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Micro-ecological bacterial preparation for killing soil nematodes and its preparation method

A technology of soil nematodes and bacteria preparations, applied in the direction of nematicides, biocides, biocides, etc., can solve the problem of high density

Inactive Publication Date: 2008-01-09
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There have been some studies at home and abroad, but there have been no reports of high-density, high-activity, and large-area microecological preparations

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Follow these steps:

[0021] 1) Activate each slant strain stored at 4°C, and culture them in shake flasks respectively. Both Bacillus megaterium and Streptomyces luteus were cultured with soluble starch medium, and the specific formula was as follows: soluble starch 20g, KNO 3 1g, NaCl 0.5g, K 2 HPO 4 0.5g, MgSO 4 .7H 2 O 0.5g, FeSO 4 0.01g, distilled water 1000mL, pH7.2, autoclave at 121℃ for 20 minutes. For Bacillus megaterium, culture at 37°C for 24 hours; for Streptomyces luteus, culture at 30°C for 48 hours.

[0022] 2) Level-1 liquid expansion culture (Bacillus megaterium and Streptomyces flavinus were separately expanded culture, the culture medium was cornstarch, and the culture conditions were the same as above); the inoculum size was 5%.

[0023] 3) The secondary liquids are respectively expanded and cultured, and the culture medium and culture conditions are the same as above; the inoculum size is 5%.

[0024] 4) Using wheat bran as the carrier to...

Embodiment 2

[0027] Follow these steps:

[0028] 1) Activate each slant strain stored at 4°C, and culture them in shake flasks respectively. Both Bacillus megaterium and Streptomyces luteus were cultured with soluble starch medium, and the specific formula was as follows: soluble starch 20g, KNO 3 1g, NaCl 0.5g, K 2 HPO 4 0.5g, MgSO 4 .7H 2 O 0.5g, FeSO 4 0.01g, 1000ml distilled water, pH7.2, autoclave at 121℃ for 20 minutes. For Bacillus megaterium, culture at 37°C for 24 hours; for Streptomyces luteus, culture at 30°C for 48 hours.

[0029] 2) First-level liquid expansion culture (Bacillus megaterium and Streptomyces flavinus were respectively expanded separately, the culture medium was cornstarch, and the culture conditions were the same as above); the inoculum size was 10%.

[0030] 3) The secondary liquids are respectively expanded and cultivated, and the culture medium and culture conditions are the same as above; the inoculum size is 10%.

[0031] 4) Using wheat bran as th...

Embodiment 3

[0034] Follow these steps:

[0035] 1) Activate each slant strain stored at 4°C, and culture them in shake flasks respectively. Both Bacillus megaterium and Streptomyces luteus were cultured with soluble starch medium, and the specific formula was as follows: soluble starch 20g, KNO 3 1g, NaCl 0.5g, K 2 HPO 4 0.5g, MgSO 4 .7H 2 O 0.5g, FeSO 4 0.01g, 1000ml distilled water, pH7.2, autoclave at 121℃ for 20 minutes. For Bacillus megaterium, culture at 37°C for 24 hours; for Streptomyces luteus, culture at 30°C for 48 hours.

[0036] 2) First-level liquid expansion culture (Bacillus megaterium and Streptomyces flavinus were respectively expanded separately, the culture medium was cornstarch, and the culture conditions were the same as above); the inoculum size was 10%.

[0037] 3) The secondary liquids are respectively expanded and cultured, and the culture medium and culture conditions are the same as above; the inoculum size is 5%.

[0038] 4) Using wheat bran as the ...

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PUM

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Abstract

The present invention relates to a microecological germicide preparation for killing soil eelworm and its preparation method. Said microecological germicide preparation for killing soil eelworm is made up by using (by wt%) 60%-80%, of bacillus megatherium and 20%-40% of streptolin through a certain preparation process. The soil eelworm-killing rate can be up to 100% and the soil grub-killing rate can be up to above 95%.

Description

technical field [0001] In the field of biological technology, the present invention relates to a microbial ecological bacteria preparation for killing soil nematodes and a preparation method thereof. Background technique [0002] At present, the soil ecosystem in our country has been or is being severely damaged, soil nematodes have increased significantly, and the types and scope of plant diseases have continued to increase. These are all evidences of the destruction of the soil ecosystem. Among them, soil nematodes are soil pests that directly affect the yield of crops, especially in the soil where peanuts and sweet potatoes are planted. Nematodes can eat up or destroy peanuts and sweet potatoes in a whole piece of land, and the harvest is almost impossible. In the past, only chemical pesticides were sought, but they were effective in the first year and almost ineffective in the second year, and the soil pollution it caused could not be eliminated for decades. Therefore, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P5/00
Inventor 张清敏崔合香崔小会刘曼吉雪莹
Owner NANKAI UNIV
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