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Method for producing active yeast cell derivative

A production method and technology of derivatives, applied in the field of production of live yeast cell derivatives, can solve the problems of high cost, moisturizing, anti-aging effect to be improved, and great difficulty, and achieve low production cost, shortened production cycle, and convenient operation Effect

Inactive Publication Date: 2008-08-27
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing preparation methods of active yeast derivatives all have the disadvantages of difficulty and high cost, and their moisturizing and anti-aging effects still need to be improved

Method used

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  • Method for producing active yeast cell derivative

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Effect test

Embodiment 1

[0024] A method for producing live yeast cell derivatives (LYCD), comprising the steps of:

[0025] (1). Strain selection: use microbial live yeast as the strain;

[0026] (2). Preparation of yeast culture medium: using wort juice 10 0 Brix is ​​used as the slant medium, and YEPD medium is used as the liquid medium. The yeast stored in the wort slant medium is transferred to the liquid medium, and cultured at 28°C until the logarithmic phase for 12 hours to prepare the yeast culture solution ;

[0027] (3).Extreme high temperature stimulation: the adaptive temperature of the yeast culture medium was changed to a high temperature of 35°C for 30 minutes, and the yeast culture medium was at a high temperature of 35°C in the early, middle and late logarithmic stages;

[0028] (4). Cell collection: use a refrigerated centrifuge to centrifuge the yeast culture solution after high temperature stress at 4000 rpm for 10 minutes, and collect yeast cells;

[0029] (5). Thalline cell w...

Embodiment 2

[0048] A method for producing live yeast cell derivatives (LYCD), comprising the steps of:

[0049] (1). Strain selection: use microbial live yeast as the strain;

[0050] (2). Preparation of yeast culture medium: using wort juice 12 0 Brix is ​​used as the slant medium, and YEPD medium is used as the liquid medium. The yeast stored in the wort slant medium is transferred to the liquid medium, and cultured at 30°C until the logarithmic phase for 8 hours to prepare the yeast culture solution ;

[0051] (3).Extreme high temperature stimulation: the adaptive temperature of the yeast culture medium was changed to a high temperature of 37°C for 28 minutes, and the yeast culture medium was at a high temperature of 37°C in the early, middle and late logarithmic stages;

[0052](4). Cell collection: use a refrigerated centrifuge to centrifuge the yeast culture solution after high temperature stress at 4000 rpm for 8 minutes, and collect the yeast cells;

[0053] (5). Thalline cell ...

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Abstract

The utility model relates to a method for producing a live yeast cell derivative, which comprises following steps: selecting yeast as a bacteria source, adopting wort as inclined medium, adopting YEPD medium which is suitable for the yeast to grow as liquid medium to culture liquid spawn, culturing the liquid spawn to a logarithmic phase 8 hours-12 hours, obtaining yeast culture medium, transforming the yeast culture medium from the most suitable temperature to the high temperature of 37 DEG C, stressing for 30 minutes, adopting a refrigerated centrifuge to separate the yeast culture medium centrifugally, collecting yeast thalli, adopting a high pressure homogenizer to crush yeast thalli cell wall, centrifugally separating the yeast cells for the second time, removing sediment, and obtaining the live yeast cell derivative. The method does not adopt a traditional protease hydrolysis method to crush the wall but selects the high pressure homogenizer to crush the yeast cells, the crushing effect is good, the operation is convenient, the method is easily extended and applied and can realize industrialization, and simultaneously the method has the advantages of low production cost, little investment and strong practicability.

Description

technical field [0001] The invention belongs to the field of bioactive additives, in particular to a production method of live yeast cell derivatives. Background technique [0002] With the development of the economy and the continuous improvement of people's living standards, people have higher requirements for health, and they are increasingly aware that there are many factors that are unfavorable to health in the living environment and working environment. In order to effectively prevent the damage to the body caused by the adverse external environment and enhance the body's resistance, it is necessary to use functional cosmetics, food and pharmaceutical products with moisturizing and anti-aging properties, and these products are more and more popular among people. [0003] At present, as functional cosmetics, food and pharmaceutical products, there are three main methods for extracting bioactive additives: 1. From animal glands (such as thymus, spleen, placenta, brain an...

Claims

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Application Information

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IPC IPC(8): C12N1/16A61K8/99A61K36/064A23L1/28A23L1/30A23L31/10A23L33/14
Inventor 路福平高艳玲黄正梅王春霞李玉
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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