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Method for synthesizing poly(hydroxyalkanoate)

A technology of polyhydroxyalkanoic acid and polysaccharide, which is applied in the field of synthesizing polyhydroxyalkanoic acid to achieve the effect of reducing operating cost and system stability

Active Publication Date: 2008-12-17
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there has been no report on the synthesis of PHA under anaerobic conditions using GAO-enriched activated sludge

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020]Enrichment culture of GAO: according to the culture method of the literature (Biotechnology and Bioengineering, 2001, 76(1): 17-31) and appropriate improvement, that is, the remaining sludge of the urban sewage treatment plant with phosphorus removal function is put into plexiglass In the prepared 3.5L reactor, add 2.75L of waste water with a phosphorus concentration of 1.3mg / L, an acetic acid concentration of 216.7mg-COD / L, and a pH of 6.8, and stir and react for 2 hours under anaerobic conditions, followed by aerobic aeration for 3 hours. After settling and draining, add 2.75L of fresh waste water, and carry out 3 cycles a day, 8 hours per cycle. After nearly three months of cultivation, at the end of each anaerobic cycle, the amount of phosphorus released by activated sludge microorganisms to absorb unit organic acid is less than 0.25mol phosphorus / C-mol acetic acid, and the amount of glycogen degradation to absorb unit organic acid is greater than 0.8 C-mol glycogen / ...

Embodiment 2

[0023] See Example 1 for the enrichment culture of GAO and the determination method of PHA in the sludge. The synthesis of PHA is as follows: change the pH of the influent to 7.2, and stir for 3 hours under anaerobic conditions, and the rest of the synthesis conditions are the same as in Example 1, and the content of PHA per gram of bacteria is 44 mg.

Embodiment 3

[0025] See Example 1 for the enrichment culture of GAO and the determination method of PHA in the sludge. The synthesis of PHA is as follows: change the concentration of propionic acid to 350mg-COD / L, and control the influent pH=7.6, stir under anaerobic conditions for 0.5h, and the rest of the synthesis conditions are the same as in Example 1, and the PHA content in each gram of bacteria is measured. 31mg.

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Abstract

The invention belongs to the environmental protection technology field, more particularly relating to a method that utilizes activated sludge rich in glycogen-accumulating organisms (GAO for short) to synthesize Polyhydroxyalkanoates (PHA for short) under anaerobic condition. Namely, the GAO is enriched by cultivating and acclimating the excess sludge of a wastewater treatment plant, short chain fatty acid is used as a carbon source in a sequencing batch reactor (SBR for short), and microbes in the activated sludge rich in the GAO are stirred for a period of time under anaerobic condition to synthesize the PHA by controlling influent PH and the concentration of ammonia nitrogen. The method provides a new method for synthesizing the PHA, and reduces the production cost of the PHA.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and in particular relates to a method for synthesizing polyhydroxyalkanoic acid (PHA) under anaerobic conditions by using activated sludge rich in polysaccharide bacteria (GAO). Background technique [0002] As we all know, with the development of economy and society, the "white pollution" caused by chemically synthesized plastics has become more and more serious. Currently, plastic waste is estimated at 2.5×10 7 The speed of t is accumulated in nature, and its landfill, incineration and other treatment processes have shown many disadvantages that affect the environment. Therefore, it is of great practical significance to strengthen the research and development of degradable plastics, strive for breakthroughs in technology and apply them in practice, and produce plastic products with biodegradability and reasonable price for environmental protection and sustainable development. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/62C12R1/01
Inventor 陈银广姜亚敏姚樱顾国维
Owner TONGJI UNIV
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