Uses of smoked plum extract against virus, bacteria, mycoplasma or chlamydia of livestock and poultry
An extract and ebony technology, applied in the field of biomedicine, can solve the problems of lack of vaccines, the prevention and treatment of bacterial infectious diseases, etc., and achieve the effects of ensuring food safety, solving food antibiotic residues, and improving competitiveness.
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Embodiment 1
[0023] Embodiment 1 The preparation of ebony extract or effective part
[0024] Crush 1kg of ebony plums, then pass through a 10-mesh sieve, add 4-5kg of water to soak the sieve, decoct 3 times, 2-6 hours each time, collect the filtrate 3 times, concentrate to 1-1.5kg, and then use acetic acid Soak in 3-6 times the amount of ethyl ester, soak for 5-10 times continuously, collect the filtrate, recover ethyl acetate, and obtain the ebony extract;
[0025] After dissolving the ebony extract in methanol, mix it with silica gel 1-1.5 times (weight / weight), let it dry naturally, put it on a silica gel column, and use 100% chloroform, 90% chloroform (chloroform / methanol=9:1), 80% chloroform (chloroform / methanol=8:2), chloroform / methanol (2:1), chloroform / methanol (1:1), chloroform / methanol (1:1) / 1% formic acid, chloroform / methanol (1:1) 2) / 1% formic acid; collect 100% chloroform eluted fraction to obtain active ingredient I; collect 90% chloroform eluted fraction to obtain active in...
Embodiment 2
[0030] The preparation of embodiment 2 ebony extract granules and oral liquid
[0031] 100g of ebony extract was dissolved with 500ml of 95% ethanol, and 20g of sodium polyacrylate, 20g of sodium carboxymethylcellulose and 1200g of dextrin were added. Add medicinal starch 660g again, make granule 2000g according to conventional method.
Embodiment 3
[0032] The preparation of embodiment 3 ebony extract oral liquid
[0033] Dissolve 100g of ebony extract in 500ml of 95% ethanol, filter with a 100-mesh filter cloth, add 100g of sodium carboxymethylcellulose, mix well, dilute to 2000ML with water and pack into 100ML / bottle of oral liquid.
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