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Method for producing fuel ethyl alcohol with sweet potato solid state synchronous diastatic fermentation

A technology for synchronous saccharification and fermentation and fuel ethanol, applied in the direction of microorganism-based methods, biofuels, biochemical equipment and methods, etc., can solve the problems of high prices and limited food sources, achieve high costs, facilitate comprehensive utilization, and reduce The effect of production costs

Inactive Publication Date: 2008-08-20
INST OF PROCESS ENG CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for the production of fuel ethanol by solid-state synchronous saccharification and fermentation of sweet potatoes. Saccharification and fermentation are carried out synchronously without a separate saccharification process. The glucose produced by saccharification can be used by yeast at any time, and it is also beneficial to prevent bacterial contamination while releasing product inhibition. In addition, the ethanol production process by solid-state fermentation only needs to add a small amount of water, which can increase the concentration of ethanol in the fermented mash, reduce the energy consumption of distillation, reduce the treatment of follow-up waste water, reduce production costs, and help the comprehensive utilization of sweet potatoes; The problem of limited and expensive grain sources in the production of ethanol from grain raw materials

Method used

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Examples

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Effect test

Embodiment 1

[0018] Embodiment 1: Wash sweet potatoes and mince them into fragments of about 0.5-1.0 cm, put them into a cooking pot, add tap water and mix well according to the ratio of sweet potatoes to tap water mass ratio of 1:1, heat up to 90°C, Add α-amylase at the ratio of 5U amylase per gram of sweet potato and cook for 25 minutes; cool the cooked sweet potato material to about 30-35°C, and add glucoamylase at the ratio of 100U glucoamylase per gram of sweet potato at the same time under sterile conditions and 0.1% ammonium sulfate, 0.1% potassium dihydrogen phosphate and 3% activated yeast, fermented at 30-35° C. for 72 hours, distilled and collected the ethanol produced by fermentation, and the ethanol concentration was 8.21%.

Embodiment 2

[0019] Embodiment 2: Wash the sweet potatoes and grind them into fragments of about 0.5-1.0 cm, put them into a cooking pot, add tap water and mix well according to the ratio of sweet potatoes to tap water mass ratio of 2:1, heat up to 85°C, Add 7.5U amylase to one gram of sweet potato and add α-amylase to cook for 30 minutes; cool the cooked sweet potato material to about 35°C, and add glucoamylase and 0.15% ammonium sulfate, 0.15% potassium dihydrogen phosphate and 4% activated yeast are fermented at 30-35°C for 60 hours, and the ethanol produced by fermentation is collected by distillation, and the ethanol concentration is 9.37%.

Embodiment 3

[0020] Embodiment 3: Wash sweet potatoes and mince them into fragments of about 0.5-1.0 cm, put them into a cooking pot, add tap water and mix well according to the ratio of sweet potatoes to tap water mass ratio of 3:1, heat up to 85°C, Add α-amylase at the ratio of 10U amylase per gram of sweet potato and cook for 40 minutes; cool the cooked sweet potato material to about 35°C, and add glucoamylase and 0.1 % ammonium sulfate, 0.15% potassium dihydrogen phosphate and 5% activated yeast, ferment for 60 hours at 30-35° C., distill and collect the ethanol produced by fermentation, and the ethanol concentration is 11.90%.

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Abstract

The invention discloses a sweet potato solid synchronous saccharification and fermentation method for producing fuel ethanol. The main process is that: sweet potato is smashed, the material is stirred by adding water, the temperature is increased to 70 DEG C to 90 DEG C, Alpha-amylase is added for stewing and liquification; the cooling is carried out till 30 DEG C to 35 DEG C, glucoamylase, ammonium sulfate, potassium dihydrogen phosphate and activated yeast are added simultaneously under sterile condition for 48 to 84 hours of fermentation, and then the ethanol generated by fermentation is distilled and collected. The saccharification and fermentation of the invention are carried out synchronously without the separate saccharification process, the glucose generated by saccharification can be utilized by yeast at any time, which can not only eliminate the inhibition of the products, but also is beneficial for the prevention of bacteria contamination; in addition, by adopting the solid fermentation technique for producing the fuel ethanol, only small amount of water needs to be added, which can improve the concentration of ethanol in the fermented mash, reduce the energy consumption of distillation, reduce the subsequent treatment of waste water, reduce production cost and be conductive to the comprehensive utilization of sweet potato.

Description

technical field [0001] The invention belongs to the field of utilization of agricultural resources, in particular to a method for producing fuel ethanol by solid-state synchronous saccharification and fermentation using sweet potatoes as raw materials. Background technique [0002] With the rapid development of human society, the crisis of energy and resources has become increasingly prominent, and the conversion of fuel ethanol from biomass raw materials is a hot spot in the field of high-tech research and industrialization in the world. Each country produces bioethanol with different raw materials according to the actual situation. Considering my country's national conditions and biological resources, and in accordance with the principle of "developing fuel ethanol without competing with others for food and land", a feasible plan for developing biomass fuel ethanol is to selectively grow high-yield food in some areas that are not suitable for planting For crop areas, adapt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/06C12R1/645
CPCY02E50/16Y02E50/17Y02E50/10
Inventor 陈洪章付小果汪卫东
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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