Kit for testing tiny gene loss of male Y chromosome, and testing method

Abstract

This invention relates to test kit and method for detecting male Y chromosome microdeletion genes. The test kit contains 30 primers for amplifying AZF of Y chromosome, and 2 primers for amplifying male-specific Sry gene. The test kit can be used for detecting male Y chromosome microdeletion genes, and has such advantages as high efficiency, low cost and stable result.

Description

technical field [0001] The invention relates to the field of gene deletion detection, in particular to a male Y chromosome microdeletion gene detection kit and detection method. Background technique [0002] About 15% of couples in the world are plagued by infertility, and male factors account for about half of them. After excluding vas deferens obstruction, Klinefelter's disease, and other genitourinary causes, the most likely cause of severe oligospermia and azoospermia is abnormal spermatogenesis genes. With regard to spermatogenesis genes, particular attention was paid to the 11 interval on the long arm of the Y chromosome. The Y chromosome constitutes 2% of the human genome and consists of a short arm (Yp) and a long arm (Yq). Cytogenetic and molecular biological studies of azoospermia and severe oligospermia have also confirmed the existence of azoospermia factor (AZF) on the Y chromosome, which has four clear, non-overlapping subregions, namely AZFa, AZFb, AZFc and...

AI Technical Summary

Problems solved by technology

[0004] 1. Multiplex PCR electrophoresis: Multiplex PCR is currently the most widely used method for the detection of Y chromosome microdeletions. It is simple, fast, sensitive, specific, economical and high-throughput. It is an ideal method for detecting AZF. Compared with higher technical requirements and lack of standard screening protocol, the results of each laboratory are not the same

[0005] 2. Probe technology: Southern blot or Northern blot with specific probes is an effective method to study the relationship between AZF microdeletion and male infertility, but the method is too cumbersome and difficult to promote, and it is mostly used in laboratory research

(2) Capillary electrophoresis technology: it has the advantages of high throughput, high sensitivity, fast speed and less sample volume, but requires a special capillary electrophoresis instrument

[0007] Chinese patent 200410043918.0 discloses two pairs of primer sequences for specifically amplifying sY87 and sY143 in the microdeletion STS on the Y chromosome, but as a method for detecting the microdeletion of the Y chromosome, it does not meet the requirements of the European Reproductive Society to detect at least 6 STS (sY254, sY255, sY84, sY86, sY127, sY134) minimum requirements

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