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Preparation method for producing microbe polysaccharides formulation using lactobacillus and microzyme

A microbial polysaccharide and lactobacillus technology, which is applied in biochemical equipment and methods, microbial determination/inspection, bacteria, etc., can solve the problems of serious breeding biological diseases, increasing pathogenic microorganism species, and breeding ecological environment damage, etc. Non-specific immunity, improving immunity, the effect of increasing immunity

Inactive Publication Date: 2007-12-12
SHANGHAI CHUANGBO ECOLOGICAL ENG
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  • Summary
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] With the vigorous development of aquaculture intensification in our country, the pollution of aquaculture water environment is becoming more and more serious. The variety of microorganisms increases and the speed of transmission accelerates, and the occurrence of biological diseases in aquaculture is becoming more and more serious, causing serious losses to the aquaculture industry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037]Step 1: Breeding of Fermentation Strains

[0038] a. Screening of strains: Extract secretions from healthy crucian carp intestinal tract as bacterial source, use MRS lactic acid bacteria medium to isolate lactic acid bacteria, screen out Lactobacillus plantarum, and use yeast selection medium to isolate Saccharomyces cerevisiae in beer fermentation broth; isolate The Lactobacillus plantarum and Saccharomyces cerevisiae were respectively stored in the yeast extract wort agar medium at 4°C; the present invention uses the following strains screened:

[0039] Lactobacillus plantarum (ATCC8014) Lactobacillus plantarum, Saccharomycscerevisiac (IFO0203) Saccharomyces cerevisiae,

[0040] b. Inoculate the isolated Lactobacillus plantarum and Saccharomyces cerevisiae into the broth medium respectively, and after culturing at 37°C for 22 hours, inoculate Saccharomyces cerevisiae on the yeast extract malt juice agar medium, and inoculate the Lactobacillus plantarum In the lactic a...

Embodiment 2

[0064] The first step: breeding of fermented strains: same as Example 1;

[0065] Step Two: Liquid Culture of Beneficial Bacteria

[0066] a. Weigh each strain according to the following weight ratio: Lactobacillus plantarum 65%, Saccharomyces cerevisiae 35%

[0067] b. Mix the weighed Lactobacillus plantarum and Saccharomyces cerevisiae together for compound culture: the mixed lawn weighed in step a is inoculated in a medium sterilized at 121° C. for 45 minutes at a ratio of 1:30. Stir in the fermenter for 30min at a speed of 200r / min. After stirring evenly, put it into a sterilized plastic bucket and let it stand for 7 days. During the standstill, measure the Ph value every day and release the produced gas;

[0068] c. Sampling 3 times during the fermentation process, detecting the growth of the bacteria and measuring the Ph value under a microscope, and stopping the fermentation when the total number of viable bacteria reaches 2 billion / ml concentration and the Ph value re...

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PUM

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Abstract

The invention relates to a method for preparing microbiological polysaccharide product by using lactic acid bacillus and yeast. It is characterized in that it employs liquid culture of benefitial bacteria, collects bacteria cell wall through centrifugation, solid enlargement culturing bacteria group, adding polysaccharide- rich cell wall into culture medium. The high- concentration lactic acid bacteria in product can distinctively improve digestion and absorption for aquatic animal, generated peptidoglycan increases immunity of aquatic animal to pathogenesis; high- concentration yeast in the product can increase single-cell protein amount needed for fish growth, the yeast cell is broke, and releases a large amount of beta- 1, 3- dextran, which can apparently increase immunocompetence for Gram-positive bacteria and Gram-negative bacteria. The invention employs grain crop and commonly used feedstuff carrier as main raw material for culture medium, which reduces production cost, and fermentation device and production equipment are commonly used, which is easy for spread in aquaculture.

Description

technical field [0001] The invention relates to a preparation method for producing microbial polysaccharide preparations by using lactobacillus and yeast, which can be used to improve the immunity of farmed animals such as livestock, poultry and aquatic products, and belongs to the technical field of agricultural biological products. Background technique [0002] With the vigorous development of aquaculture intensification in our country, the pollution of aquaculture water environment is becoming more and more serious. The variety of microorganisms has increased and the speed of transmission has accelerated, and the occurrence of biological diseases in aquaculture has become more and more serious, causing serious losses to the aquaculture industry. According to incomplete statistics, the area of ​​aquaculture diseases above the middle level in the country accounts for 15% to 20% of the total aquaculture area every year, and the output loss exceeds 1 million tons. Aquatic an...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12P19/04C12P1/04C12P1/02C12Q1/25
Inventor 江瀚
Owner SHANGHAI CHUANGBO ECOLOGICAL ENG
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