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Low-phosphor culture medium for chlorella heterotrophy culture

A heterotrophic culture and medium technology, applied in the direction of single-cell algae, etc., can solve the problems of unstudied chlorella's phosphorus absorption characteristics, resource waste environment, excessive phosphorus content, etc., achieve significant economic and environmental benefits, eliminate phosphorus Residue, reduce the effect of input

Inactive Publication Date: 2008-05-07
SHANGHAI JIAO TONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its deficiency is: (1) under the condition of heterotrophic culture, Chlorella does not study the absorption characteristics of phosphorus, and the influence of carbon-to-phosphorus ratio on the growth and biomass of Chlorella; (2) According to the technical scheme provided in the paper, Basal The phosphorus content in the medium is seriously excessive, and a relatively high concentration of phosphorus remains in the medium after the culture is over, resulting in waste of resources and environmental pollution

Method used

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  • Low-phosphor culture medium for chlorella heterotrophy culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Low phosphorus medium formula for heterotrophic culture of Chlorella (mg / l): Glucose H 2 O 5000, KH 2 PO 4 10.KNO 3 300, MgSO 4 ·7H 2 O 1000, EDTA 500, H 3 BO 3 114.2, CaCl 2 2H 2 O 111, FeSO 4 ·7H 2 O49.8, ZnSO 4 ·7H 2 O 88.2, MnCl 2 4H 2 O 14.2, MoO 3 7.1, GuSO 4 ·5H 2 O 15.7, Co(NO 3 ) 2 ·6H 2 O4.9.

[0027] It is used for heterotrophic batch culture of chlorella in a 19-l fermenter. The culture medium is prepared by dissolving in tap water, with a filling capacity of 12l, sterilized at 121°C for 20 minutes, and cooled to below 30°C. Culture conditions: inoculum size 8% (v / v), pH 6.5, temperature 28°C, stirring speed is related to the oxygen saturation concentration of the culture solution, and the oxygen saturation concentration is controlled to be greater than 50%.

[0028] 5g initial glucose / l, 2.28mg initial phosphorus phosphate / l, Chlorella grows rapidly, and the maximum cell concentration is 2.6g / l, indicating that the phosphorus concen...

Embodiment 2

[0030] Low phosphorus medium formula for heterotrophic culture of Chlorella (mg / l): Glucose H 2 O 10000, KH 2 PO 4 100, KNO 3 1250, MgSO 4 ·7H 2 O 1000, EDTA 500, H 3 BO 3 114.2, CaCl 2 2H 2 O 111, FeSO 4 ·7H 2 O49.8, ZnSO 4 ·7H 2 O 88.2, MnCl 2 4H 2 O 14.2, MoO 3 7.1, CuSO 4 ·5H 2 O 15.7, Co(NO 3 ) 2 ·6H 2 O4.9.

[0031] It is used for batch heterotrophic culture of chlorella in 19-l fermenter, the medium is prepared by dissolving in tap water, the filling capacity is 12l, sterilized at 121°C for 20 minutes, and cooled to below 30°C. Culture conditions: inoculum size 10% (v / v), pH 6.5, temperature 28°C, stirring speed is related to the oxygen saturation concentration of the culture solution, and the oxygen saturation concentration is controlled to be greater than 50%.

[0032] After culturing for about 48 hours, the cell concentration of Chlorella reached the maximum value, and the maximum cell concentration was 5.5g / l, indicating that the phosphorus ...

Embodiment 3

[0034] Low phosphorus medium formula for heterotrophic culture of Chlorella (mg / l): Glucose H 2 O 40000, KH 2 PO 4 100, KNO 3 5000, MgSO 4 ·7H 2 O 1000, EDTA 500, H 3 BO 3 114.2, CaCl 2 2H 2 O 111, FeSO 4 ·7H 2 O49.8, ZnSO 4 ·7H 2 O 88.2, MnCl 2 4H 2 O 14.2, MoO 3 7.1, CuSO 4 ·5H 2 O 15.7, Co(NO 3 ) 2 ·6H 2 O4.9.

[0035] It is used for batch heterotrophic culture of chlorella in 19-l fermenter, the medium is prepared by dissolving in tap water, the filling capacity is 12l, sterilized at 121°C for 20 minutes, and cooled to below 30°C. Culture conditions: inoculum size 10% (v / v), pH 6.5, temperature 28°C, stirring speed is related to the oxygen saturation concentration of the culture solution, and the oxygen saturation concentration is controlled to be greater than 50%.

[0036] After culturing for about 84 hours, the cell concentration of Chlorella reached the maximum value, and the maximum cell concentration was 21g / l, indicating that the phosphorus c...

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Abstract

The invention relates to a low phosphorous culture medium for heterotrophic nutrition culturing chlorella, belonging to technical field of biology. The invention adopts the components and the concentration content of the components (mg / l) as following: Glucose x H2O is 5000 to 50000; KH2PO4 is 10 to 1000; KNO3 is 300 to 7500; MgSO4 x 7H2O is 1000 to 1250; EDTA is 500 to 625; H3BO3 is 114.2 to 142.75; CaCl2 x 2H2O is 111 to 138.75; FeSO4 x 7H2O is 49.8 to 62.25; ZnSO4 x 7H2O is 88.2 to 110.25; MnCl2 x 4H2O is 14.2 to 17.75; MoO3 is 7.1 to 8.875; CuSO4 x 5H2O is 15.7 to 19.625, and Co(NO3)2 x 6H2O is 4.9 to 6.125. The invention can be used in culturing chlorella and the dosage of KH2PO4 is only 1.6% to 16% to the former Basal prescription, but the inoculum concentration, the specific growth rate, the yield of the biomass, the percent conversion of biomass to glucose, the growing trend and the culturing time are all equal to the former Basal cultivation.

Description

technical field [0001] The invention relates to a microalgae culture medium in the technical field of bioengineering, in particular to a low-phosphorus culture medium for chlorella heterotrophic culture. Background technique [0002] Chlorella is a single-cell microalgae, rich in protein, carotenoids, polysaccharides, vitamins and chlorella growth factors and other nutrients and active ingredients, with anti-oxidation, anti-tumor, improving immunity and preventing heart disease. Physiological functions such as vascular diseases. At present, the cultivation of chlorella is mainly based on the open photoautotrophic cultivation method, and its disadvantages are that it occupies a large area, is affected and limited by natural conditions and seasonal climate, it is difficult to maintain stable yield and quality, slow growth rate, and low biomass ( In the autotrophic culture of Chlorella, the cell concentration is usually 200-600 mg cell dry weight / l), and it is easy to be pollu...

Claims

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Application Information

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IPC IPC(8): C12N1/12
Inventor 史贤明曲春波吴正云施春雷
Owner SHANGHAI JIAO TONG UNIV
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