Detection method for evaluating curative effect to treat angina pectoris by grisounites series drug treatment

Abstract

The invention relates to a detecting method for evaluating the curative effect of nitroglycerin and the similar medicament which treat angina pectoris, and the method comprises utilizing a sampling mop to scrap at least 40-60 times from top to bottom on the left and the right cheeks in the mouth of a measured person, gathering enough cell samples, extracting genome DNA, detecting the variation of mitochondria acetaldehyde dehydrogenase gene ALDH2 Glu504Lys, and evaluating the curative effect of nitroglycerin and the similar medicament which treat angina pectoris through detecting and analyzing the Glu504Lys site genotype on an individual ALDH2 gene. The invention has the advantages that the detecting method can be used to evaluate the validity of nitroglycerin and the similar medicament which treat angina pectoris.

Description

technical field [0001] The invention relates to a method for evaluating the curative effect of nitroglycerin drugs, which evaluates the effectiveness of nitroglycerin drugs for treating angina pectoris by detecting and analyzing whether the individual mitochondrial acetaldehyde dehydrogenase gene ALDH2 has Glu504Lys variation, which belongs to drug detection technology field. Background technique [0002] Angina pectoris is a clinical syndrome caused by coronary insufficiency, myocardial acute, temporary ischemia and hypoxia. [0003] Nitroglycerin (Glyceryl trinitrate, GTN) has been a classic drug for treating angina pectoris for more than 130 years. Sublingual administration of nitroglycerin has a quick effect (the drug directly enters the blood, and the symptoms are generally relieved within 5 to 10 minutes). And it can avoid the first-pass effect of the liver (that is, the liver degrades most of the drugs absorbed through the gastrointestinal tract), which is the standa...

AI Technical Summary

Problems solved by technology

Li Yifeng et al. found that in the process of GTN drug metabolism, heterozygous and homozygous mutant ALDH2 had higher Km values ​​and significantly lower Vmax values ​​than wild-type ALDH2, and the catalytic efficiency of ALDH2Glu504 enzyme was 10 times higher than that of Lys504, suggesting that This Lys504 polymorphism can affect the enzymatic activity of ALDH2 and reduce the biotransformation ability of GTN

[0007] In summary, mitochondrial acetaldehyde dehydrogenase Glu504Lys can affect the transformation of the biological activity of nitroglycerin, and then affect the efficacy of nitroglycerin drugs in the treatment of angina pectoris

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