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Biological artificial blood vessel capable of in vivo capturing endothelial ancestral cell

An endothelial progenitor cell and bioartificial technology, applied in the direction of microorganisms, animal cells, vertebrate cells, etc., can solve unseen problems, achieve good biocompatibility, resist intravascular thrombosis, and inhibit pathological proliferation

Active Publication Date: 2007-11-07
广州宏畅生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no research report on bioartificial blood vessels captured in vivo by epithelial progenitor cells at home and abroad.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1. Preparation of a bioartificial blood vessel capable of capturing endothelial progenitor cells in vivo. The vascular matrix material was prepared according to the above-mentioned step 1, and after surface modification was carried out according to step 3, animal experiments were carried out according to step 4.

[0045] The obtained bioartificial blood vessel was transplanted into the internal iliac artery of minipigs. After 7 days, fluorescent labeling laser confocal detection of endothelial progenitor cells was performed. It can be seen that the vascular cavity is densely covered with vascular endothelial progenitor cells. The normal morphology, dense arrangement, and distribution along the long axis of the blood vessel indicated that under the above conditions, the endothelial phase cells were successfully planted in the blood vessel, and silver staining also showed that the endothelial progenitor cells were successfully planted on the vascular matrix. Fluore...

Embodiment 2

[0048] Example 2. Preparation of a bioartificial blood vessel capable of capturing endothelial progenitor cells in vivo. The vascular matrix material was prepared according to the above-mentioned step 1, and after the surface modification was carried out according to the step 3, the animal experiment was carried out according to the step 4.

[0049] The obtained small-diameter bioartificial blood vessels were transplanted into the canine common carotid artery, and tested 6 months later. It can be seen that the distribution of vascular endothelial cells is intact and the shape is normal. There are a large number of smooth muscle cells in the media along the long axis of the blood vessels.

[0050]Compared with small-diameter expanded polytetrafluoroethylene artificial vascular grafts. It can be seen that part of the expanded polytetrafluoroethylene artificial blood vessel lumen is completely occluded, there is thrombus formation in the blood vessel, no hyperplasia is seen, and ...

Embodiment 3

[0052] Example 3. Preparation of a bioartificial blood vessel capable of capturing endothelial progenitor cells in vivo. The vascular matrix material was prepared according to the above-mentioned step 2. After surface modification was carried out according to step 3, animal experiments were carried out according to step 4.

[0053] The obtained decellularized bioartificial blood vessel was transplanted into the canine external iliac artery after surface modification. After 7 days, the fluorescence-labeled laser confocal detection of endothelial progenitor cells was performed. It can be seen that the vascular lumen is densely covered with vascular endothelial progenitor cells. The luminal endothelial progenitor cells were normal in shape, densely arranged, and distributed along the long axis of the blood vessel, indicating that under the above conditions, the blood vessels were successfully implanted with endothelial progenitor cells. Fluorescence microscopy showed that the endot...

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Abstract

The present invention relates to preparation of biological artificial blood vessel capable of in vivo capturing endothelial ancestral cell, and features that the biological artificial blood vessel may be prepared with composite blood vessel material or homogeneous or heterogeneous blood vessel with the antigen eliminated and through surface modification. The biological artificial blood vessel may be used as tissue engineering blood vessel rack, on which smooth muscle cell and / or endothelial cell may be planted. The biological artificial blood vessel of the present invention has excellent mechanical performance and anticoagulant property, and may be in different calibers. It may be in vivo reshaped and in vivo induced to plant endothelial ancestral cell. It can antagonize intravascular thrombogenesis and inhibit the pathological proliferation of smooth muscle cell to avoid angiostenosis and blood vessel blocking.

Description

technical field [0001] The invention relates to bioengineering technology, in particular to a bioartificial blood vessel capable of capturing autologous circulating blood endothelial progenitor cells in vivo. technical background [0002] In the past few decades, the development of vascular graft materials has gone through several stages such as calf blood vessels, polyester products, human umbilical veins, expanded polytetrafluoroethylene, and autologous great saphenous veins. For large blood vessels, although PTFE (polyester Tetrafluoroethylene) and other artificial blood vessels can meet clinical needs to a certain extent, but they cannot be degraded, and exist in the human body as foreign bodies all the time, and inhibit the regeneration function of vascular cells. The anticoagulant mechanism of artificial blood vessels such as PTFE is to allow blood to form a layer of thrombus membrane on the inner wall of the artificial blood vessel. Therefore, the inner diameter of th...

Claims

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Application Information

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IPC IPC(8): A61L29/12A61L29/16A61L33/06C12N5/08C12N11/00C12N5/071
Inventor 朱楚洪应大君糜建红李立侯春丽
Owner 广州宏畅生物科技有限公司
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