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Pharmaceutical composition comprising an extract of pseudolysimachion longifolium and the catalpol derivatives isolated therefrom having antiinflammatory, antiallergic and antiasthmatic activity

A technology for allergic diseases and inflammatory diseases, which is applied in the fields of application, drug combination, and pharmaceutical formulation, and can solve problems such as the lack of reported or disclosed inhibitory effects of catalpol derivatives

Active Publication Date: 2012-07-04
KOREA RES INST OF BIOSCI & BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] [0007] Therefore, in any of the documents cited above, there is no report or disclosure about the effect of the extract of P. longifolium and the catalpol derivatives isolated therefrom on inflammatory diseases, allergic diseases and Inhibitory Effects on Asthma, the disclosure of which is incorporated herein by reference

Method used

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  • Pharmaceutical composition comprising an extract of pseudolysimachion longifolium and the catalpol derivatives isolated therefrom having antiinflammatory, antiallergic and antiasthmatic activity
  • Pharmaceutical composition comprising an extract of pseudolysimachion longifolium and the catalpol derivatives isolated therefrom having antiinflammatory, antiallergic and antiasthmatic activity
  • Pharmaceutical composition comprising an extract of pseudolysimachion longifolium and the catalpol derivatives isolated therefrom having antiinflammatory, antiallergic and antiasthmatic activity

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] The preparation of the crude extract of embodiment 1. long leaf panicle

[0067] [0061] 7.9 kg of dried Acarina longifolia was cut into small pieces, mixed with 50 L of methanol, the mixture was stirred at room temperature for 24 hours, and extracted three times with cold water. The extract was filtered with filter paper to remove residue. The filtrate was pooled and concentrated by a rotary evaporator at 55-65° C. under reduced pressure and dried with a freeze dryer to obtain 950.5 g of dry crude extract of Pachyphylla longifolia.

Embodiment 2

[0068] The preparation of embodiment 2. polar solvent and non-polar solvent soluble extract

[0069] [0063] 2-1. Preparation of ethyl acetate soluble fraction

[0070] [0064] Add 10L distilled water to the crude extract obtained in 425g embodiment 1. 1.10 L of ethyl acetate was added thereto through a separatory funnel, and vigorously shaken to separate into an ethyl acetate-soluble layer and a water-soluble layer.

[0071] [0065] The above ethyl acetate soluble layer was concentrated with a rotary evaporator, and dried with a freeze dryer to obtain an ethyl acetate soluble extract.

[0072] [0066] 2-2. Preparation of butanol / water-soluble fraction

[0073] [0067] The water-soluble layer was fractionated by mixing with 10 L of butanol, and finally 144.0 g of n-butanol-soluble extract and water-soluble extract were obtained to be used as samples for the following experiments.

Embodiment 3

[0074] [0068] Example 3. Catalpol derivatives are prepared from the long leaf panicle flower extract

[0075] [0069] 3-1. Preparation of verbascoside (6-O-3,4-dihydroxybenzoyl catalpol)

[0076] [0070] 140.0g n-butanol soluble extract is carried out to silica gel column chromatography (70-230 mesh, 8.5 × 65cm), and eluted with chloroform-methanol mixture (methanol 0-100%, step gradient) to obtain five distillate. Using normal phase silica gel column chromatography (silica gel, 230-400 mesh, 6.0 × 60cm, chloroform-methanol mixture, methanol 10-50% step gradient) to 29.1g fraction 2 (in chloroform-methanol 7 / 3-6 / 4 Between, v / v) carry out repeated column chromatography. Fractions 2-4 were recrystallized from methanol to obtain 14.2 g of verbascoside, 6-O-3,4-dihydroxybenzoyl catalpol. By NMR ( 1 H, 13 C, DEPT, HMQC, HMBC), EI-MS and polarimetry and previously reported methods (Afifi-Yazar F et al., HeIv ChimActa, 63, 1905-7 pages, 1980) confirmed the structure, and the purit...

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Abstract

The present invention relates to a composition comprising an extract of Pseudolysimachion genus plant, and the catalpol derivatives isolated therefrom having anti-inflammatory, antiallergic and anti-asthmatic activity. The extractof Pseudolysimachion genus plantand the catalpol derivatives isolated therefrom shows potent suppressing effect on elevated IgE, IL-4 and IL- 13 levels and eosinophilia in the plasma and BALF, and mucus overproduction in the lung tissues in an OVA-induced asthmatic mouse model. Therefore, it can be used as the therapeutics or functional health food for treating and preventing inflammatory, allergic and asthmatic disease.

Description

technical field [0001] [0001] The present invention relates to compositions comprising extracts of Pseudolysimachion longifolium and catalpol derivatives isolated therefrom having anti-inflammatory, anti-allergic and anti-asthmatic activities. Background technique [0002] [0002] Asthma has been recognized as a complex syndrome occurring in the airways, which exhibits various conditions such as airflow obstruction, acute or chronic inflammation, airway hyperresponsiveness (airwayhyper-responsiveness, AHR) and structural remodeling ( Kumar R.K. Pharmacol. Ther., 91, pp 93-104, 2001). [0003] [0003] It has been reported that allergic inflammation (Allergic inflammation) occurring in the airway plays a key role in the development of asthma, and recently the number of patients suffering from allergic asthma has increased to about 10% of the world's population. %. It has been reported that the number has reached 17 million in the United States until now, and the market size of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/352A61K36/80
CPCA61K31/7048A23L29/30A23V2200/304A23V2200/314Y10S424/81
Inventor 李炯圭吴世亮安竟燮李相龟李重求权玉京金斗英郑革金美真朴普映全桂花
Owner KOREA RES INST OF BIOSCI & BIOTECH
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