Method for removing larva toxin of masson pine caterpillar

A technology of masson pine and larvae, applied in the fields of application, food preparation, food science, etc., to achieve the effect of low cost, easy operation, and simple removal process

Inactive Publication Date: 2011-08-17
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above research provides a certain theoretical basis for the technology of the present invention. Through repeated explorations, we have obtained a simple and practical method for removing the toxin from Pinus massoniana caterpillars. At present, there is no relevant report at home and abroad.

Method used

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  • Method for removing larva toxin of masson pine caterpillar

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Take 5kg masson pine larvae and freeze them at -10°C for 5 hours, take them out, add 30L of 0.8% dilute salt solution, and soak them at room temperature (25-30°C) for 4 hours. The soaked larvae are obtained by filtration, and the filtrate can be used to develop insect toxin drugs. The soaked larvae are further fully treated at 70°C for 7 hours. Detection of toxin toxicity: using the conventional mouse acute toxicity test method, the LD was measured by oral administration of drugs to mice several times within 1 day 50 It is 11,960mg / kg (see Table 1), which belongs to the non-toxic standard.

[0013] Table 1 Acute toxicity test results of treated pine caterpillar larvae in mice

[0014]

[0015] (The actual maximum dosage is 11,960mg / kg)

Embodiment 2

[0017] Take 5kg masson pine larvae and freeze them at -10°C for 5 hours, take them out, add 35L of 1.0% dilute salt solution, and soak them at room temperature (25-30°C) for 5 hours. The soaked larvae are obtained by filtration, and the filtrate can be used to develop insect toxin drugs. The soaked larvae are further fully treated at 75°C for 7 hours. Detection of toxin toxicity: using the conventional mouse acute toxicity test method, the LD was measured by oral administration of drugs to mice several times within 1 day 50 It is 11,960mg / kg (see Table 1), which belongs to the non-toxic standard.

Embodiment 3

[0019] Take 5 kg of masson pine larvae and place them in a closed container, inject ether to kill them, take them out, add 40 L of 1.2% dilute salt solution, and soak them at room temperature (25-30° C.) for 4 hours. The soaked larvae are obtained by filtration, and the filtrate can be used to develop insect toxin drugs. The soaked larvae can be further fully treated at 80° C. for 7 hours. Detection of toxin toxicity: using the conventional mouse acute toxicity test method, the LD was measured by oral administration of drugs to mice several times within 1 day 50 It is 11,960mg / kg (see Table 1), which belongs to the non-toxic standard.

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Abstract

The invention discloses a method for wiping off toxin of dendrolimus punctatus larva. After larva is executed, the larva is dipped for 2-5 hours in 0.5-2% of borate solution; and then the larva is collected by filter; the larva is treated for 5-7 hours at the temperature of 70-85 DEG C. A mouse acute toxicity experience shows that the treated larva of dendrolimus meets non-toxicity standard. The method has simple removal technique, easy operation and low cost and lays the foundation for the development and utilization of dendrolimus living resources.

Description

technical field [0001] The invention belongs to the field of biological resource processing, and in particular relates to a method for removing toxins from pine caterpillar larvae. Background technique [0002] Pine caterpillar (Dendrolimus spp.) is the most serious forest pest in my country, and its annual direct economic loss is more than 1 billion yuan. Since the 1970s, the occurrence area of ​​pine caterpillars in my country is about 1.5-2 million hm per year 2 , The amount of biological resources is very huge. According to reports, only Zhejiang Province can capture about 1.4 million kg of pine caterpillars every year. However, from the perspective of resource science, pine caterpillars are a class of available biological resources. Pine caterpillars are rich in high-quality active proteins, and also contain relatively high levels of chitin and chitosan. Pine caterpillar oil is also a resource with potential application value, in which the content of polyunsaturated f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L1/015A23L5/20
Inventor 刘高强魏美才王晓玲牛耕耘
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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