Reporter plasmid for biological detection of radioactive ray dose and construction method thereof

Abstract

The invention relates to a reporter plasmid in radiation dose used for biological detection and a preparation method thereof. The preparation method comprises the following steps: a shuttle vector between radiation resistant bacteria and coliform bacteria is taken as a foundation so as to be recombined with a DNA fragment in the luciferase gene lux field derived from the host DNA of a North American fire fly, thereby forming a reporter plasmid of the shuttle vector which has the activity of luciferase genes and is positioned between the radiation resistant bacteria and the coliform bacteria; the reporter plasmid is fed into a wild type R1 for transforming the radiation resistant bacteria so as to obtain transformant radiation resistant bacteria which are irradiated by gamma ray; the transformant radiation resistant bacteria are cultured to reach a stationary phase, and are irradiated by gamma rays so as to be cultured again; and according to the luminous quantity of a character transformant, different luminous quantities irradiated by the gamma rays in different doses are measured. Research result shows that within the adopted dose range of between 0 and 2, 000Gy, the correlation coefficient between the luminous quantities of thalli irradiated by the gamma rays in different luminous quantities and irradiation doses is equal to 0.892, reaching the extremely significant level.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a gene plasmid and a construction method thereof, that is, a reporter plasmid for biological detection of radiation dose and a construction method thereof. Background technique [0002] The health hazards of ionizing radiation and radiation safety assessment, the classification, diagnosis and treatment of acute and chronic radiation injuries under various conditions all need to understand and determine the exposure dose. The biological detection of radiation dose is the use of biological materials such as tissues, cells, DNA, proteins, etc. after ionizing radiation, which has a certain amount-effect relationship with the radiation dose. A class of biomarkers and analytical methods used to measure radiation doses through biological changes and analysis. The greatest advantage of using living organisms for dosimetry compared to physical detection is directness and faithful representatio...

AI Technical Summary

Problems solved by technology

[0003] At present, there are many methods for biological detection of radiation dose. Commonly used are: the relationship between the comet tail formed by DNA damage after radiation exposure and the comet core expressed by undamaged cells to study single-cell gel electrophoresis for predicting radiation dose (Comet test) method (Berwick and Vineins, 2000); there is also a chromosomal aberration analysis method (Ballarini and Ottolenghi, 2003; Duran et al., 2002; Camparoto et al., 2003), but the common disadvantages of these methods are complex operation, high detection cost, more human factors in damage observation, and difficult to quantify accurately, etc. Disadvantages: Although the use of auxiliary means such as computers can properly improve the accuracy of its measurement, these methods can only detect and measure low-dose radiation, and there is no method for biological detection and measurement of high-dose radiation