Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for producing fuel ethanol by kudzu root fermentation

A fuel ethanol and ethanol technology, applied in the direction of microorganism-based methods, biofuels, biochemical equipment and methods, etc., can solve the problems of limited food sources and high prices, and achieve improved starch utilization, reduced energy, and increased ethanol concentration Effect

Inactive Publication Date: 2009-08-05
INST OF PROCESS ENG CHINESE ACAD OF SCI
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for kudzu root fermentation to produce fuel ethanol, and at the same time solve the problem of limited grain sources and high prices in the production of ethanol from grain raw materials

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Mix Pueraria root (dry matter) and tap water with tap water in a ratio of 1:3 by mass, then add 6 mol / L HCl to adjust the pH. When the material temperature is 45°C, add cellulase (1.5U / g kudzu root) and keep warm for 20 minutes; then heat up to 90°C and add amylase to cook (5.0U / g kudzu root) for 40 minutes, then cool to 58°C , add glucoamylase (20U) for saccharification treatment for 25 minutes, add dry yeast, ammonium sulfate and potassium dihydrogen phosphate under aseptic conditions, and then ferment at 35°C, take the fermented mash and distill it to obtain ethanol after 72 hours;

[0020] The amount of the ammonium sulfate, potassium dihydrogen phosphate and yeast added is: add 0.1g ammonium sulfate, 0.1g potassium dihydrogen phosphate and 0.025g active dry yeast for every 100 grams of kudzu root material;

[0021] The content of ethanol in the fermented mash was measured; 0.090g ethanol / g kudzu root, 6.54g ethanol / 100g fermented product were obtained, a...

Embodiment 2

[0022] Example 2: Add tap water to Pueraria root (dry matter) and tap water that have been cleaned and crushed to about 1.2-1.5 mm and mix well. Add tap water to mix the pueraria root (dry matter) and tap water in a ratio of 1:2 by mass, and then add 6mol / L HCl to adjust the pH When the temperature is 4.3, add cellulase (1.5U / g kudzu root) when the material temperature is 45°C, keep it warm for enzyme treatment for 20min; then heat up to 90°C and add amylase to cook (5.0U / g kudzu root) for 40min, then cool to 58°C , add glucoamylase (20U) for saccharification treatment for 25 minutes, add dry yeast, ammonium sulfate and potassium dihydrogen phosphate under aseptic conditions, and then ferment at 35°C, take the fermented mash and distill it to obtain ethanol after 60 hours;

[0023] The amount of the ammonium sulfate, potassium dihydrogen phosphate and yeast added is: add 0.1g ammonium sulfate, 0.1g potassium dihydrogen phosphate and 0.020g active dry yeast for every 100 grams o...

Embodiment 3

[0025] Example 3: Mix Pueraria root (dry matter) and tap water with tap water to a ratio of 1:3 by mass, then add 6 mol / L HCl to adjust the pH 4.3, when the material temperature is 45°C, add cellulase (3.5U / g kudzu root), keep warm for 25 minutes; then heat up to 80°C, add amylase to cook (7.0U / g kudzu root) for 40 minutes, and then cool to 58°C , adding glucoamylase (20U) for saccharification treatment for 25 minutes, adding dry yeast, ammonium sulfate and potassium dihydrogen phosphate under aseptic conditions, and then fermenting at 35°C. After 48 hours, take the fermented mash and distill it to obtain ethanol;

[0026] The amount of the ammonium sulfate, potassium dihydrogen phosphate and yeast added is: add 0.1g ammonium sulfate, 0.1g potassium dihydrogen phosphate and 0.025g active dry yeast for every 100 grams of kudzu root material;

[0027] Determination of ethanol content in fermented mash; 0.071g ethanol / g kudzu root, 5.065g ethanol / 100g fermented product were obtai...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for producing fuel ethanol through pueraria lobata fermentation, which comprises the following steps: (1) crushing, water adding and stirring, cellulose treatment, water-heat treatment and saccharification treatment are carried out on pueraria lobata raw material to obtain sugar solution; (2) ammonium sulphate, dipotassium hydrogen phosphate and yeast are added into the sugar solution for anaerobic fermentation for 48-72h at the temperature ranging from 30 DEG C to 35 DEG C to produce ethanol; and (3) fermentation broth is distilled to obtain the ethanol. The method takes the pueraria lobata which is drought-enduring and leanness-enduring and can grow on hilly lands and bare lands which are unfavorable for crops growing as the raw material for producing the fuel ethanol, provides a new direction for the raw materials of producing the fuel ethanol, solves the problem that the fuel ethanol is produced by corps which have limited resource and high price, and provides a road to riches for farmers in mountain areas to increase yield and income.

Description

technical field [0001] The method belongs to the utilization field of kudzu root resources, and particularly relates to a method for producing fuel ethanol by fermenting kudzu root as a raw material. Background technique [0002] Radix Pueraria (Radix Pueraria) is the underground root of the Pueraria genus. It is a perennial leguminous vine. It was first recorded in "Shen Nong's Materia Medica". Pharmacological effects of medicinal ingredients. [0003] The main components of kudzu root are: starch, cellulose, protein and kudzu root isoflavones, and it also contains a small amount of fat, pectin, tannins and alkaloids. It is reported in the literature that the starch content in dried kudzu root is about 50-60%, the cellulose content is 9-15%, the crude protein is 5-8%, and the isoflavone content is 3-5%. The new kudzu root has a water content of 50-60%, and a starch content of 18.5-27.5%. Moreover, kudzu root is resistant to drought and barrenness. Wild kudzu root is dist...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P7/06C12R1/645
CPCY02E50/17Y02E50/10
Inventor 陈洪章付小果汪卫东
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com