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Preparation of liquid beta-diastase

An amylase and preparation technology, which is applied in the field of enzyme preparation preparation, can solve the problems of high production cost, waste liquid treatment, and high product impurity content, and achieves the effects of low production cost, reduced production energy consumption, and low product impurity content.

Inactive Publication Date: 2011-06-29
无锡赛德生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the problems such as high production cost, high product impurity content and waste liquid treatment in the above-mentioned existing β-amylase preparation method, the applicant provides a kind of preparation method of liquid β-amylase preparation

Method used

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  • Preparation of liquid beta-diastase
  • Preparation of liquid beta-diastase
  • Preparation of liquid beta-diastase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Extraction:

[0028] (1) Preparation of extraction water: add preservatives ethyl p-hydroxybenzoate 0.025Kg, potassium sorbate 0.625Kg, sodium benzoate 0.875Kg, reducing agent sodium metabisulfite 1.25Kg, low sodium sulfite 3.75Kg in 250L water, stir to dissolve;

[0029] (2) Extraction: Take 50Kg bran and add it to the extraction water prepared in step (1); under normal pressure, the water bath is heated to 45°C to keep warm, adjust the pH to 4.5, stir once per hour, and extract for 6 hours;

[0030] 2. Solid-liquid separation: After leaching, solid-liquid separation is carried out with a plate frame or screw press;

[0031] 3. Fine filtration: The separated extract solution is quickly cooled to below 20°C, and 3.75Kg of diatomaceous earth is added to the extract solution, and fine filtration is carried out through a fine filter plate frame;

[0032] 4. Ultrafiltration concentration: the fine filtrate obtained in step 3 enters the ultrafiltration concentrator for u...

Embodiment 2

[0037] 1. Extraction:

[0038] (1) Preparation of extraction water: add preservative ethyl p-hydroxybenzoate 1.4Kg, potassium sorbate 14Kg, sodium benzoate 14Kg, reducing agent sodium metabisulfite 70Kg, low sodium sulfite 70Kg in 7000L water, stir and dissolve;

[0039] (2) Extraction: Weigh 1000Kg bran and add it to the extraction water prepared in step (1); under normal pressure, the water bath is heated to 55°C to keep warm, adjust the pH to 5.0, stir once per hour, and extract for 7 hours;

[0040] 2. Solid-liquid separation: After leaching, solid-liquid separation is carried out with a plate frame or screw press;

[0041] 3. Fine filtration: the separated extract solution is quickly cooled to below 20°C, and 84Kg of diatomaceous earth is added to the extract solution, and the fine filter plate frame is used for fine filtration;

[0042] 4. Ultrafiltration concentration: the fine filtrate obtained in step 3 is subjected to ultrafiltration concentration, and the temperatu...

Embodiment 3

[0047] 1. Extraction:

[0048] (1) Preparation of extraction water: add preservative ethyl p-hydroxybenzoate 9Kg, potassium sorbate 30Kg, sodium benzoate 150Kg, reducing agent sodium metabisulfite 450Kg, low sodium sulfite 150Kg in 30000L water, stir and dissolve;

[0049] (2) Extraction: Take 5000Kg bran and add it to the extraction water prepared in step (1); under normal pressure, the water bath is heated to 50°C to keep warm, adjust the pH to 6.5, stir once per hour, and extract for 8 hours;

[0050] 2. Solid-liquid separation: After leaching, solid-liquid separation is carried out with a plate frame or screw press;

[0051] 3. Fine filtration: The separated extract solution is quickly cooled to below 20°C, and 84Kg of diatomaceous earth is added to the extract solution, and fine filtration is carried out through a fine filter plate frame;

[0052] 4. Ultrafiltration concentration: the fine filtrate obtained in step 3 is subjected to ultrafiltration concentration, and the...

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Abstract

The invention relates to a preparation method of a liquid Beta-diastase, which is characterized by taking wheat bran as raw material. The preparation method comprises the following technological steps: the extraction of the wheat bran, solid-liquid separation, the fine filtration of an extract, ultra-filtration concentration, prescription, secondary fine filtration and the adjustment of enzyme activity. The preparation method adopts a preservative with proper matching and proper temperature and Ph so as to effectively inhibit bacterial growth and avoid putrefaction and enzyme deactivation; the obtained products have low content of impurity, contains low concentrate of industrial salt compared with that of a solid enzyme preparation and meets the requirements of General Standard for Food Additives easily. The preparation method of the invention is a brand new preparation technology of the liquid Beta-diastase and has the advantages of simple technology, high production efficiency, low production cost and being suitable for industrialized production.

Description

technical field [0001] The invention relates to a preparation method of an enzyme preparation, in particular to a preparation method of using plants to prepare a liquid beta-amylase preparation. Background technique [0002] β-amylase (also known as α-1,4-D glucan maltose hydrolase, E.C.3.2.1.2) is an exo-amylase, which can start from the non-reducing end of starch and catalyze the hydrolysis in sequence α-1,4-D glucosidic bond to generate maltose. At the same time, Walden inversion occurs, and the product changes from α-type to β-type, so it is called β-amylase. [0003] Since β-amylase cannot hydrolyze the α-1,6 bond of amylopectin, nor can it cross the branch point α-1,6 bond to cut the internal α-1,4 bond and 2 near the α-1,6 bond. ~3 α-1,4 bonds, so the β-limit dextrin with 2~3 glucose residues often hangs outside the side branch remains in the hydrolyzate. Even when β-amylase acts on amylose, it can only hydrolyze 70% to 90% of the starch into maltose, and the rest ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/26
Inventor 胡洪清华家荣
Owner 无锡赛德生物工程有限公司
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