Tuberculosis and medicament-resistant tubercular personalized yelk polyclone antibody and method of preparing the same and applications

A polyclonal antibody, tuberculosis technology, applied in the direction of antibodies, antibacterial drugs, antibacterial immunoglobulins, etc., can solve the problems of difficult to cure, long course of treatment, increased toxicity and side effects, and achieve high accuracy of test results and large applications. Foreground, easy-to-use effects

Inactive Publication Date: 2008-08-27
沈星灿
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that there are four major shortcomings in the treatment of tuberculosis with Western medicine: ① The course of treatment is long, the initial treatment should be at least 9 months, and the retreatment should be at least 1.5 years
②The cost of treatment is expensive and difficult to cure
③ There are relatively large toxic and side effects, and the treatment of this disease requires combination medication, and the toxic and side effects are also increased
④ Further exacerbating the emergence of multi-drug resistant bacteria
Egg yolk antibody has been used in veterinary clinics, but so far, there is still no egg yolk antibody product used in the clinical treatment and detection of human tuberculosis and drug-resistant tuberculosis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A preparation method for sensitive tuberculosis-specific polyclonal IgY antibody preparation comprises the steps:

[0032] (1) Personalized real-time immunization:

[0033] Take the serum or sputum of a patient infected with sensitive Mycobacterium tuberculosis, cultivate sensitive Mycobacterium tuberculosis, inactivate with 0.4% formaldehyde, and make a personalized vaccine for patients infected by sensitive Mycobacterium tuberculosis; , specific pathogen-free) hens, adaptive feeding, one week after normal laying, began to immunize. Inject personalized vaccine antigen (susceptible tuberculosis) in the amount of 10-1000 μg, add Fred's adjuvant, 0.5 mL each time, subcutaneously or intramuscularly, immunize once every 7 days, a total of 4 times, the antibody is usually in the first immunization Appeared after 14 days. Boost immunization once every 30 days, collect eggs, and store at 4°C;

[0034] (2) Antibody purification and preparation preparation:

[0035] Disinfect ...

Embodiment 2

[0042] The preparation of a kind of rifampicin-resistant tuberculosis specific polyclonal IgY antibody preparation comprises the steps:

[0043] (1) Personalized real-time immunization:

[0044] Take the serum or sputum of a patient infected with rifampicin-resistant Mycobacterium tuberculosis, cultivate rifampicin-resistant Mycobacterium tuberculosis, inactivate with 0.4% formaldehyde, and make a personalized vaccine against Rifampicin-resistant Mycobacterium tuberculosis.

[0045] Select SPF hens, feed them adaptively, and start immunization after one week of normal laying. The amount of antigen (rifampicin-resistant Mycobacterium tuberculosis) in the injection of personalized vaccine is 10-1000μg, plus Freud's adjuvant, 0.5mL each time, subcutaneous or intramuscular injection, immunization once every 7 days, a total of 2-4 times, antibody It usually appears 14 days after the first immunization. Immunization can be boosted once every 30 days. Collect eggs and store at 4°C...

Embodiment 3

[0052] Preparation and application of a polyclonal IgY antibody preparation specific to isoniazid and rifampicin-resistant multidrug-resistant tuberculosis:

[0053] Except that the individualized vaccine adopts isoniazid and rifampicin multidrug-resistant Mycobacterium tuberculosis, the other processes are the same as in Example 1 or Example 2.

[0054] The applicable objects are patients infected with isoniazid and rifampicin-resistant multidrug-resistant Mycobacterium tuberculosis and high-risk infection groups, and the specific application method is the same as that in Example 1.

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PUM

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Abstract

The invention discloses a yolk polycolonal antibody formulation of personalized tuberculosis and drug-resistance tuberculosis, a preparation method, and application thereof. Pathogenic mycobacterium tuberculosis or dru-resistance tuberculosis is cultivated after being separated from the serum or sputum of tuberculosis patients, and then produced into personalized vaccine through the inactivating process of formaldehyde which can be used for the immune injection of healthy hen birds; specific polycolonal IgY antibody aimed at the patients and the pathogenic mycobacterium tuberculosis can be obtained from the yolk produced by immunized hen birds; after degermation, extraction, purification, liposome microencapsulation, etc., the specific polycolonal IgY antibody formulation can be obtained, which can be used for the passive immunotherapy of the patients with tuberculosis or drug-resistance tuberculosis and for preventing tuberculosis for high-risk population. The formulation is a safe, high-effect, and no-drug-resistance personalized biological formulation, which can also be applied to all the detecting methods on the basis of tuberculosis antigen-antibody immunoreaction, and can quickly and individually detect the infection of tuberculosis or drug-resistance mycobacterium tuberculosis.

Description

Technical field: [0001] The invention relates to the development of new biological drugs for treating and preventing tuberculosis and drug-resistant tuberculosis and new reagents for detecting tuberculosis and drug-resistant tuberculosis, especially the personalized yolk polyclonal antibody preparation for tuberculosis and drug-resistant tuberculosis and its preparation method and application. Background technique: [0002] Tuberculosis is one of the infectious diseases that seriously threaten global public health. According to WHO estimates, there are currently about 2 billion people infected with Mycobacterium tuberculosis worldwide, of which about 50 million people are infected with drug-resistant Mycobacterium tuberculosis. From 1994 to 1997, WHO / IUATLD (International Union Against Tuberculosis and Lung Disease) made statistics in 35 countries and regions: the initial drug resistance rate was 9.9%, the acquired drug resistance rate was 5.3% to 100%, and the multidrug res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/40C07K16/02C07K16/12G01N33/569A61P31/06
Inventor 沈星灿徐庆戴志刚梁宏谭宁陈秋娟唐振祥梁荣感李利亚
Owner 沈星灿
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