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Method for producing fuel ethanol by kudzu root synchronization saccharification fermentation

A technology of synchronous saccharification and fermentation and fuel ethanol, applied in the direction of microorganism-based methods, biofuels, biochemical equipment and methods, etc., can solve the problems of high price and limited food sources, achieve high cost, improve starch utilization rate, energy consumption reduction effect

Inactive Publication Date: 2010-05-12
INST OF PROCESS ENG CHINESE ACAD OF SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a method for producing fuel ethanol by synchronous saccharification and fermentation of kudzu root. The method establishes the optimal fermentation conditions for the production of ethanol by synchronous saccharification and fermentation of kudzu root. Saccharification and fermentation are carried out simultaneously without a separate saccharification process. Utilized by yeast, it can increase the ethanol concentration in the fermentation mash, reduce distillation energy consumption, reduce subsequent wastewater treatment, reduce production costs, and benefit the comprehensive utilization of kudzu root; meanwhile, it solves the problem of limited and expensive grain sources in the production of ethanol from grain raw materials problem

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: get Radix Puerariae 30g, cut into small sections, then mince into fragments of about 0.5-1.0cm, Radix Puerariae and tap water are mixed with tap water in a ratio of 3:1 by mass, then add 6mol / L HCl to adjust the pH to 4.5, when the material temperature is 45°C, add cellulase (1.5U / g kudzu root), keep warm for 20 minutes; then heat up to 90°C, add amylase and cook (5.0U / g kudzu root) for 40 minutes, then cool to 30°C, Under sterile conditions, add glucoamylase (20U / g kudzu root), 0.25% dry yeast, 0.1% (NH 4 ) 2 SO 4 , 0.1% KH 2 PO 4 , then fermented at 35°C, after 72 hours, take the fermented mash and distill it to obtain ethanol; measure the ethanol content in the fermented mash; obtain 0.091g ethanol / g kudzu root, 6.549g ethanol / 100g fermented product, and the starch utilization rate is 97.8%.

Embodiment 2

[0024] Embodiment 2: Get 30 g of kudzu root, cut into small sections, then mince into pieces of about 0.5-1.0 cm, kudzu root and tap water are mixed with tap water in a ratio of 1:1 by mass, and 6mol / L HCl is added to adjust the pH to 4.0 , when the material temperature is 45°C, heat preservation treatment for 10min; then when the material temperature rises to 90°C, add amylase to cook (2.0U / g kudzu root) for 40min, then cool to 30°C, under sterile conditions, add glucoamylase at the same time (65U / g kudzu root), 0.25% dry yeast, 0.05% (NH 4 ) 2 SO 4 , 0.05% KH 2 PO 4 , and then fermented at 35° C., and after 48 hours, the fermented mash was taken to distill ethanol, and the ethanol content in the fermented mash was measured. Obtain 0.071g ethanol / g kudzu root, 5.065g ethanol / 100g fermented product, starch utilization rate is 67.2%.

Embodiment 3

[0025] Embodiment 3: Get 30g of kudzu root, cut into small sections, then mince into pieces of about 0.5-1.0cm, kudzu root and tap water are mixed with tap water in a ratio of 6:1 by mass, and 6mol / L HCl is added to adjust the pH to 4.3 , when the material temperature is 48°C, add cellulase (1.5U / g kudzu root), keep warm for 20min enzyme treatment; then add amylase to cook (5.0U / g kudzu root) for 40min when the material temperature rises to 70°C, and then cool to 32°C, under sterile conditions, add glucoamylase (20U / g kudzu root), 0.10% dry yeast, 0.1% (NH 4 ) 2 8O 4 , 0.1% KH 2 PO 4 , and then fermented at 35° C., and after 60 hours, the fermented mash was taken to distill ethanol, and the ethanol content in the fermented mash was measured. Obtained 0.076g ethanol / g kudzu root, 6.098g ethanol / 100g fermented product, and starch utilization rate was 83.1%.

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Abstract

The invention relates to a method for production of fuel ethanol trough synchronous saccharification and fermentation, which comprises the following steps: Kudzu roots are crushed; water mixed materials are added into the Kudzu roots; HC1 is added into the Kudzu roots; PH value of the Kudzu roots is adjusted; temperature of the Kudzu roots is rose to 45 to 50 DEG C; cellulases are added into the Kudzu roots for enzyme treatment; the temperature of the Kudzu roots is rose to 70 to 90 DEG C again, and alpha-amylases are added into the Kudzu roots for cooking; the temperature of the Kudzu roots is cooled to 30 to 35 DEG C, and maltogenic amylases, (NH4)2SO4, KH2PO4 and yeasts after activation are simultaneously added into the Kudzu roots under aseptic condition for anaerobic fermentation by 48 to 84 hours; ethanol generated by fermentation is distilled and collected. The invention establishes an optimum fermentation condition for production of the fuel ethanol trough synchronous saccharification and fermentation of the Kudzu roots; saccharification and fermentation are carried out simultaneously, and no independent saccharification process is needed; glucoses generated by saccharification is utilized by the yeasts at any moment, thereby the invention has the advantages of capability of improving ethanol concentration in beers, reduction of energy consumption of distillation, reduction of treatment of subsequent waste water, reduction of production cost and favorability of comprehensive utilization of the Kudzu roots, and solves the problems of limited grain sources and expensive price when grain raw materials are used for producing ethanol.

Description

technical field [0001] The method belongs to the utilization field of kudzu root resources, in particular to a method for synchronously saccharifying and fermenting kudzu root as a raw material to produce fuel ethanol. Background technique [0002] At present, my country's fuel ethanol fermentation production mainly uses corn, rice, wheat and other grain crops as raw materials. During the "Tenth Five-Year Plan" period, my country also built a certain scale of fuel ethanol demonstration projects using corn as raw materials in the northern region. However, my country has a large population and little land, shortage of arable land resources, and huge pressure on food production. The development space of fuel ethanol that uses only food (mainly corn and aged rice for combat preparation) as raw materials is greatly limited, and it is difficult to support my country's fuel ethanol industry for a long time. It can be seen that to vigorously develop fuel ethanol in our country, we mu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/06C10L1/02C12R1/85
CPCY02E50/17Y02E50/16Y02E50/10
Inventor 陈洪章汪卫东付小果
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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