Livestock embryo vitrifying freeze process on metal surface

Abstract

The present invention is livestock embryo vitrifying freeze process on metal surface, and belongs to the field of biotechnology. The livestock embryo vitrifying freeze process includes replacing freeze protectant for liquid inside and outside the embryo cell and fast freezing of the embryo liquid drop. During the fast freezing, the embryo liquid drop is dropped onto the exposed upper surface of one metal block with lower part soaked inside liquid nitrogen. The present invention can cool embryo in high speed while avoiding contamination of impurity in liquid nitrogen to the embryo. Besides, adding cytochalasin B as cytoskeleton protectant into the frozen liquid can raise the freezeproof capacity of the embryo and raise vitrification freezing effect. The present invention is used for freezing livestock embryo.

Description

technical field [0001] The invention relates to the technical field of embryo biology, in particular to a vitrification freezing method of livestock embryos. Background technique [0002] With the development of superovulation technology, in vitro fertilization, embryo transfer technology and animal cloning technology, livestock embryos have become commercial products. Embryo cryopreservation technology is extremely important for the commercial application of embryos and the promotion of cloning technology. Livestock embryo freezing technology has become a research hotspot in embryo engineering technology. In recent years, embryo cryopreservation technology, especially vitrification, has developed rapidly. Vitrification technology is the result of the combination of rapid freezing and high-concentration cryoprotectant. The high-concentration cryoprotectant forms a transparent glass-like solid during rapid cooling. During the cooling process, the viscosity is very high, but ...

AI Technical Summary

Problems solved by technology

Due to the existence of microtubules in the microtube method, the freezing speed of embryos is reduced, which affects the efficiency of cryopreservation

The conventional operation of the micro-drop method is to immerse the embryo droplets directly into the liquid nitrogen. Since the liquid nitrogen may contain some pathogenic microorganisms, the direct contact of the embryo droplets with the liquid nitrogen will cause contamination of the embryos. In liquid nitrogen, due to the temperature difference, the liquid nitrogen around the embryo droplet is easy to vaporize and form an air mass, which affects the cooling rate

In the process of embryo freezing, the low temperature will cause certain damage to the cytoskeleton of the embryo, which weakens the antifreeze ability of the embryo to a certain extent.

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