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Breast cancer biological molecule marker LASS2 protein, modifying product and medical composition thereof

A technology of biomolecules and breast cancer, applied in the field of protein, can solve the problems of low detection sensitivity and specificity, failure to meet clinical needs, cumbersome methods, etc., to improve diagnostic accuracy and resolution, increase discovery rate, reduce side effects

Inactive Publication Date: 2008-08-13
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these breast cancer biomolecular markers have been used clinically, the detection sensitivity and specificity are not strong, and some markers are only applicable to certain subtypes
In Chinese Patent Application No. 200480033043.4, a molecular marker is disclosed, which can be used to predict the diagnosis of cancer, especially the risk of breast cancer, but the method of using this molecular marker is cumbersome and has poor sensitivity
[0004] In conclusion, although some breast cancer biomolecular markers have been used clinically, these indicators are far from meeting the clinical needs, so it is urgent to develop new biomolecular markers for early diagnosis and prognosis analysis of breast cancer

Method used

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  • Breast cancer biological molecule marker LASS2 protein, modifying product and medical composition thereof
  • Breast cancer biological molecule marker LASS2 protein, modifying product and medical composition thereof
  • Breast cancer biological molecule marker LASS2 protein, modifying product and medical composition thereof

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Experimental program
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Effect test

Embodiment 1

[0036] The invention provides a method for detecting the expression of LASS2 protein in tissues by immunohistochemical method, and analyzes the expression difference of LASS2 protein in normal breast tissue, paracancerous tissue and different breast cancer tissues.

[0037] In order to determine the expression of LASS2 protein in normal breast tissue and breast cancer tissue, the expression of LASS2 protein was analyzed by immunohistochemical technique.

[0038] A total of 24 pairs of diagnosed breast cancer tissues and adjacent and normal tissues were selected. Among them, the cancer tissue is the tumor site, the paracancerous tissue is the tissue 1.5cm away from the tumor site, and the normal tissue is the distal tissue next to the cancer site. These tissue types have been identified by the pathology department of the hospital. Using the LASS2 protein mouse anti-human monoclonal antibody, the expression level of LASS2 protein was detected by immunohistochemical method.

Embodiment approach

[0040] 1. Antibody dilution concentration: 1:500;

[0041] 2. The negative control was replaced by primary antibody dilution;

[0042] 3. Experiment detailed staining steps:

[0043] 1) Bake the slices at 60°C for 30 minutes, dewax and hydrate them conventionally, and wash twice with deionized water;

[0044] 2) Retrieve the antigen with 0.01M citrate buffer (CB, pH6.0) under high temperature and high pressure, cool to room temperature, wash with phosphate buffer saline (PBS) for 5 minutes, repeat twice;

[0045] 3) Add normal non-immune animal serum blocking solution dropwise, room temperature for 15 minutes, and shake off excess liquid;

[0046] 4) Add the primary antibody dropwise, overnight at 4°C;

[0047] 5) Wash with 0.1% Tween phosphate buffered saline (Tween-PBS) for 5 minutes, repeat 3 times;

[0048] 6) Add biotin-labeled secondary antibody working solution dropwise, and incubate at 37°C for 15 minutes;

[0049] 7) Wash with 0.1% Tween phosphate buffered saline...

Embodiment 2

[0066] Provide Western Blot method to detect the expression of LASS2 protein in tissues and body fluids;

[0067] For clinical samples such as tissue, blood, and urine obtained during surgery, total protein was extracted according to routine procedures. The extracted total protein was subjected to polyacrylamide gel (SDS-PAGE) electrophoresis according to conventional procedures, and then transferred to PVDF membrane. The PVDF membrane was hybridized with LASS2 protein monoclonal antibody according to the conventional steps, and the expression of LASS2 protein in the total protein was detected by developing. LASS2 protein exists in breast cancer tissues and body fluids, confirming the above immunohistochemical findings.

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Abstract

The invention relates to a protein, specifically relates to a breast cancer molecular biomarker LASS2 protein, modified product and pharmaceutical composition thereof. The inventive project is: a breast cancer molecular biomarker LASS2 protein, wherein aminoacid sequence of LASS2 is shown in SEQ ID NO: 1; application of the breast cancer molecular biomarker in preparation of diagnosis reagent and therapeutic and preventive breast cancer medication. Advantages of the invention are: the breast cancer molecular biomarker LASS2 protein has a specific high expression in breast cancer tissue of different types and differentiations, and the invention has a vital importance in establishing novel diagnosis method for breast cancer, and establishing novel treatment method using receptor guided breast cancer target medicament.

Description

technical field [0001] The invention relates to a protein, in particular to a breast cancer biomolecular marker LASS2 protein, its modified product and a pharmaceutical composition. Background technique [0002] Breast cancer is the most common malignant tumor in women worldwide. In the past 20 years, the incidence of breast cancer in the world has almost doubled. In recent years, the incidence of breast cancer in China, especially in developed regions, has shown a rapid growth trend, and has leapt to the top of the incidence of female malignant tumors, seriously endangering the health of women. In the treatment of breast cancer, screening high-risk patients for corresponding radiotherapy and chemotherapy will make the treatment more targeted. At present, the diagnosis of breast cancer is mainly based on physical examination, mammography, nuclear magnetic resonance, B-ultrasound and pathological diagnosis. These physical examination methods can detect tumors from different...

Claims

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Application Information

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IPC IPC(8): C07K14/47C07K1/107A61K38/17A61K39/395A61K48/00A61K49/00G01N33/577A61P35/00
Inventor 郭妍林标扬徐学敏
Owner SHANGHAI JIAO TONG UNIV
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