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Use of Carex rigescens in preparing antivirus medicament

A technology of Carex sylvestris and medicine, applied in the field of application of Carex sylvestris in preparing antiviral drugs

Inactive Publication Date: 2008-12-24
高川
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no data report on the antiviral activity of Carex spp. or Carex spp.

Method used

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  • Use of Carex rigescens in preparing antivirus medicament
  • Use of Carex rigescens in preparing antivirus medicament
  • Use of Carex rigescens in preparing antivirus medicament

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0041] Example: Study on the effect of Carex radix extract on viruses:

[0042] (1) Preparation of Carex alba extract:

[0043] Carex alba was extracted by physical methods (fresh grass juice), boiled, water / acetone extraction, water / ethanol extraction to obtain fresh juice ①, water extract ②, acetone extract ③ and alcohol extract ④, respectively, Extract ③ or ④ with petroleum ether, chloroform, and ethyl acetate, and take the extracted part of ethyl acetate as ⑤, separate ⑤ with a silica gel column, and use chloroform / methanol gradient elution to collect the chloroform / methanol 6: 4. The elution fraction of 5:5 and 4:6 is ⑥. The above extracts were respectively dissolved in cell maintenance solution (MEM containing 2% newborn calf serum, product of GIBICO Company) for later use.

[0044] (2) Cell strains and virus strains:

[0045] Human embryonic kidney cells (HEK293, sensitive to HSV 1, HSV 2, and RSV) were purchased from Clontech, USA; human embryonic lung cells P12 (HE...

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Abstract

The invention discloses an application of carex rigescens in preparing antivirus medicine. The testing shows that the carex rigescens has significant inhibitory effects to HSV-1, HSV-2, HCMV, RSV and flu A, which implicates that the carex rigescens has potential application value in remedying various virus infectious disease fields. The carex rigescens has especially important therapeutic significance for infectious diseases of herpesviridae (HSV-1, HSV-2, HCMV). The application provides an experimental basis for the carex rigescens clinically applying in remedying virus infectious diseases, provides a certain guide significance for developing medicines which can against HSV-1, HSV-2, HCMV, RSV AND Flu A virus, and has significant reference value.

Description

technical field [0001] The present invention relates to the anti-herpes simplex virus type 1 (Herpes simplex virus, HSV-1), herpes simplex virus type 2 (Herpes simplex virus, HSV-2), human cytomegalovirus (Human cytomegalovirus, HCMV), respiratory syndrome It is used in the medicine of Respiratory Syncytial Virus (RSV) and Influenza A virus (Flu-A). Background technique [0002] Carex sedge, Cyperaceae, Carex genus, Latin: Carex rigescens (Franch.) V.Krecz. (C.stenophyllaWahlb.var.rigescens Franch.), also known as small sheep beard grass, distributed in Liaoning, North my country in China , Shandong, Henan, Northwest and other places, common in grasslands, hillsides and riversides, is a perennial plant, morphological characteristics: with slender creeping rhizomes, culm height 5-40 cm, dark brown fibrous split old leaf sheaths at the base . Leaves shorter than culms, 1-3 mm wide, flattened. Spikes ovate or oblong, with 5-8 spikelets, densely grown at the end of the culm; sp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/89A61P31/22A61P31/16A61P31/12
Inventor 高川
Owner 高川
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