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Technique for drying microorganism medicine sensitive detecting plate

A drying process and detection plate technology, applied in the field of microorganisms, can solve problems such as hole jumping, reduce the degree of antibiotic bacteriostasis, and detachment, and achieve the effects of improving quality, reducing costs, and ensuring accuracy

Inactive Publication Date: 2008-07-02
上海星佰生物技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0018] Due to the sublimation phenomenon in the vacuum freeze-drying process, the antibiotic aqueous solution is directly changed from solid to gas, and the antibiotic powder will not be attached to the wall of each hole with the sublimation process, instead of staying at the bottom completely, and due to the sublimation process, the antibiotic powder It is directly precipitated, and the formed antibiotic particles will be very small. These small particles are not easy to attach to the walls of each hole, and will detach during the transportation process, which will reduce the concentration of antibiotics in some holes, thereby reducing the concentration of antibiotics in some holes. The antibacterial degree of antibiotics may cause the MIC test results to skip holes, and the accurate MIC value cannot be judged, which will affect the quality of the test strips
The vacuum freeze-drying method is also used in the above-mentioned production process of drug-sensitive slats, so this drying process affects the quality of drug-sensitive slats to a certain extent

Method used

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  • Technique for drying microorganism medicine sensitive detecting plate
  • Technique for drying microorganism medicine sensitive detecting plate
  • Technique for drying microorganism medicine sensitive detecting plate

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0072] The specific production process of the drug sensitivity detection plate involved in the present invention is as follows:

[0073] (1) Configure antibiotic diluent

[0074] Add rosemary antioxidants to distilled water to configure an aqueous solution as a dilution of antibiotics;

[0075] (2) Packing

[0076] Dispense the prepared diluent into 15ml per test tube for use;

[0077] (3) Configure stock solution

[0078] Configure the initial stock solution of various antibiotics according to the dilution requirements;

[0079] (4) Dispensing liquid material

[0080] According to the dilution requirements of various slat concentrations, the antibiotic stock solution in step (3) is added to the corresponding subpackaged step (2) test tube.

[0081] (5) Adding samples and packing slats

[0082] Use the dispensing system to add samples to the designated drug-sensitive strips at the corresponding positions of the 96 wells, adding 50ul to each well;

[0083] (6) Slat dryin...

example 2

[0091] The specific production process of the drug sensitivity detection plate involved in the present invention refers to example 1

[0092] Wherein the drying process conditions are:

[0093] ①Ambient temperature: 24°C;

[0094] ② Drying equipment: hair dryer;

[0095] ③Ambient humidity: 34%

[0096] ④ Drying time: 21 hours.

example 3

[0098] The specific production process of the drug sensitivity detection plate involved in the present invention refers to Example 1,

[0099] Wherein the drying process conditions are:

[0100] ①Ambient temperature: 25°C;

[0101] ② Drying equipment: air dryer;

[0102] ③Ambient humidity: 40%

[0103] ④ Drying time: 18 hours.

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Abstract

The invention provides a drying process of an antimicrobial susceptibility test plate. The drying process has simple equipment and easy operation. The inventive drying process has equal drying effect to the vacuum freeze drying process, but is predominant in the analysis of a MIC value as test result. Therefore, the inventive drying process can reduce the cost, increase the accuracy of susceptibility test, and has high value in the clinical application.

Description

Technical field: [0001] The invention relates to the technical field of microorganisms. Specifically, it relates to a drying process for a microbial drug susceptibility detection plate. Background technique: [0002] In recent years, the emergence of bacterial drug resistance and the infection caused by drug-resistant strains have become increasingly serious, and nosocomial infection has become a global prominent problem. At present, in order to meet the professional needs of clinical laboratories for microbial identification and drug sensitivity equipment, the market has put forward higher requirements for the functions of microbial system products. The development and operation are simple, the identification range is wide, the drug sensitivity MIC value is accurate and practical, and it can be quickly given. Microbial products with experimental results have become an urgent requirement. [0003] At present, there are many microbial testing products in China, but there ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): F26B9/10
Inventor 王顺林邹艳芳张杰朱骏娜
Owner 上海星佰生物技术有限公司
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