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Cremastra appendiculata tissue culturing rhizome fungus root seedling raising technique

A rhododendron orchid root and mycorrhizal fungus technology, which is applied in the fields of cultivation, soilless cultivation, biochemical equipment and methods, etc., can solve the problem of synergy research without mycorrhizal fungus multi-bacteria, no breakthrough progress in the application of mycorrhizal fungi, etc. Strains cannot be isolated and other problems

Inactive Publication Date: 2008-03-12
贵州省生物技术研究所
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Problems solved by technology

However, for a long time, no breakthrough has been made in the application of mycorrhizal fungi of Orchidaceae plants that can carry out photosynthesis in adults. The reasons are: one is that the surface disinfection method is mainly used to isolate mycorrhizal fungi, so that some slow-growing strains cannot be separated; Culture system, so that a large number of mycorrhizal bacteria are excluded as pathogenic bacteria
This eventually led to the fact that the bacteria screened by many researchers were not true mycorrhizal fungi, and the application of mycorrhizal fungi became more and more difficult

Method used

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  • Cremastra appendiculata tissue culturing rhizome fungus root seedling raising technique

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Embodiment Construction

[0024] 1. Isolation of mycorrhizal fungi

[0025] Isolation of mycorrhizal fungi by single mycelium separation method, see the patent "Single mycelium separation technology of orchid mycorrhizal fungi", application number: 00610051196.2, publication number: CN1912101. A total of 7 kinds of mycorrhizal fungi were isolated, all of which were Rhizoctonia, code-named Rhizoctonia R1, Rhizoctonia R2, Rhizoctonia R3, Rhizoctonia R4, Rhizoctonia R5, Rhizoctonia R6, Rhizoctonia R7, and the separation ratio was 1:3:1:1:2 :1:5.

[0026] 2. Culture of mycorrhizal bacteria

[0027] Pick the strains from the test tube and transfer them to a 60cm PDA plate, culture at 25°C for 15 days to activate; use a puncher with a diameter of 0.5cm to punch 3 bacteria cakes from the plate, and use the three-point method to inoculate them on a 90cm-diameter PDA plate, 25 Cultivate at ℃ until the colony is almost full of the plate to obtain the solid seed; use a puncher to punch out 5-10 bacteria cakes, ...

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Abstract

The present invention belongs to the technical field of plant tissue culture seedling fungus breeding; the present invention mainly provides the crucial technology method of Cremastra appendiculata tissue culture rhizomorphous fungus breeding. The present invention relates to the methods of separation of mycorrhizal fungi, culture of mycorrhizal fungi, preparation of mixed fungus agent, rhizomorphous stem fungus breeding and tissue culture seedling fungus cultivation. The whole technology simplifies the variety and complexity of arethusa mycorrhizal fungi which closely grasps the nature of the synergistic action of a plurality of fungi and with the method, the phase of rhizomorphous stem is chosen for breeding which greatly shortens the tissue culture time and promotes the survival rate and reduces the cost; the present invention provides a referential novel pattern for the application of arethusa mycorrhizal fungi.

Description

1. Technical field [0001] The invention belongs to the technical field of mycorrhized seedling cultivation of plant tissue culture seedlings 2. Background technology [0002] Rhododendron orchid (Cremastra appendiculata (D.Don) Makino), also known as Mao Cigu and Abacus Seven, is a perennial herb of the family Orchidaceae Mao Cigu. Chinese medicinal materials. Rhododendron orchid first appeared in "Supplement to Materia Medica", and it uses pseudobulbs as medicine. Its rhizome contains mucus and grape mannan, which has the functions of reducing swelling, dispelling stagnation, resolving phlegm, detoxifying, treating carbuncle, scrofula, scrofula, sore throat, Lymph node tuberculosis, snakes, insects, rabies and other effects. Rhododendron has strict requirements on the ecological environment and likes to be cool and humid. At present, it mainly relies on ramets for reproduction, and the reproduction coefficient is extremely low. Generally, a protocorm only grows a new corm...

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Application Information

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IPC IPC(8): A01H4/00C12N1/00A01G31/00
Inventor 朱国胜刘作易毛堂芬桂阳黄永会
Owner 贵州省生物技术研究所
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