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Protein coded sequence for regulating and controlling temperature and light sensitive nuclear sterility

A coding sequence, nuclear sterility technology, applied in the direction of sugar derivatives, plant peptides, biochemical equipment and methods, etc.

Inactive Publication Date: 2008-05-28
上海旗冰种业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the 1950s, research on hybrid breeding of indica and japonica rice has been carried out at home and abroad. Some scientists in India, Japan, the United States, and the International Rice Research Institute (IRRI) have carried out research on the utilization of rice heterosis, but they have not been applied to production.
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Method used

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  • Protein coded sequence for regulating and controlling temperature and light sensitive nuclear sterility
  • Protein coded sequence for regulating and controlling temperature and light sensitive nuclear sterility
  • Protein coded sequence for regulating and controlling temperature and light sensitive nuclear sterility

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1: Obtaining and morphological observation of osms4 mutant plants

[0047] This mutant was used in our laboratory 60 Japonica rice 9522 seeds were induced by Coγ-rays at a dose of 280Gy. A male sterile mutant in the mutagenized F2 generation was backcrossed for three generations, and a stable genetic mutant osms4 regulated by a recessive single gene was obtained. All plant materials were planted at the experimental base of Shanghai Academy of Agricultural Sciences. The osms4 mutant was backcrossed with japonica rice 9522, all the F1 generations were fertile, and segregation occurred in the self-crossed F2 generation, of which 189 were normal plants and 55 were mutant plants, and the ratio of normal plants to mutant plants was close to 3:1 (x 2 =0.79□□× 0.05 3.84), indicating that the phenotype of the male sterile mutant was caused by a mutation of a single nuclear gene.

[0048] Morphological observation of osms4 mutant plants. As shown in Figure 1, the ...

Embodiment 2

[0049] Example 2: Research on the relationship between osms4 mutant physicality and temperature and light environment

[0050] 1. Field experiment

[0051] Through the observation of the fertility transition law of osms4 for three consecutive years from 2005 to 2007 (Shanghai Academy of Agricultural Sciences), it was found that the three-year sterile period and fertile period were basically the same:

[0052] In 2005, from August 20th to August 26th, the plants did not bear fruit, and they were sterile. From August 27th to September 5th, the pollen grains had a high fertility rate. Without bagging, the firmness rate is nearly 100%. From September 2nd to September 5th, the firmness rate of bagging reached a maximum of 60%. After September 6th, the bagging did not firm up.

[0053] In 2006, from August 20th to August 30th, the bagging was not strong. From August 31 to September 2, the ear pollen grains had a high fertility rate, and the bagging was firm. After September 3, the...

Embodiment 3

[0060] Example 3: Location and cloning of the OsMS4 gene

[0061] (1) Positioning groups. The osms4 was crossed with the indica rice line Longtefu B, the F2 generation was obtained by selfing, and the male sterile plants were selected as the positioning population.

[0062] (2) Rice DNA extraction. Using the improved CTAB method. The simple steps are as follows: Take 0.1-0.2 grams (about half a piece) of leaves and put them in a small mortar, add an appropriate amount of liquid nitrogen, grind them to powder immediately, put them into a 2ml centrifuge tube, add 700ul of 1.5xCTAB solution preheated at 100℃ in In a centrifuge tube, mix carefully and place in a 56°C water bath. Take out the centrifuge tube after 20 minutes, add an equal volume of chloroform / isoamyl alcohol, mix vigorously, centrifuge (13000rpm) for 10 minutes, take the supernatant into a new tube, add Mix 900ul of absolute ethanol and place at -20°C for more than half an hour. The precipitated DNA was centrif...

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Abstract

Provided is a protein coding sequence of regulating thermo-photo sensitive male sterile, which belongs to the field of gene engineering. The invention discloses a separated DNA molecule, including poly peptide nucleotide sequence with OsMS4 protein activation of rice of the code. The sequence has peptide of the amino acid sequence shown by the SEQ ID NO.2 and the nucleotide sequence from 1 nt to 798 nt shown by the SEQ ID NO.1. The invention has evident effect on detecting and utilizing rice thermo-photo sensitive male sterile. The invention can generate new male sterile line, which is used to produce hybrid, develop new type of two-line hybrid rice, select recurrently and create gene pool. Thereby the invention has a very important application to agricultural production.

Description

technical field [0001] The present invention relates to a protein coding sequence in the technical field of genetic engineering, in particular to a protein coding sequence regulating temperature and photosensitive sterility. Background technique [0002] Rice is one of the most important food crops for human beings, and about half of the world's population uses rice as the main food. China's rice cultivation area accounts for about 23% of the world's total, and its rice output accounts for about 37% of the world's total. The sown area of ​​rice in China accounts for 30% of the sown area of ​​grain crops in the country, but the total output accounts for more than 42% of the grain crop output, and the yield per unit area is 45.17% higher than that of the entire grain crop on average. The widespread application of breeding and heterosis has played a pivotal role. [0003] The phenomenon of heterosis in rice was proposed by Jones (1926). Since the 1950s, indica and japonica r...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415C12N15/79C07H21/04
Inventor 张大兵张辉梁婉琪袁政
Owner 上海旗冰种业科技有限公司
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