ITS sequence of Tremella aurantiablba Bandoni and Stereum hirsutum and method for identifying golden fungus using the same

Abstract

The invention relates to a DNA sequence of a fungal strain and a method for identifying variety by means of the sequence. An ITS zone sequence of tremella aurantiabla is shown in SEQ ID NO.1, and an ITS zone sequence of host fungus stereum hirsutum is shown in SEQ ID NO.2. During identification, an ITS zone sequence of a to-be-identified sample is extracted at first and then is respectively compared with the SEQ ID NO.1 sequence and the SEQ ID NO.2 sequence to obtain a result. The method can accurately identify the authenticity of a to-be-identified fungus, and can also carry out accurate identification to artificially cultured yeast-like spore strain of tremella aurantiabla and host fungus stereum hirsutum strain; moreover, the method can accurately distinguish and identify the authenticity and purity of tremella aurantiabla variety or strain, thereby providing a reliable technique for variety identification, protection, strain quality control and effective strain breeding.

Description

The Sequence Group of ITS Regions of Auricularia aureus and P. pilosa and Its Identification Method of Auricularia technical field The present invention relates to a DNA sequence of a fungus variety and a method for identifying its variety by using the sequence. More specifically, the present invention relates to the sequence of the ITS region of the fungus Auricularia auricosa and a method for identifying its variety by using the sequence, which belongs to molecular biology methods The technical field of identification of fungal species and detection of fungal purity. Background technique Tremella aurantial ba Bandoniet Zang is a precious edible and medicinal fungus of the genus Tremella. It is rich in nutrients and can provide a variety of amino acids, vitamins and mineral elements necessary for the human body. It is a good biological nutrient resource. Golden ear has a long history of medicinal use, and its shape, medicinal parts and properties, flavors and functions ar...

AI Technical Summary

Problems solved by technology

The sources of commercially available golden fungus are complex, involving species with similar colors and shapes such as Tremellaaurantia Schw.exFr., Tremella encephala Pers., Tremellamesenterica Retzex Fr. or Tremellalutescens Fr., and artificially dyed tremella directly pretending to be golden ear, directly affects the clinical efficacy of golden ear and the interests of consumers

The current identification methods of golden ear include trait identification and microscopic anatomy, but these methods have not yet made a comprehensive and objective identification of the golden ear species