Hydrophobic electric charge induced type thiophil expanded adsorbent bed medium and method for producing the same

Abstract

The invention discloses a charge-induced sulphophile expanded adsorbent bed medium with hydrophobic nature and a preparation method thereof. In the composition of the medium, matrix is a composite microsphere with fibrin / inorganic weighting agent, petunidin is sulfonyl group and mercapto benzimidazole. The composite microsphere with fibrin / inorganic weighting agent is acquired by inverse suspension thermal regeneration and is taken as the matrix to mix divinyl sulfone, soda buffer solution and DMSO for activation, then swab-off active matrix, the mercapto benzimidazole and sodium hydroxide containing ammonium persulfate are mixed for coupling, thus acquiring the charge-induced sulphophile expanded adsorbent bed medium with the hydrophobic nature. The expanded bed adsorbing medium developed by the invention combines with three protein separation principles of hydrophobic charge induced chromatography, thiophilic chromatography and expanded bed adsorption, etc., which has the advantagesof large density of the petunidin, large protein adsorption capacity, strong specificity, mild conditions of adsorption and elution, etc., and shows the characteristic of the integration of technology and process, thus being used for high efficient separation and purification of proteins such as antibody, etc.

Description

technical field The invention relates to a hydrophobic charge-induced thiophilic expanded bed adsorption medium and a preparation method thereof, belonging to the expanded bed adsorption and separation protein technology in chromatographic separation. Background technique With the rapid development of genetic engineering, protein engineering, fermentation engineering, and cell engineering, more and more biological products require efficient biological downstream technologies for separation and purification, and new requirements have been put forward for biological separation methods. Antibodies are an important biotechnology product widely used in immunotherapy, disease prevention, and medical diagnosis. The technology of preparing polyclonal antibodies and monoclonal antibodies through cell engineering and immune methods has become more and more mature, the types of antibodies are increasing, and the scale of preparation is also expanding, which requires efficient antibody...

AI Technical Summary

Problems solved by technology

However, none of these HCIC media clearly binds to thiophile, and is a medium with a density close to 1. It can only be used in conventional packed chromatography, and cannot be used in the newly emerging expanded bed adsorption process.

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