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Extraction method of antioxidative active Cordyceps sinensis polysaccharide

A technology of antioxidant activity and Cordyceps polysaccharides, applied in the direction of microorganism-based methods, biochemical equipment and methods, anti-toxins, etc., can solve problems such as loss, pollution of the environment, waste of resources, etc., to improve utilization rate, increase economic benefits, Take full advantage of the effect

Inactive Publication Date: 2009-01-28
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the current industrial production, the fermentation waste liquid of Cordyceps sinensis is generally discarded as a by-product, which not only pollutes the environment, but also wastes resources, resulting in a large loss of Cordyceps polysaccharide, the main biologically active substance of Cordyceps sinensis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: the acquisition of Cordyceps fermentation waste liquid

[0023] The fermentation strain is the Cordyceps fungus Paecilonyces hepialid chen&DaiCs-4. The seed medium was prepared as follows: glucose 1.5g, peptone 2g, inorganic salt 0.5g, water 100mL, pH7.0;

[0024] The fermentation medium was prepared as follows: 2g sucrose, 6g soybean meal powder, 2g peptone, 0.5g inorganic salt, 100mL water, pH7.2;

[0025] Put 150mL of seed culture medium in a 500mL triangular flask, insert the slant bacteria after sterilization, and culture in a shaking table with a rotation speed of 120r / min and 25°C for 3 days to obtain a seed liquid; the seed liquid is inserted into the seeds at a volume ratio of 5% inoculum tank, gradually enlarged to 20m 3 General-purpose stirred fermenter, 25 ℃, culture 5d. After the fermentation is finished, filter with a plate and frame filter press to remove the mycelium and obtain the Cordyceps fermentation waste liquid (total sugar content...

Embodiment 2

[0026] Embodiment 2: Extraction of Cordyceps polysaccharide

[0027] Take 1000 mL of Cordyceps fermented waste liquid prepared according to the method in Example 1 and concentrate to 42 mL. The concentrated liquid is a dark brown viscous liquid with a pH value of 1.5, a density of 1.304 g / mL, and a solid content of 51.33%.

[0028] Weigh 20g of the concentrated solution, dilute it with 50mL of distilled water, centrifuge at 4500r / min for 10min, take the supernatant, adjust the pH value of the supernatant to 4.88, add 4 times the volume of absolute ethanol to precipitate for 20min, and centrifuge at 4500r / min for 15min , collect the precipitate, wash the precipitate with 10 mL of absolute ethanol, 10 mL of acetone, and 10 mL of ether in sequence, and finally dry it at about 60 ° C to obtain 3.57 g of light brown crude polysaccharide powder, with an extraction rate of 7.75%.

[0029] Impurities are then further removed. Take 2.0 g of crude polysaccharide powder and re-dissolve ...

Embodiment 3

[0032] 1. Determination of the alcohol precipitation process based on the extraction rate:

[0033] According to the design principle of the Box-Behnken central combination experiment and the results of the single factor experiment, three factors that have a significant impact on the yield of polysaccharides, ethanol amount, alcohol precipitation time and pH value, were selected, and three factors and three factors were used on the basis of the single factor experiment. Horizontal response surface analysis method. The experimental analysis scheme is shown in Table 1:

[0034] Table 1

[0035] Test No.

[0036]After design-Expert software analysis, 22 groups of optimization schemes were listed. Considering comprehensively, the ethanol volume was selected as 4 times the supernatant volume, the alcohol precipitation time was 20min, and the pH value was 4.88. The theoretical extraction rate was 7.80%. After three repeated tests of this scheme, the extraction rates were...

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Abstract

The invention provides an extracting method of an antioxidation active cordyceps polysaccharide, in particular to a method for extracting the antioxidation active cordyceps polysaccharide by taking the waste fermentation liquid of Paecilonyces hepialid chen and amp; Dai Cs-e as materials. The method includes the steos that: liquid containing the antioxidation active cordyceps polysaccharide is used as the materials which are centrifuged for obtaining supernatant; the pH of the supernatant is adjusted to be 4.5 to 5.0; ethanol of 50 to 100 percent (V / V) with a volume of 2 to 5 times is added, deposition is carried out for 20 to 60 min, then centrifugation is carried out; a precipitate is taken and washed in sequence by ethanol, acetone and ether, then dried for obtaining the rough polysaccharide powder containing the cordyceps polysaccharide.

Description

(1) Technical field [0001] The invention relates to a method for extracting anti-oxidative active Cordyceps polysaccharides, in particular to a method for extracting anti-oxidative active Cordyceps polysaccharides from the fermentation waste liquid of the Cordyceps fungus Paecilonyces hepialid chen&Dai Cs-4. (2) Background technology [0002] More than 350 species of Cordyceps fungi have been found in the world, of which more than 70 species have been recorded in my country. Wild Cordyceps sinensis is mainly produced in Sichuan, Tibet, Yunnan, Qinghai and other cold areas above 3000m above sea level. Due to the limitation of its growth conditions and over-harvesting, the yield is very low, so the price is expensive. In recent years, as people gradually discover and understand the nourishing efficacy of Cordyceps and improve the immune function of the human body, the development and utilization of Cordyceps has attracted great attention from the world, and the research on Cor...

Claims

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Application Information

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IPC IPC(8): C08B37/00C12P19/04A61K36/068A61P39/06A61P37/02A23L1/09A23L1/29A23L1/28C12R1/79A23L29/30
Inventor 王普何军邀郑明李国权吴力平
Owner ZHEJIANG UNIV OF TECH
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