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Method for determining galactose concentration and galactose diagnosis/determination reagent kit

A technology of galactose and kits, which is applied in the field of medical/food inspection and determination, and can solve problems affecting infants' physical and intellectual development, element absorption disorders, etc.

Inactive Publication Date: 2008-12-17
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003]Although milk is good, not all people can enjoy it. Due to the lack of lactase, the human body cannot digest and absorb the nutrients in milk well, and will Causes absorption disorders of calcium, phosphorus, potassium, iron and other elements, affecting the physical and intellectual development of infants and young children. In the elderly, lactase deficiency is an important cause of osteoporosis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The galactose diagnosis / measurement reagent of this embodiment is a single reagent, including:

[0037] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0038] Stabilizer 500mmol / L

[0039] Reduced coenzyme 0.25mmol / L

[0040] Galactose oxidase 12000U / L

[0041] NADH peroxidase 16000U / L

[0042] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0043] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the measured galactose sample to the reagent is 1 / 25 , the reaction direction is negative reaction (decreasing reaction), the delay time is about 0 minutes, and the detection time is about 5 minutes.

[0044] After adding the sample and reagent, let them mix and react, and...

Embodiment 2

[0046] The galactose diagnosis / measurement reagent of this embodiment is a double reagent, comprising:

[0047] Reagent 1

[0048] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0049] Stabilizer 50mmol / L

[0050] Reduced coenzyme 0.25mmol / L

[0051] Reagent 2

[0052] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0053] Stabilizer 500mmol / L

[0054] Galactose oxidase 12000U / L

[0055] NADH peroxidase 16000U / L

[0056] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0057] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test sub-wavelength 405nm, the volume ratio of the measured galactose sample to reagent 1 and reagent 2 It is 2 / 20 / 5, the reaction direction is negative reaction (downward reaction), the delay time is about 0 minutes, and ...

Embodiment 3

[0060] The galactose diagnosis / measurement reagent of the present embodiment is three reagents, comprising:

[0061] Reagent 1

[0062] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0063] Stabilizer 50mmol / L

[0064] Reduced coenzyme 0.25mmol / L

[0065] Reagent 2

[0066] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0067] Stabilizer 500mmol / L

[0068] NADH peroxidase 16000U / L

[0069] Reagent 3

[0070] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0071] Stabilizer 500mmol / L

[0072] Galactose oxidase 12000U / L

[0073] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0074] When measuring the concentration of galactose, set it on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, tested gal...

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Abstract

The invention relates to a kit for diagnosing / mensurating galactose by utilizing the technologies of the enzymic colorimetric method and the enzyme linked immunosorbent assay (ELISA). The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the concentration of the galactose, and belongs to the technology field of medical / food inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, galactose oxidase, NADH peroxidase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet / visible light analyzer, and the degree / velocity of the decrease in absorbance at 340nm of the dominant wavelength is detected, thereby mensurating the concentration of the galactose.

Description

technical field [0001] The invention relates to a galactose diagnosis / measurement kit, and at the same time, the invention also relates to a method for measuring galactose concentration, which belongs to the technical field of medicine / food inspection and determination. Background technique [0002] Milk is known as "liquid gold" in the nutritional field. As early as 1992, seven departments in my country jointly proposed the slogan "a glass of milk strengthens a nation". Except for cellulose, milk contains almost all kinds of nutrients needed by the human body. After lactose in milk enters the human body, it is decomposed into glucose and galactose by the action of lactase in the small intestine. Galactose is an essential substance for the development of the baby's brain and is closely related to the rapid growth of the baby's brain. [0003] Although milk is good, not all people can enjoy it. Due to the lack of lactase, some people cannot digest and absorb the nutrients i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/66G01N21/31C12Q1/26C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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