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Method for regenerating plant strain using soybean cotyledonary node

A technology of cotyledon nodes and soybeans, applied in the field of agricultural production, can solve problems such as the confusion of medium application and the influence of plant regeneration frequency

Inactive Publication Date: 2008-05-14
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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Problems solved by technology

[0003] Although the source of explants in the soybean plant regeneration system is relatively clear, the disinfection of seeds in the tissue culture process and the application of various media in the process of organogenesis are still relatively confusing, which also has a great impact on the frequency of plant regeneration

Method used

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Embodiment Construction

[0015] The invention is applied in the subject of "genetic transformation of soybean high vitamin E synthesis gene" of Northeast Institute of Geography and Agricultural Ecology, Chinese Academy of Sciences. Adopt the present invention to carry out high vitamin E synthetic gene genetic transformation research on varieties such as Heinong 35, Hefeng 35, Hefeng 25 and Suinong 14, which are more used in production, in the subject implementation process, for the completion and high-level results of this subject Vitamin E soybean breeding has played an effective technical support. The soybean cotyledon node genotype screening results are listed below:

[0016] Genotype Screening of Soybean Cotyledon Node

[0017] Preface

[0018] According to the genotype screening results of soybean cotyledon nodes, it can be seen that Hefeng 25 has the highest cluster bud differentiation rate of 94.20%, followed by Heinong 35 with a cluster bud diffe...

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Abstract

The invention relates to a method using the cotyledon node of soybeans as explant to perform soybean organogenesis and plant regeneration, belonging to the technical field of agricultural production. The invention is characterized in that based on the traditional tissue culture, the cotyledon node of soybeans is used as explant to perfect the regeneration system of organogenesis means. The invention has the advantages that based on the selection of effective disinfection technology for soybean seeds, the improvement and the optimization of various medium compositions is outstanding; through the verification of the tissue culture of conventional soybean varieties, the induction, the elongation and the rooting of multiple shoot are effectively promoted; the quality of aseptic seedlings and the differentiation rate of the multiple shoot are improved; the organogenesis and plant regeneration process of the cotyledon node of majority varieties are perfectly realized; solid technical support is provided for the genetic transformation and the molecular improvement of soybeans; and the invention has important practical significance to the effective breeding and health production of the soybeans in China.

Description

technical field [0001] The invention belongs to the technical field of agricultural production. It is a method for soybean organogenesis and plant regeneration using soybean cotyledon nodes as explants. technical background [0002] Soybean plant regeneration system is a prerequisite for the realization of soybean genetic transformation. A good soybean plant regeneration system should have high-efficiency seed disinfection technology, suitable seed germination medium, cluster bud induction medium, elongation medium, rooting medium and necessary sterile seedling acclimatization and cultivation techniques. Since the early 1980s, people have selected immature embryos, immature embryo cotyledons or hypocotyls as explants, and established soybean somatic embryogenesis and organogenesis regeneration systems with abnormally high concentrations of plant growth regulators. Subsequently, a higher frequency of regenerated plants derived from adventitious buds was successively obtaine...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04A01G31/00
Inventor 潘相文李艳华张秋英王国栋邢海军张凤芸
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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