Acanthopanax senticosus effective fraction extract, preparation and application thereof

A technology of effective parts and extracts, which is applied in the field of traditional Chinese medicine, can solve the problems of high production cost, large production pollution, and low content of active ingredients, and achieve the effects of low cost, less environmental pollution, and easy operation

Inactive Publication Date: 2008-07-09
HARBIN RENHUANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of them use syringin as the quality control standard, and there are some injection varieties that only have a single effective fraction of total flavonoids, and the existing technology is mostly based on organic solvent extraction, which not only has high production costs, but also has relatively high pollution in industrialized production. Large, and there are very few reports on extracts containing total glycosides in effective parts
In the prior art, there is also a method of extracting the active ingredients of Acanthopanax senticosus using a certain mass fraction of ethanol as the extraction solvent, but the prepared active ingredient extract also has defects such as low content of active ingredients.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Get 30g Acanthopanax aqueous extract (conform to 2005 edition Chinese Pharmacopoeia), add the water dissolving of 30ml, through 50% ethanol precipitation, leave standstill, filter, collect filtrate, precipitate is washed with 50% ethanol solution, washing liquid reaches In the filtrate, the filtrate is concentrated to recover ethanol to obtain a crude extraction aqueous solution; after fully stirring, filter, adjust the pH value to 2, and absorb it through the macroporous adsorption resin D-101 (produced by Cangzhou Baoen Chemical Co., Ltd.) to adjust the pH value of the water eluted at 3; then eluted with a 20% methanol solution with a pH value of 3, collected the eluate fractions of this part, concentrated into a paste, and obtained the extract of total glycosides and total flavonoids in the effective part, which was detected by a UV spectrophotometer The total glucoside content analyzed is 61.52%, the total flavonoid content is 23.8%, the total content of the total gl...

Embodiment 2

[0031] Get 30g Acanthopanax aqueous extract (conform to 2005 edition Chinese Pharmacopoeia), add the water dissolving of 45ml, through 70% methanol precipitation, leave standstill, filter, collect filtrate, precipitate is washed with 70% methanol solution, and washing liquid reaches In the filtrate, the filtrate is concentrated to recover methanol to obtain a crude extraction aqueous solution, which is fully stirred, filtered, adjusted to a pH value of 4, and adsorbed by macroporous adsorption resin AB-8 (produced by Nankai University Chemical Factory) to prepare a 10% ethanol solution Adjust the pH value to 9 for elution; then elute with a pH value of 6 and a concentration of 40% ethanol solution, collect this part of the eluate fraction, concentrate it into a paste, and obtain the extract of total glycosides and total flavonoids in the effective part. Through the analysis of ultraviolet spectrophotometer, the total glucoside content is 32.6%, the total flavonoid content is 34...

Embodiment 3

[0033]Get 30g Acanthopanax aqueous extract (conform to 2005 edition Chinese Pharmacopoeia), add the water dissolving of 90ml, through 90% acetone precipitation, leave standstill, filter, collect filtrate, precipitate is washed with 90% acetone solution, and washing liquid reaches In the filtrate, the filtrate was concentrated and recovered acetone to obtain a crude extraction aqueous solution, which was fully stirred, filtered, adjusted to a pH value of 7, and adsorbed by a macroporous adsorption resin HPD-100 (produced by Cangzhou Baoen Chemical Co., Ltd.) with a concentration of 20 % acetone solution and adjust the pH value to 4 for elution; then elute with 60% acetone solution with a pH value of 9, collect the eluate fractions of this part, concentrate into a paste, and obtain the total glycosides and total flavonoids of the effective part The extract of the total glycosides is 21.57%, the total flavonoids content is 60.5%, and the total content of the total flavonoids is 82...

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PUM

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Abstract

The present invention discloses an extract of acanthopanax effective part, a preparation method thereof and an application thereof. In the acanthopanax extract of the present invention, total glycoside takes 20 to 64wt percent of the gross extract; total flavone takes 20 to 65wt percent of the gross extract; total glycoside total flavone takes 50 to 90wt percent of the gross extract. The preparation method of the acanthopanax effective part of the present invention is simple and convenient and causes little environmental pollution; the extracting and separating carrier can be used repeatedly for a plurality of times; the present invention has low cost and is applied to mass industrialization production.

Description

technical field [0001] The present invention relates to an extract of the effective part of a single plant, in particular to the extract of the effective part of Acanthopanax and its preparation method. Diseases, improving human immunity, anti-tumor, anti-radiation, and reducing the damage of radiation or chemotherapy drugs to the body belong to the field of traditional Chinese medicine. Background technique [0002] Acanthopanax is the dry root and rhizome of Acanthopanax senticosus (Puper.er Maxim) Harms, a plant of Araliaceae. Acanthopanax is a typical medicinal plant in Northeast my country. It is pungent, slightly bitter, warm in nature and non-toxic. Mainly distributed in my country's Heilongjiang, Jilin, Liaoning, Hebei and other places. [0003] Acanthopanax is a plant medicine with high medicinal value and a wide range of applications. In recent years, Chinese medicine workers have discovered that Acanthopanax has the effects of nourishing qi and strengthening the...

Claims

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Application Information

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IPC IPC(8): A61K36/254A61P9/10A61P25/24A61P15/12A61P35/00A61P37/04A61P17/16
Inventor 崔玉海张颖李男刘欢欢
Owner HARBIN RENHUANG PHARMA
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