Method for long conserving microorganism bacterial

Abstract

The invention relates to a preservation method for microbial strains, and mainly relates to a long-term preservation method for yeast, bacillus and aspergillus strains. The weight ratio of the culture medium: 40-70% of cornmeal, 5-30% of bran, 15-35% of soybean meal powder, the ratio of material to water is 1:0.8-1.2; the culture medium is subpackaged and sterilized: the culture medium is dosed according to the above Mix the materials with water, put them into test tubes, sterilize with high-pressure steam at 1.2atm for 30-45 minutes, and cool them for later use; inoculation culture: insert 1-2 rings of bacteria (0.1-0.2ml) under sterile conditions, and put them in the incubator Cultivate at 26-30°C for 2-3 days; dry storage: dry at 40-45°C for 2-3 days, keep the water content at ≤6%, pack in the refrigerator at 1-4°C or -16°C ~-20 ℃ storage period up to 3 to 5 years. This method requires less investment in equipment, and long-term preservation of strains can be realized in ordinary refrigerators. It has strong practicability, and has the characteristics of simple operation, stability and reliability, low cost and long preservation period.

Description

technical field [0001] The invention relates to a preservation method for microbial strains, and mainly relates to a long-term preservation method for yeast, bacillus and aspergillus strains. Background technique [0002] At present, microbial strains are usually preserved by regular transplantation on inclined planes, liquid paraffin preservation, freeze-drying, liquid nitrogen cryopreservation, and -80°C cryopreservation. Preservation under low temperature conditions can slow down the metabolic activity of microbial strains, inhibit their reproduction speed, achieve the purpose of reducing strain mutations and prolonging the preservation time of strains. [0003] Slope regular transplantation preservation method: usually in a glass test tube with a cotton plug, use less than 1 / 3 tube of sterile solid medium to form a slope, and inoculate the center with a line, and cultivate at 28-30°C for 2 -After 3 days, store in the refrigerator at 4°C. This method is the main preserva...

AI Technical Summary

Problems solved by technology

[0008] The above three methods can preserve microbial strains well. The preservation period is long and the mutation frequency is low. The disadvantages are complex operation, high technical content, high equipment investment and high operating cost. They are only suitable for professional preservation institutions and not suitable for production enterprises. use