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Method for separating and preparing corosolicacid in loquat leaf

A technology of corosolic acid and loquat leaves, applied in the field of separation and preparation of corosolic acid from loquat leaves, achieving the effects of efficient separation, good solubility and strong penetration ability

Inactive Publication Date: 2008-03-19
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the separation and purification of corosolic acid in loquat leaves is not yet mature, and only Chen Longsheng et al. have investigated the influence of solvent extraction conditions on the extraction rate of corosolic acid (Chen Longsheng et al., Forestry Chemistry and Industry, 2006, 26 (3), 110)

Method used

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  • Method for separating and preparing corosolicacid in loquat leaf
  • Method for separating and preparing corosolicacid in loquat leaf

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Using loquat leaves produced in Putian, Fujian as raw materials, wash fresh loquat leaves with water, dry (air-dried, oven-dried or sun-dried), crush, and sieve through 20-30 meshes to obtain loquat leaf powder, and put 5.2kg of loquat leaf powder into the extraction In the tank, use 95% edible alcohol 15 times the dry weight of loquat leaves (W / W), extract 3 times at 65°C under the condition of pH value 4, each time for 2 hours, combine the extracts, filter; the filtrate is oxidized with hydrogen Adjust the pH value to 9.0 with sodium, let stand and age at 65°C for 3 hours, filter to remove insoluble matter, add 1 kg of activated carbon to decolorize at 65°C for 1 hour, and filter. The filtrate was adjusted to pH 8.0 with dilute hydrochloric acid aqueous solution, the ethanol concentration reached 50% and the total triterpene acid concentration was 0.5 g / L, wherein the corosolic acid concentration reached 0.2 g / L.

[0037] Next, the filtrate is adsorbed to saturation b...

Embodiment 2

[0040] First put 4.6kg loquat leaf powder into the extraction tank, adopt 80% methanol aqueous solution of 10 times of loquat leaf dry weight (W / W), under the condition of pH value 11.0, extract 3 times at 50 ℃, each 1 hour, The extracts were combined and filtered; the filtrate was left to age at 50°C for 1 hour, filtered to remove insoluble matter, and then 0.9 kg of activated carbon was added to decolorize at 50°C for 0.5 hour, and then filtered. Filtrate is under -0.05~-0.09MPa, 50~75 ℃ of conditions, after decompression recovery part methanol solvent, adjust the pH value of loquat leaf total triterpene acid solution to 11.0 with dilute sulfuric acid aqueous solution, methanol concentration reaches 20% and total The concentration of triterpene acid was 4.0g / L, and the concentration of corosolic acid reached 1.5g / L.

[0041] Then, the filtrate is adsorbed to saturation by D101 macroporous adsorption resin for corosolic acid, and the unadsorbed loquat leaf total triterpene ac...

Embodiment 3

[0044] First put 6.3kg of loquat leaf powder into the extraction tank, use absolute ethanol with 5 times the dry weight of loquat leaves (W / W), under the condition of pH value 7, extract under reflux at 80°C for 2 hours, filter; Adjust the pH value to 12.0 with sodium oxide, stand and age at 70°C for 2 hours, filter to remove insoluble matter, add 1.2kg of activated carbon to decolorize at 70°C for 1.5 hours, filter; the filtrate is at -0.05~-0.09MPa, 50~75°C After recovering part of the ethanol solvent under reduced pressure, adjust the pH value to 12.0 with ethanol aqueous solution containing dilute hydrochloric acid, the ethanol concentration reaches 40% and the total triterpene acid concentration is 10.0g / L, wherein the corosolic acid concentration reaches 5.0g / L.

[0045] Next, the filtrate is adsorbed to saturation by HZ816 macroporous adsorption resin for corosolic acid, and the unadsorbed loquat leaf total triterpene acid residue liquid and 2 times the resin amount ar...

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Abstract

The present invention provides a method of separating and preparing corosolic acid from loquat leaves, which includes the separation and preparation of the total triterpene acid loquat leaf, the purification of the resin concentration of the corosolic acid, the crystallization and purification of the corosolic acid, etc. In the preparation method, the loquat leaves are used as a material, and the total triterpene acid loquat leaf is extracted by applying the aqueous solution of the hydrophilic organic solvent; under the alkaline condition and before cooling, impurities are removed by filtration, and activated carbon is decolored; extracting solution applies macroporous absorptive resin concentration to purify the corosolic acid, and the crude corosolic acid is further crystallized and recrystallized by applying the hydrophilic organic solvent, so that the high-purity corosolic acid crystal is prepared. By measurement, the purity of the white corosolic acid crystal is larger than or equal to ninety eight percent. The preparation technique of the present invention has the advantages of simple technique, high efficiency of separation and purification, easy industrialized production, high purity of the prepared corosolic acid and low production cost, and moreover, the preparation technique of the present invention has the advantages of high efficiency, high availability, long effect, little dosage, little toxicity, little side effect, convenient storage, convenient carrying and convenient administration, etc. of the modern Chinese medicine.

Description

technical field [0001] The invention relates to a green chemical production method for extracting medicinal active ingredients from plants, more specifically to a method for separating and preparing industrially applicable corosolic acid (corosolic acid) with hypoglycemic and anticancer functions from loquat leaves . Background technique [0002] Corosolic acid belongs to pentacyclic triterpene acid compounds and is a derivative of ursolic acid. Compared with ursolic acid, corosolic acid has an α-hydroxyl group on the 2-carbon, commonly known as 2α-hydroxyursolic acid. The schematic diagrams of the chemical structures of the two are as follows: [0003] [0004] Schematic diagram of the chemical structure of corosolic acid [0005] [0006] Schematic diagram of the chemical structure of ursolic acid [0007] Research results in recent years have found that the extract of Lagerstroemia indica has the functions of hypoglycemic, lipid-lowering, anti-tumor and anti-oxi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00A61K36/73
Inventor 陈剑锋
Owner FUZHOU UNIV
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