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Saccharose determination reagent kit and method for determining saccharose concentration

A sucrose and reagent technology, applied in the field of food inspection and measurement, can solve the problems of poor accuracy and cumbersome sucrose content process

Inactive Publication Date: 2008-12-24
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the cumbersome process and poor accuracy of measuring sucrose content by conventional titration, there are also high performance liquid chromatography to detect samples.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The sucrose assay reagent of this embodiment is a single reagent, comprising:

[0039] Phosphate buffer 100mmol / L

[0040] Stabilizer 500mmol / L

[0041] Reduced coenzyme 0.25mmol / L

[0042] Sucrose phosphorylase 10000U / L

[0043] Mannitol dehydrogenase 12000U / L

[0044] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0045] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the sucrose sample to the reagent is 1 / 25, The reaction direction is negative reaction (decreasing reaction), the delay time is about 0 minutes, and the detection time is about 5 minutes.

[0046] After adding the samples and reagents, let them mix and react, and finally put the reactants under the biochemical analyze...

Embodiment 2

[0048] The sucrose determination reagent of the present embodiment is double reagent, comprises:

[0049] Reagent 1

[0050] Phosphate buffer 100mmol / L

[0051] Stabilizer 50mmol / L

[0052] Reduced coenzyme 0.25mmol / L

[0053] Reagent 2

[0054] Phosphate buffer 100mmol / L

[0055] Stabilizer 500mmol / L

[0056] Sucrose phosphorylase 10000U / L

[0057] Mannitol dehydrogenase 12000U / L

[0058] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0059] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test sub-wavelength 405nm, the volume ratio of the tested sucrose sample to reagent 1 and reagent 2 is 2 / 20 / 5, the reaction direction is negative reaction (downward reaction), the delay time is about 0 minutes, and the detection time is about 5 minutes.

[0060] After adding the samples and reag...

Embodiment 3

[0062] The sucrose determination reagent of the present embodiment is three reagents, comprises:

[0063] Reagent 1

[0064] Phosphate buffer 100mmol / L

[0065] Stabilizer 50mmol / L

[0066] Reduced coenzyme 0.25mmol / L

[0067] Reagent 2

[0068] Phosphate buffer 100mmol / L

[0069] Stabilizer 500mmol / L

[0070]Mannitol dehydrogenase 12000U / L

[0071] Reagent 3

[0072] Phosphate buffer 100mmol / L

[0073] Stabilizer 500mmol / L

[0074] Sucrose phosphorylase 10000U / L

[0075] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0076] When measuring the concentration of sucrose, set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, test sucrose sample and reagent 1, reagent 2. The volume ratio of reagent 3 is 4 / 40 / 5 / 5, the reaction direction is negative reaction ...

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Abstract

The invention relates to a saccharose determination kit which uses the techniques of an enzyme colorimetry method and an enzyme-linked method; meanwhile, the invention also relates to a method principle used for determining saccharose consistency, reagent composition and component, belonging to the field of food detection determination technique. The main components of the kit of the invention comprise phosphoric acid buffer solution, reduction-typed coenzyme, sucrose phosphorylase, mannitol dehydrogenase and stabilizer; samples and reagents are mixed according to a certain volume proportion so as to generate a series of enzymatic reactions; subsequently, reaction matters are arranged under an ultraviolet / visible light analyser so as to detect the degree / speed of the absorbency reduction of the main wavelength at the 340nm position, thus calculating the consistency of the saccharose.

Description

technical field [0001] The invention relates to a sucrose determination kit, and at the same time, the invention also relates to a method for determining sucrose concentration, which belongs to the technical field of food inspection and determination. Background technique [0002] At present, the phenomenon of honey adulteration is serious. According to the survey, in the honey purchased at the grassroots level, among the adulterated samples, the sucrose content is mostly between 10-28%, and some are even as high as 50%. In addition, it is common to add sugar in the processing of medium and low-grade tea to increase the weight and seek illegal benefits. It is often heard that sugar-free foods contain sucrose, which is harmful to diabetics. [0003] Application patent number 200510099310.4 is a method for detecting sucrose content in sugarcane juice, disclosing a method for determining sucrose, the method includes hydrolysis of sucrose, reaction of reducing sugar with potass...

Claims

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Application Information

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IPC IPC(8): G01N21/31C12Q1/26C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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