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Osteosporosis-resistance chinese medicine extract as well as extracting technique and usage of active component

A technology of anti-osteoporosis and extraction process, which is applied in the field of osteoporosis treatment, and achieves the effects of simple process, low production cost and convenient operation

Inactive Publication Date: 2008-05-21
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In addition to my own research, there is no other report on the extraction of extracts and active ingredients with anti-osteoporosis activity from M. pilosula at present. technology filing invention application

Method used

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  • Osteosporosis-resistance chinese medicine extract as well as extracting technique and usage of active component
  • Osteosporosis-resistance chinese medicine extract as well as extracting technique and usage of active component
  • Osteosporosis-resistance chinese medicine extract as well as extracting technique and usage of active component

Examples

Experimental program
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Effect test

example 1

[0045] Example 1: After the water extract passes through the macroporous resin column, the preparation method of the 90% ethanol eluate: 10 grams of Spongelomangi, pulverize into a coarse powder of about 100 to 200, add 100ml of water to reflux and extract twice, each time for 1 hour , the obtained solvent is freeze-dried or dried under reduced pressure to obtain 3.6g of extract, which is dissolved in hot water and passed through a macroporous adsorption resin column, and the eluate containing 30-90% alcohol is obtained by eluting with a water-alcohol mixed solvent .

example 2

[0046] Example 2: 1. of the preparation method of the total saponins of Saccharomyces spp.: 100 grams of the rhizomes of S. syringae, be ground into a coarse powder of about 100-200 mesh, add 600ml of water to reflux and extract twice, each time for 2 hours, and concentrate the extract under reduced pressure to a small size. volume, first remove the fat-soluble components through chloroform extraction, and then extract with n-butanol to obtain 26.9 grams of total saponins of M. ②: 100 grams of the rhizome of Spongeonia sativa, crushed into a coarse powder of about 100-200 mesh, add 600 ml of 75% ethanol to reflux and extract twice, each time for 2 hours, the filtrate is decompressed to recover the ethanol, and the extract obtained is dispersed with a small amount of water , first remove the fat-soluble components through chloroform extraction, and then extract with n-butanol to obtain 36.9 grams of total saponins of M. ③: 100 grams of the rhizome of Spongeonia sativa, crushed ...

example

[0072] Example: as shown in Figure 1

[0073] Part of 80 g of 90% ethanol was subjected to silica gel column chromatography and washed with CHCl 3 - MeOH gradient elution (99:1-1:99), 6 fractions (Fr.1-Fr.6) were obtained, fractions 2, 3, 4, 5, and 6 were subjected to repeated silica gel column chromatography, reverse phase Thin-layer preparation, macroporous resin column chromatography, recrystallization and other methods obtained 28 compounds, of which 21 were confirmed to have anti-osteoporosis activity, respectively 1: diospongiisn A (diospongiisn A); 2: cotton diospongiisn B; 3: diospongiisn C; 4: piperitol; 5: sesaminone; 6: (+)-syringaresinol; 7: spongipregnoloside A; glycoside C (spongipregnoloside C); 9: spongipregnoloside D (spongipregnoloside D); 10: spongiosides E; 11: dioscin; 12: pregna-5, 16-diene-1β-o1-20-one- 3-O-[α-L-rhamnopyranosyl-(1→2)]-[α-L-rhamnopyranosyl-(1→4)]-β-D-glucopyranoside; 13: dioscin; 14: prosapogenin A ;15:gracillin;16:hypoglaucin G;17:met...

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Abstract

The invention discloses a Chinese traditional medicine sevenlobed yam rhizome osteoporosis-preventing activity extractive and the extraction process of active ingredient as well as the use for curing osteoporosis. The process uses Chinese traditional medicine of sevenlobed yam rhizome and the dried tuber of freshly collected sevenlobed yam rhizome as raw materials, and adopts solvent extraction method, resin absorption method, reserve-phase pole chromatography and opposite phase pole chromatography to prepare the extractive. Moreover the process uses sevenlobed yam rhizome activity extractive as raw material, and adopts reserve-phase pole chromatography, opposite phase pole chromatography, reserve-phase preparation thin layer chromatography, opposite phase preparation thin layer chromatography, crystallization method and preparation liquid phase chromatogram for separation to obtain each monomer composite. The sevenlobed yam rhizome extractive and partial monomer composites obtained from the process are applied in preparing the drug of preventing osteoporosis.

Description

technical field [0001] The invention relates to an extraction process of an extract with anti-osteoporosis activity obtained from a natural plant, specifically, the traditional Chinese medicine Spongeba sativa, and an active component and its use for treating osteoporosis. Background technique [0002] Osteoporosis (Oste0Porosis referred to as OP) is a systemic and systemic bone disease characterized by decreased bone mass and microstructural destruction of bone tissue leading to increased bone fragility and fracture risk. It is also a systemic disease of bone metabolism disorder with low bone density as the main symptom. The international medical community has listed osteoporosis, diabetes, and cardiovascular disease as the three major diseases that seriously endanger the health of the elderly. [0003] Mian 萆 薢: also known as white 萆 薢, Chuan 萆 薢, cotton 萆 薢. It is the dry rhizome of the plant Spongeonia sativa. The plant sources of Spongelus are Dioscorea spongiosa J.Q...

Claims

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Application Information

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IPC IPC(8): A61K36/8945A61K31/7048A61P19/10C07B63/00C07C29/76C07C33/26C07D407/14C07D493/04C07H1/08C07J7/00C07J9/00C07J71/00A61K125/00
Inventor 殷军刘阳刘志惠王卓王勇
Owner SHENYANG PHARMA UNIVERSITY
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