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Application of plumbagin in preparing the medicine for preventing the blood vessel from regenerating

A technique for angiogenesis and plumbagin is applied in the application field of plumbagin in the preparation of angiogenesis-inhibiting drugs, and can solve the problems of no plumbagin inhibiting angiogenesis and the like.

Inactive Publication Date: 2008-10-15
EAST CHINA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous experimental results have shown that plumbagin has an anti-tumor effect and can treat acute promyelocytic leukemia, but there is no report about plumbagin inhibiting angiogenesis and acting as an angiogenesis-inhibiting drug

Method used

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  • Application of plumbagin in preparing the medicine for preventing the blood vessel from regenerating
  • Application of plumbagin in preparing the medicine for preventing the blood vessel from regenerating
  • Application of plumbagin in preparing the medicine for preventing the blood vessel from regenerating

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: plumbagin inhibits proliferation of endothelial cells.

[0021] Purpose and principle: MTS assay was used in endothelial cell proliferation assay. MTS assay is a colorimetric method to determine the number of cell proliferation. In living cells, NADPH dehydrogenase converts MTS tetrazolium salt compound into a colored substance soluble in tissue culture fluid. The number of living cells of these cell lines was highly correlated within a certain range. This model can be used to evaluate the effect of drugs on the proliferation ability of each batch of cells.

[0022] Method: Divide 1×10 5 cells / ml HMEC-1 (human microvascular endothelial cells) or HUVEC (human umbilical vein endothelial cells) cell suspension was inoculated in 96-well plates, 50 μl per well, and 50 μl of culture medium containing plumbagin with different concentrations were given at 5% CO 2 Incubate at 37°C for 48 to 72 hours, then add 20 μl of detection solution to each well, incubate for ...

Embodiment 2

[0024] Example 2: plumbagin inhibits endothelial cell migration

[0025] Purpose and principle: The cell migration test is also called in vitro scratch test. In this experiment, scratches were made on a single layer of endothelial cells, and the migration of cells at the edge of the scratches to the gap was observed and recorded under a microscope to evaluate the effect of drugs on the migration ability of endothelial cells.

[0026] Method: Place 4×10 4 Each / ml HUVEC cells were seeded in a 6-well plate, 2ml / well. Make a scratch in each well after the cells grow to more than 90%. Wash the dead cells floating on the upper layer at this time, and then add the medium containing 4ng / ml VEGF with different drug concentrations into the six-well plate, in 5% CO 2 Culture in an incubator at 37°C.

[0027] Results and evaluation: Observing under a microscope, after the scratch gaps in the VEGF control group were completely covered by cells, photographs were taken under the microsco...

Embodiment 3

[0028] Example 3: Experiment of plumbagin inhibiting endothelial cell migration in Boyden chamber

[0029] Purpose and principle: Boyden cell culture chamber was used in this experiment. Under the induction of growth factors, cells can pass through the polycarbonate filter membrane with a pore size of 8 μm. This experiment can detect the effect of drugs on the migration ability of endothelial cells under resistance conditions.

[0030] Method: Coat the 6-well plate with 0.1% gelatin, add 600 μl of VEGF containing 10ng / ml to the six-well plate outside the small chamber 165 endothelial cell culture medium. The concentration of 50 μl inserted into the small chamber is 4×10 5 cells / ml HUVEC cell suspension and 50 μl medium containing different concentrations of plumbagin, 37°C 5% CO 2 Incubate for four hours in the incubator.

[0031] Results and evaluation: Four hours later, the cell culture medium inside and outside the small chamber was aspirated, and the migrating cells in...

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Abstract

The invention relates to a new application of plumbagin, particularly to an application of the plumbagin in preparing drugs for inhibiting tumor angiogenesis, psoriasis, Kaposi's sarcoma, diabetic retinopathy, aleukemic leukemia, rheumatoid arthritis, duodenal ulcer, myeloma, and lymphomata. The experimental results show that the plumbagin can inhibit the proliferation, migration and canalization of endothelial cells, and inhibit the angiogenesis of chick embryo allantoic membrane. Therefore,the plumbagin can be used for preparing drugs for inhibiting the angiogenesis.

Description

technical field [0001] The invention relates to a new application of plumbagin, in particular to the application of plumbagin in the preparation of drugs for inhibiting angiogenesis. Background technique [0002] Angiogenesis is the process in which microvessels form new vascular branches and capillary clusters from existing blood vessels, and is the result of proliferation, migration and remodeling of mature endothelial cells in blood vessels. Angiogenesis includes five stages: ① vascular cells secrete proteolytic enzymes to degrade the vascular basement membrane; ② endothelial cells migrate through the basement membrane to the perivascular matrix; ③ endothelial cells proliferate, adhere to each other and connect; ④ form a lumen-like structure; ⑤ The matrix remodeling, the enveloping of smooth muscle cells and the mutual anastomosis of blood vessels form a vascular network. [0003] Angiogenesis occurs under both physiological and pathological conditions. Physiological an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/122A61P1/04A61P9/10A61P15/00A61P15/08A61P17/06A61P19/02A61P25/02A61P27/02A61P27/06A61P29/00A61P35/00A61P35/02
Inventor 易正芳赖力张京张晓丽卢彬彬易庭芳刘明耀
Owner EAST CHINA NORMAL UNIV
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