Process for producing 2,3-butanediol using american artichoke as raw material by fermentation
A technology of butanediol and Jerusalem artichoke, which is applied in the field of microbial fermentation production and fermentation production, can solve the problems of restricting large-scale production and wide application and influence, and achieve the effects of saving fermentation time, wide planting range and low price
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Embodiment 1
[0020] Using Jerusalem artichoke juice as raw material, adopt step-by-step saccharification and fermentation process:
[0021] Wash the Jerusalem artichoke tubers, press them, concentrate and adjust the paste to obtain the Jerusalem artichoke juice, add 30U / g of Jerusalem artichoke dry weight inulinase to the Jerusalem artichoke thick juice at 55°C and pH 6.0, and hydrolyze it for 12 hours to obtain a reducing sugar concentration of 254.2 g / L inulin hydrolyzate; sterilize 1L inulin hydrolyzate separately at 115°C for 15min, and mix it with 500mL sterilized fermentation medium after cooling; inoculate the seeds cultivated for 22 hours with 5% The fermentation culture was carried out under the conditions of 37°C, 300rpm, pH6.0, and aeration rate of 0.1vvm; the culture method adopts batch fed-batch fermentation, and when the concentration of residual reducing sugar is lower than 50g / L, additional 200ml of inulin hydrolyzate with a reducing sugar concentration of 368g / L was fermen...
Embodiment 2
[0023] Using Jerusalem artichoke powder as raw material, adopt step-by-step saccharification and fermentation process:
[0024] For the convenience of storage, wash, dry and crush Jerusalem artichoke tubers to make Jerusalem artichoke powder, add water to make a slurry before use; add 400g of Jerusalem artichoke powder to water to make a slurry and set the volume to 1L, and add 30U / g Jerusalem artichoke dry weight inulinase, hydrolyzed for 12 hours to obtain an inulin hydrolyzate with a reducing sugar concentration of 246.50g / L; sterilize it separately at 115°C for 15min, cool it with 500mL sterilized fermentation culture The inorganic salt components in the base were mixed; the seeds cultivated for 22 hours were inoculated according to the inoculation amount of 5%, and the fermentation culture was carried out at 37°C, 300rpm, pH6.0, and the ventilation rate was 0.1vvm; the cultivation method was batch fermentation. method, the fermentation was carried out for 56 hours, the f...
Embodiment 3
[0026] Using Jerusalem artichoke powder as raw material to adopt simultaneous saccharification and fermentation process:
[0027] Mix 400g of Jerusalem artichoke powder with water to make a slurry to 1L, sterilize it separately at 115°C for 15 minutes, and mix it with 500mL of sterilized fermentation medium after cooling; mix the seeds cultivated for 22 hours with 5% The amount of inoculum was added, and at the same time, the glucoamylase that had been sterilized by the membrane was added. The amount of glucoamylase added was 30U / g dry weight of Jerusalem artichoke; the fermentation culture was carried out at 37°C, 300rpm, pH6.0, and the ventilation rate was 0.1vvm The culture method adopts the mode of batch fermentation; when fermenting for 24 hours, the concentration of 2,3-BD in the fermentation broth is 48.95g / L, and the sum of the concentration of 2,3-BD and acetoin has reached 53.91g / L, the concentration of 2,3-BD was 70.11g / L when fermented for 40h, the sum of the conce...
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