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Maize flour low-temperature synchronous saccharification technique by double enzymatical process and its application

A technology of simultaneous saccharification and corn flour, applied in the fields of biofuel and fermentation, can solve the problems of high energy consumption and long reaction time, and achieve the effects of low energy consumption, low production cost and high glucose conversion rate.

Inactive Publication Date: 2008-01-02
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the reaction time of the two-step process is longer and the energy consumption is larger

Method used

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  • Maize flour low-temperature synchronous saccharification technique by double enzymatical process and its application
  • Maize flour low-temperature synchronous saccharification technique by double enzymatical process and its application
  • Maize flour low-temperature synchronous saccharification technique by double enzymatical process and its application

Examples

Experimental program
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Embodiment Construction

[0041] Embodiments 1-6 use a planetary ball mill (XQM-4L, Nanjing Institute of Experimental Instruments) to wet-grind peeled and degerminated corn flour for 3 hours to prepare a fine corn flour slurry. The mass ratio of corn flour to water is 1: 3; use a particle size distribution analyzer (2000, MALVERN, UK) to measure the particle size distribution of corn flour, and the median particle size is 14.6 μm: take the above-mentioned corn flour slurry equivalent to 60 g of dry matter in a container, and adjust the concentration 10% corn flour feed liquid; adjust the pH value of feed liquid to 4.5, 5.0, 5.5, 6.0, 6.5, 7.0 with acid solution and alkali solution; add α-amylase 10u / g to feed liquid, saccharification Enzyme 200u / g (based on dry matter of corn flour); put the container in a water bath at 60°C, and use an electric mixer to stir the feed liquid evenly, and the saccharification time is controlled at 24 hours; sample 3ml at intervals of 1 hour, and use a biosensing analyzer ...

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Abstract

The invention discloses a low-temperature synchronous saccharifying technique and application of maize flour, which comprises the following steps: blending the maize flour and water; grinding through wet method; making slurry of fine maize flour; modulating the slurry density; adjusting pH value; adding alpha-amylase and saccharifying enzyme into the slurry simultaneously; controlling the saccharifying temperature at 30 deg. c and time; making the glucose liquid synchronously.

Description

technical field [0001] The invention relates to a corn flour saccharification process, in particular to a corn flour low-temperature synchronous saccharification process and its application. Background technique [0002] Corn flour contains 70% to 75% of starch, about 12% of protein, 3% to 6% of fat, about 2% of vitamins and other nutrients. Corn starch is mainly amylopectin and exists in the form of granules. The diameter of cornstarch granules is about 0.02mm, and they are various in shape, tightly packed, and surrounded by network structures such as protein, fat, vitamins, and inorganic salts. The nitrogen content of corn is almost all true protein, and it is mainly gliadin, and there is no water-soluble protein. Therefore, corn flour is more difficult to saccharify than starch alone. [0003] Double enzymatic saccharification of corn flour mainly includes two stages of liquefaction and saccharification [Li Dapeng, Luo Wenbin. Research on the process conditions of liqu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/02C12P19/14C12P7/06
CPCY02E50/17Y02E50/10
Inventor 缪冶炼吴琴燕
Owner NANJING UNIV OF TECH
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