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Organic preserving solution and its preparation method

An organ preservation solution and double-distilled water technology, applied in the fields of biology and medicine, can solve the problems of inability to reduce perfusion re-injury in organ transplantation, organ transplantation obstacles, etc., and achieve effective resistance to oxygen free radical damage, inhibition of acidosis, and improvement of blood vessels. Effects on endothelial function

Inactive Publication Date: 2008-04-30
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, this kind of preservation solution can not reduce the perfusion re-injury in the process of organ transplantation, and there are certain obstacles to further improving the survival rate after organ transplantation.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Preparation of organ preservation solution (take the preparation of 1000ml as an example):

[0023] Preparation of raw materials: mannitol 10.93g; histidine 4.63g; glutathione 0.92g; sodium glutamate 3.74g; sodium lactate 6.90ml; KCl 1.12g; MgCl 2 2.64g; 0.1mol / L CaCl 2 2.5ml; tetrahydrobiopterin 1.57mg; diazoxide 6.92mg; sufficient double distilled water.

[0024] Preparation Process:

[0025] Specific steps are as follows:

[0026] Dissolve 1.57mg of tetrahydrobiopterin and 6.92mg of diazoxide in 1ml of dimethyl sulfoxide (DMSO) as mother liquor; prepare 0.1mol / L CaCl 2 10ml for later use; add 800ml double distilled water into >1000ml container, then add KC1 1.12g, MgCl 2 2.64g, mannitol 10.93g, histidine 4.63g, glutathione 0.92g, sodium glutamate 3.74g, stir until the color becomes clear; add sodium lactate 6.90ml, stir until the color becomes clear; add 0.1mol / L CaCl 2 2.5ml, stir until the color becomes clear; add the spare mother liquor, stir until the c...

Embodiment 2

[0028] Application of Organ Preservation Solution in Langendoff Isolated Rat Heart Perfusion and Cold Preservation

[0029] Subject: Male SD rats (body weight 230-270g)

[0030] Rats were divided into ① control group: isolated hearts were preserved in Celsior cardioplegia solution, n=8; ②DE+BH 4 Group: The isolated heart was preserved in the organ preservation solution provided in Example 1 (hereinafter referred to as "the organ preservation solution"), n=8.

[0031] Implementation process:

[0032]After the rats were weighed, the thoracotomy was taken out, and the heart was quickly transferred and fixed on the Langendoff perfusion device, and conventional retrograde constant pressure perfusion (76mmHg) was performed with modified K-H solution. The temperature was maintained at 37 °C throughout the perfusion period, and the perfusion fluid was filled with 95% O 2 +5%CO 2 saturation. The heel of the pulmonary artery is cut to allow adequate drainage of the coronary reflux ...

Embodiment 3

[0034] The cardiac function index before and after preservation described in embodiment 2 is measured, and the results are as follows:

[0035] Table 1 Changes of left ventricular end-diastolic pressure (LVEDP) after 8 hours of cold storage

[0036] Group

LVDEP during reperfusion(%of equilibrium)

20min

30min

40min

50min

60min

control

DE+BH 4

462.58±18.25

324.47±19.49 *

451.54±12.01

321.31±11.04 *

469.46±15.47

317.82±12.74 *

475.82±15.66

315.90±16.18 *

482.23±15.63

325.83±15.35 *

[0037] * p<0.05 vs control group. After cold storage for 8 hours, compared with pure Celsior solution, this storage solution can significantly inhibit the increase of LVEDP after storage.

[0038] Table 2 Changes of left ventricular developing pressure (LVDP) after 8 hours of cold storage

[0039] Group

LVDP during reperfusion(%of equilibrium)

20...

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Abstract

The invention belongs to the technology of medical biology and relates to organ preservative fluid and the method for making same. The procedure to prepare 1000ml of the fluid is as follows, tetrahydrobiopterin 1.57mg and diazoxide 6.92mg are dissolved in 1ml of dimethyl sulfoxide to act as mother liquid, CaCl2 of 0.1mol / L is prepared for backup, double distilled water 800ml is charged in a container of 1000ml, KCl 1.12g, MgCl2 2.64g, manna sugar 10.93g, histidine 4.63g, glutathion 0.92g and glutavene 3.74g are sequentially charged and agitated to limpidity, 6.90ml of sodium lactate is charged and agitated to limpidity, CaCl2 2.5ml of 0.1mol / L is charged and agitated to limpidity, and backup liquid and mother liquid are charged and agitated to limpidity. The pH is adjusted to 7.3-7.5, the osmotic pressure is adjusted to 320Osm / L, and the volume of double distilled water is set to 1000ml. The fluid is stored in low temperature of 4 DEG C.

Description

technical field [0001] The invention belongs to the technical fields of biology and medicine, relates to organ preservation in organ transplantation technology, and more specifically relates to an organ preservation solution and a preparation method thereof. Background technique [0002] Organ transplantation is a major advance in human medical science in the 20th century. At present, the total number of transplants in various fields in the world has exceeded 700,000 cases (times), and the longest surviving organ transplant recipient has been nearly half a century. Organ preservation is one of the three technical pillars of organ transplantation. In order to improve the long-term survival rate of patients and alleviate the contradiction of the shortage of donor organs, transplant clinics put forward higher requirements for organ preservation technology. [0003] The University of Wisconsin Solution (UW solution), successfully developed by Professor Belzer of the University...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 张晓明王丽娜朱一苗沈岳良郑鸣之
Owner ZHEJIANG UNIV
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