Method for culturing orchid special strain thereof

Abstract

The invention discloses a method for culturing an orchid and a special bacterium thereof. The name of the bacterium of the invention is GDB181 the preservation number of which is CGMCCNo.2574. The method of the invention includes the steps for together culturing the bacterium of the invention and the orchid. The bacterium of the invention is applicable for the root culturing of an orchid bacteriaand builds an epiphyte bacterium with excellent root which effectively coexists with the orchid. The method of the invention for culturing the orchid can better control the inoculation amount and canfix the inoculation position, thus fully ensuring the tissue culture seedling of the orchid to build the coexistence relation with the epiphyte bacterium with excellent root, simultaneously simplifying the inoculation steps and reducing the inoculation times. Permanent effect can be achieved by being inoculated for once, thus avoiding the dead seedling phenomenon caused by a plurality of manual inoculations, thereby improving the survival rate of transplanting and obtaining the root orchid seedling which grows strongly. Therefore, the method for culturing bacterium, the culture medium of the bacterium and the culture medium of the orchid of the invention has important economic values and is applicable for popularization and application.

Description

technical field [0001] The invention relates to a method for cultivating orchids and special strains thereof. Background technique [0002] Orchidaceae is one of the largest groups in the plant kingdom, widely distributed in temperate, subtropical and tropical regions, most of which are rare medicinal plants and famous ornamental flowers. Orchids are both ornamental and medicinal. They not only have a large number of fans, but also become an important part of Chinese traditional culture - orchid culture. [0003] Orchidaceae are typical endomycorrhizal plants, which absorb mineral nutrients and water from the surrounding environment through the symbiotic mycorrhizal fungus hyphae, and then quickly transport nutrients to the orchid root system through the protoplasmic circulation inside the mycelium. so as to provide for its growth. The orchid itself can also absorb nutrients by digesting the mycorrhizal fungus hyphae that infect the interior of its cells. Mycorrhizal fung...

AI Technical Summary

Problems solved by technology

However, the current problem is that the survival rate of the tissue culture seedlings after transplanting is low, the growth is slow, the flowering is slow, and the flowers are small or even do not bloom, which seriously affects the number of subsequent orchids surviving, thereby failing to meet the needs of the market

The root cause of the above problems is that the mycorrhizalization technology of orchid tissue culture seedlings is immature, as follows: 1. The lack of good mycorrhizal fungi symbiotic with orchids cannot form effective mycorrhizae, resulting in the lack of orchid nutrient absorption; 2. , lack of effective mycorrhizal fungus inoculum agent, after inoculation, the nutrient source of the fungus cannot be guaranteed, and multiple inoculations are required, which will greatly damage the root system of orchid seedlings; three, the timing of inoculation and the amount of inoculation are not easy to control, and it is easy to cause artificial Death of orchid seedlings caused by inoculation

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