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Method of detecting large bowel cancer marker

A detection method and technology for colorectal cancer, applied in biochemical equipment and methods, microbiological measurement/testing, recombinant DNA technology, etc., can solve the problems of insufficient sensitivity of stool occult blood method

Inactive Publication Date: 2008-07-30
HAMAMATSU FOUNDATION FOR SCIENCE & TECHNOLOGY PR
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Problems solved by technology

[0015] Recently, although a method for detecting the expression of CD44 mutants in fecal cell fractions was developed to replace the fecal occult blood method as a colorectal cancer screening method, the sensitivity of this method is only about 68%, which is not as good as that of the fecal occult blood method. Sensitivity (about 75%) (Non-Patent Document 8)

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  • Method of detecting large bowel cancer marker

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Embodiment 1

[0066] While the invention is illustrated by the following examples, the invention is not limited thereto.

[0067] Patients admitted for thorough examination and treatment at the First Department of Internal Medicine of Hamamatsu Medical University, patients confirmed to have colorectal cancer by total colonoscopy, and patients without tumors or inflammatory changes in the large intestine (non-colorectal disease patients) were taken as object. In addition, informed consent was obtained from all patients.

[0068] Divide the feces into 5ml test tubes as soon as possible after collecting them, about 0.5-1g each, freeze them with liquid nitrogen, and store them at -80°C. In addition, as a control, human hemoglobin (Hb) in stool was measured for each sample by the immunological fecal occult blood test method. The biopsy materials of the cancerous part and the normal part at the endoscopic examination before the treatment were frozen with liquid nitrogen and stored at -80°C. Su...

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Abstract

A tumor marker detection method, which is a tumor marker detection method for diagnosing colorectal cancer and colorectal adenoma comprising the following steps: a) collecting feces and freezing it; The step of homogenizing the frozen feces in the presence of the suspension to prepare the suspension; c) the step of extracting RNA from the obtained sample for RNA extraction; d) the step of reverse-transcribing the extracted RNA to obtain cDNA; e) a step of amplifying the obtained cDNA, and f) a step of detecting the amplified cDNA, characterized in that the tumor marker is at least one tumor marker selected from COX-2, SNAIL, and MMP-7 ( But exclude only any one of COX-2 or MMP-7).

Description

technical field [0001] The present invention relates to one or more tumor markers selected from COX-2, SNAIL and MMP-7 for diagnosing colorectal cancer and colorectal adenoma (wherein, only any of COX-2 or MMP-7 is excluded. 1) A method for detection, and also relates to a kit for diagnosing colorectal cancer and colorectal adenoma containing these tumor markers. Background technique [0002] The number of patients dying from colorectal cancer is increasing year by year. Among all patients who died of cancer, the number of deaths due to colorectal cancer ranks fourth among males and second among females (statistics of cancer deaths in 1999). In addition, in 2015, cancer patients are expected to rise to the first place in both men and women. Therefore, comprehensive colorectal cancer countermeasures including secondary prevention are expected, and cancer population screening is one of the most effective methods. [0003] For cancer mass screening, a simple and non-invasive ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/09
CPCC12Q1/6886C12Q2600/112C12Q2600/158
Inventor 金冈繁
Owner HAMAMATSU FOUNDATION FOR SCIENCE & TECHNOLOGY PR
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