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Method for identifying cordyceps sinensis products and uses thereof

A technology of Cordyceps and products, which is applied in the field of identification of Cordyceps products, and can solve the problem that there is no good quality control method for active ingredients

Inactive Publication Date: 2008-02-06
柯传奎 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are still many active ingredients with high content without good quality control methods, such as polysaccharides

Method used

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  • Method for identifying cordyceps sinensis products and uses thereof
  • Method for identifying cordyceps sinensis products and uses thereof
  • Method for identifying cordyceps sinensis products and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Weigh about 1 gram each of natural Cordyceps sinensis, Cordyceps militaris, Cordyceps militaris and artificial Cordyceps pseudo-Cordyceps, add 10 times the weight of water to reflux and extract for 2 hours, and the extract is centrifuged to obtain a supernatant, and the supernatant is added with 4 times the volume of Precipitate with absolute ethanol, and separate the precipitate after standing for 2 hours, and dry the precipitate to obtain the crude polysaccharide of Cordyceps sinensis. Take about 0.02 g of crude polysaccharide, add 2mol / L H 2 SO 4 Solution 2ml, seal, mix and dissolve, react at 125°C for 1 hour, let it cool to room temperature, centrifuge at 3500r / min for 10 minutes, adjust the pH of the supernatant to 7.0 with 4mol / L NaOH aqueous solution, measure 1.0ml of this hydrolyzate before hydrolysis Add 1.0ml each of 0.3mol / L NaOH aqueous solution and 0.5mol / L 1-phenyl-3-methyl-5-pyrazolone methanol solution to the tube, mix well, seal, and react in a water b...

Embodiment 2

[0018] Weigh about 1 gram each of natural Cordyceps sinensis from Qinghai, Sichuan, Yunnan, Tibet and Gansu, add 8 times the weight of water to reflux and extract for 2 hours, and the extract is centrifuged to obtain a supernatant, and the supernatant is added with 4 times the volume of Precipitate with absolute ethanol, and separate the precipitate after standing for 2 hours, and dry the precipitate to obtain the crude polysaccharide of Cordyceps sinensis. Take about 0.02g of crude polysaccharide, add 2ml of 2M trifluoroacetic acid, seal and mix well, react in an oven at 125°C for 1 hour, let it cool to room temperature, and dry the hydrolyzate under nitrogen flow. Add 1ml of methanol, mix well and blow dry under nitrogen flow. Add 1ml of distilled water and 25mg of sodium borohydride powder to the dried sample, mix and react for 2 hours. Acetic acid was added dropwise to stop the reaction until there were no bubbles. Dry the reaction liquid under nitrogen flow, add 0.1% (V...

Embodiment 3

[0022] Weigh about 1 g each of the contents of Jinshuibao Capsules, Bailing Capsules, Ningxinbao Capsules, and Trichosporum mycelia powder produced by Zhejiang Cifu Pharmaceutical Co., Ltd., add 15 ml of water, and extract by ultrasonic at 80 degrees 2 hours. The extract is centrifuged to obtain a supernatant, which is precipitated by adding 4 times the volume of absolute ethanol to the supernatant, left to stand for 2 hours, separated to obtain a precipitate, and the precipitate is dried to obtain the crude polysaccharide of Cordyceps sinensis. Take about 0.02 g of crude polysaccharide, add 2mol / L H 2 SO 4Solution 2ml, seal, mix and dissolve, react at 125°C for 1 hour, let it cool to room temperature, centrifuge at 3500r / min for 10 minutes, adjust the pH of the supernatant to 7.0 with 4mol / L NaOH aqueous solution, measure 1.0ml of this hydrolyzate before hydrolysis Add 1.0ml each of 0.3mol / L NaOH aqueous solution and 0.5mol / L 1-phenyl-3-methyl-5-pyrazolone methanol solution...

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Abstract

The present invention provides a cordyceps product verifying method and application. The analysis on amylase and monosaccharose composition of different cordyceps products shows great difference; thereby verifying the types of cordyceps by analyzing the amylase and monosaccharose constitutes of cordyceps products. The present invention is suitable for natural cordyceps, artificial fermentation cordyceps and artificial fake cordyceps. As for a certain cordyceps product, the analysis of the amylase and monosaccharose composition is carried on the samples from the different batch produced or different production regions, and the result shows no remarkable difference, therefore, the method provided by the present invention can be used to verify batch stability of cordyceps products to control the quality of products.

Description

(1) Technical field [0001] The invention relates to a method for identifying cordyceps products and its application. (2) Background technology [0002] The Cordyceps described in this case refers to the natural Cordyceps genus, artificial pseudo-Cordyceps or Cordyceps products fermented by the strains of the genus Cordyceps. [0003] According to incomplete statistics, there may be more than 300 species of Cordyceps, and as many as 497 species can be retrieved from the fungal name database provided by CABI Bioscience in the UK, but some of them are homonyms. At present, there are about 70 species distributed in our country. Among them, the most famous and commonly recognized by the common people is Cordyceps sinensis, whose Latin name is Cordyceps sinensis (Berk.) Sacc. Cordyceps sinensis recorded in ancient Chinese medicine generally refers to Cordyceps sinensis. In addition, the common ones are Cordyceps militaris, Cordyceps japonica, Cordyceps guni and so on. In addit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/02G01N30/06
Inventor 李艳伟柴黎燕欧阳小军张萍傅惠英柯传奎杨勇杰
Owner 柯传奎
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