:material (m / m)=40:1, carry out leaching extraction under the condition of 75 ℃, the leaching time is 1.5h;

[0031] c, filter and concentrate the filtrate to 1 / 10 of the volume of the original filtrate;

[0032] d. Add the concentrated solution to absolute ethanol, keep the volume fraction of ethanol at 80%, set aside to settle for 48 hours after mixing, and obtain the crude polysaccharide of Morinda officinalis after filtering, with a yield of 13.3%

[0033] e, the obtained Morinda officinalis crude polysaccharide of d step is put into water and redissolved, is mixed with the solution that mass concentration is 5%, then adds the sevag reagent [chloroform: n-butanol (V / V) that volume is 0.7 time...

Embodiment 3

[0038] a. Dry the Morinda officinalis officinalis after washing, removing impurities, removing the wood core in the middle of the particles, drying or sun-drying at 60°C, and then crush it to 40 mesh;

[0039] B, get the Morinda officinalis powder obtained in step a, according to water:material=25:1, carry out leaching extraction under 65 ℃ of conditions, leaching time is 1h;

[0040] C, filter, concentrate filtrate to 1 / 8 of former filtrate volume;

[0041] d. Add the concentrated solution to absolute ethanol, keep the volume fraction of ethanol at 80%, set aside for 48 hours after mixing, and obtain the crude polysaccharide of Morinda officinalis after filtering, with a yield of 12.9%;

[0042] e, the obtained Morinda officinalis crude polysaccharide of step d is put into water and is redissolved, is mixed with mass concentration and is 5% solution, then adds the sevag reagent [chloroform: n-butanol (V:V) that volume is 0.7 times of crude polysaccharide solution =4:1], shak...

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PUM

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Abstract

The invention provides a method for extracting polysaccharides from morinda root and the application of the polysaccharides in cigarettes. The method for extracting the polysaccharides comprises the steps as follows: morinda root is dried, and pulverized into powder of 10 to 100 meshes; the morinda root powder is immersed and extracted by adding water at 50 to 100 DEG C; subsequently, the mixtureof the morinda root powder and water is filtered and the filtrate is concentrated; the concentrated filtrate is added to ethanol to ensure the ethanol to account for 80 percent in volume; the mixtureof the concentrated filtrate and the ethanol are allowed to be settled out and filtered, thus getting the raw polysaccharides; the raw polysaccharides are put in water for redissolution; a sevag reagent 0.1 to 1 time of the raw polysaccharide solution in volume is added, and is shaken and allowed to be separated into layers; the mixture of the thick sevag and protein on the lower layer is removed; the pH value is adjusted to 9 to 10 with ammonia; oxydol 30 percent of the solution in mass is added slowly until the solution is turned into straw yellow; after the solution is decolored, the solution is condensed before ethanol with the concentration from 95 to 100 percent is added, the volume percentage of the ethanol is kept to be 80 percent; the solution is allowed to be settled out for 24 to 48 hours, thus getting the polysaccharides from morinda root through filtration.

Description

technical field [0001] The present invention relates to the extraction of plant polysaccharide and its application, in particular to the extraction method of Morinda officinalis polysaccharide and its application in cigarettes. The application of this technology does not change the existing processing technology of cigarettes, can improve the sensory quality of cigarettes, and reduce the harmfulness of cigarettes. Background technique [0002] As the problems of smoking and health attract more and more attention, it is an inevitable development trend of cigarettes to improve the smoking safety of cigarettes, increase health care functions, and reduce harmful components in cigarette smoke. Therefore cigarette varieties such as ginseng cigarettes and peppermint cigarettes have appeared on the market, that is, materials with unique functions or tastes such as ginseng and peppermint have been added in shredded tobacco. [0003] Morinda officinalis is a famous and precious tradi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A24B15/40A24B3/12
Inventor 范坚强杨振民黄华赵明月胡军刘加增伊勇涛余志强洪祖灿包可翔张峰胡有持
Owner LONGYAN CIGARETTE FACTORY
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